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Journals / Türk Biyokimya Dergisi / 2017 / Cilt: 42 - Sayı: 5

Mide Kanserli Hastalarda CTLA-4 ve CD28 Gen Varyantlarının ve Dolaşımdaki Düzeylerinin Değerlendirilmesi

Department of Gastroenterology, Istanbul, Turkey Ammad Ahmad Farooqi: Rashid Latif Medical College, Department of Gastroenterology, Istanbul Medical Faculty, Lahore, Pakistan Bayram Kıran: Medical Faculty of Kastamonu University, Department of Biology, Kastamonu, Turkey

İlhan YAYLIM
Özlem KÜÇÜKHÜSEYİN
Soykan ARİKAN
Bayram KIRAN
Saime TURAN
Cihan COŞKUN
Canan CACİNA
Ahmad FAROOQİ
Alper GÜMÜŞ
Pages
551–558
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We examined the influence of critical polymorphisms of CTLA-4 and CD28 genes and circulating protein levels on the etiology of gastric cancer.Methods: Genotyping of SNPs was performed in 55 gastric cancer patients and 105 healthy individuals using the PCR-RFLP method, and circulating levels of sCTLA-4 and sCD28 were measured

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Gastric cancer is one of the most common malignancies worldwide.
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Key Findings & Data:The risk factors for gastric cancer include environmental and genetic factors.
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Conclusion & Impact:We examined the influence of critical polymorphisms of CTLA-4 and CD28 genes and circulating protein levels on the etiology of gastric cancer.Methods: Genotyping of SNPs was performed in 55 gastric cancer patients and 105 healthy individuals using the PCR-RFLP method, and circulating levels of sCTLA-4 and sCD28 were measured
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Gastric cancer is one of the most common malignancies worldwide. The risk factors for gastric cancer include environmental and genetic factors. Inflammation and the immune system are known to contribute to the development of the gastric cancer. We examined the influence of critical polymorphisms of CTLA-4 and CD28 genes and circulating protein levels on the etiology of gastric cancer.Methods: Genotyping of SNPs was performed in 55 gastric cancer patients and 105 healthy individuals using the PCR-RFLP method, and circulating levels of sCTLA-4 and sCD28 were measured

Özet

Amaç: Mide kanseri dünyadaki en yaygın kötü huylu tümörlerden biridir. Genetik ve çevresel etkenlere bağlı olarak hastalığın görülme riski artabilir. İnflamasyon ve bağışıklık sisteminin mide kanseri gelişimine katkıda bulunduğu bilinmektedir. Biz bu çalışmada CTLA-4 ve CD28 genlerindeki önemli polimorfizmleri ve bu genlerin ürünlerinin dolaşımdaki düzeylerinin mide kanseri etiyolojisine etkisini araştırdık.Yöntemler: 55 mide kanserli hasta ve 105 sağlıklı bireyin tek nükleotid polimorfizmlerinin (SNP) genotiplemesi PCR-RFLP yöntemi kullanılarak gerçekleştirildi. Dolaşımdaki sCTLA-4 ve sCD28 düzeyleri ölçüldü.Bulgular: Değerlendirilen SNP'lerin [CTLA-4 +49 A > G (Rs231775), CTLA-4 -318 C > T (Rs5742909), CD28 C > T (Rs3116496)] genotip ve alel dağılımlarında hasta ve sağlıklı bireyler arasında anlamlı bir fark yoktu (p = 0.36, p = 0.78, p = 0.80). Ancak sCTLA-4 ve sCD28 düzeylerinin mide kanserli hastalarda anlamlı yüksek olduğu gözlendi (p < 0.001, p < 0.001).Sonuç: Çalışmamızda değerlendirilen polimorfizmlerin mide kanserine yatkınlık oluşturmadıkları sonucuna ulaştık. Öte yandan sCTLA-4 ve sCD28'in mide kanserli hastaların tanısında ve izlenmesinde yardımcı parametreler olarak kullanılabileceğini düşünüyoruz

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Cite This Article

YAYLIM, İ., KÜÇÜKHÜSEYİN, Ö., ARİKAN, S., KIRAN, B., TURAN, S., COŞKUN, C., CACİNA, C., FAROOQİ, A., GÜMÜŞ, A. (2022). Mide Kanserli Hastalarda CTLA-4 ve CD28 Gen Varyantlarının ve Dolaşımdaki Düzeylerinin Değerlendirilmesi. Türk Biyokimya Dergisi, Cilt: 42 - Sayı: 5, 551–558. https://ajindex.com/articles/620797

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