SavedAdd your journal

Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 2

Genome-wide identification, phylogeny and expression analysis of G6PC gene family in common carp, Cyprinus carpio

G6PC gen ailesinin sazan, Cyprinus carpio,balığındaki filogenisin ve ekspresyonunun analizi

Sijia LIU
Kai ZHAO
Zhigang QIAO
Cunfang ZHANG
Fei TIAN
Pages
91–98
Publication Date
2022-11-06
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The expression patterns revealed that only cg6pca.2a elevated significantly after 12 and 24 h of both starvation and cold treatment (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The Glucose 6-phosphatase (G6Pase) catalytic subunit (G6PC) catalyzes glucose 6-phosphate (G6P) to inorganic phosphate and glucose, playing a critical role in endogenous energy supply.
📊
Key Findings & Data:Here, the G6PC gene family was investigated and characterized in common carp (Cyprinus carpio).
💡
Conclusion & Impact:The expression patterns revealed that only cg6pca.2a elevated significantly after 12 and 24 h of both starvation and cold treatment (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The Glucose 6-phosphatase (G6Pase) catalytic subunit (G6PC) catalyzes glucose 6-phosphate (G6P) to inorganic phosphate and glucose, playing a critical role in endogenous energy supply. Here, the G6PC gene family was investigated and characterized in common carp (Cyprinus carpio). Methods: Sequence alignment and phylogenetic analysis were performed using MEGA5. The HMM profiles, motif structure were analyzed using Pfam and MEME, respectively. Quantitative real-time PCR was used to test the expression profiles. Results: Four assumptive members of G6PC family in common carp whole-genome sequence were identified as cg6pca.1, cg6pca.2a, cg6pca.2b and cg6pcb which were classified into g6pca and g6pcb subtypes, respectively. Evolutionary analysis revealed that cg6pca.2a and cg6pca.2b have a closer evolutionary relationship, and the same subtype members have higher homology among different species. A classical PAP2-glucose phosphates domain is found in four genes and were highly conserved. The expression patterns revealed that only cg6pca.2a elevated significantly after 12 and 24 h of both starvation and cold treatment (p

Özet

Amaç: Glikoz 6-fosfatazın (G6Pase) katalitik alt birimi (G6PC), glikoz 6-fosfat (G6P)’ı inorganik fosfat ve glukoza katalize ederek endojen enerji kaynağında kritik bir rol oynar. Buradaki G6PC gen ailesi, sazanlarda (Cyprinus carpio) araştırılmış ve karakterize edilmiştir. Yöntemler: Dizileme ve filogenetik analiz MEGA5 kullanılarak yapılmıştır. HMM profilleri, motif yapısı sırasıyla Pfam ve MEME kullanılarak analiz edilmiştir. Ekspresyon profillerini test etmek için kantitatif gerçek zamanlı PCR kullanılmıştır. Bulgular: Sazanların tüm genom dizisinde G6PC ailesinin dört varsayım üyesi, sırasıyla g6pca ve g6pcb alt tipleri olarak sınıflandırılan cg6pca.1, cg6pca.2a, cg6pca.2b ve cg6pcb olarak tanımlanmaktadır. Evrimsel analizler, cg6pca.2a ve cg6pca.2b’nin daha evrimsel bir ilişkiye sahip olduğunu ve aynı alt tipteki üyelerin farklı türler arasında daha yüksek homolojiye sahip olduğunu ortaya çıkarmıştır. Dört gende klasik bir PAP2-glukoz fosfat bölgesi bulunur ve bu yüksek oranda korunmuştur. Ekspresyon paternleri, hem cg6pca.2a’nın hem açlık hem de soğuk tedavisinden 12 ve 24 saat sonra anlamlı derecede yükseldiğini ortaya koymuştur (p

🔖

Cite This Article

LIU, S., ZHAO, K., QIAO, Z., ZHANG, C., TIAN, F. (2022). Genome-wide identification, phylogeny and expression analysis of G6PC gene family in common carp, Cyprinus carpio. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 2, 91–98. https://ajindex.com/articles/620789

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Ghosh A, Shieh JJ, Pan CJ, Sun MS, Chou JY. The catalytic center of glucose-6-phosphatase. HIS176 is the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis. J Biol Chem 2002;277:32837–42
  2. Enes P, Panserat S, Kaushik S, Oliva-Teles A. Nutritional regulation of hepatic glucose metabolism in fish. Fish Physiol Biochem 2009;35:519–39
  3. Moon TW, Foster GD. Chapter 4 tissue carbohydrate metabolism, gluconeogenesis and hormonal and environmental influences. Biochem Mol Bio Fish 1995;4:65–100
  4. Hutton JC, O’Brien RM. Glucose-6-phosphatase catalytic subunit gene family. J Biol Chem 2009;284:29241–5
  5. Wang Y, Wang H, Li M, Gao Z, Zhang S. Identification, expression and regulation of amphioxus G6Pase gene with an emphasis on origin of liver. Gen Comp Endocrinol 2015;214:9–16
  6. Panserat S, Médale F, Blin C, Brèque J, Vachot C, Plagnes-Juan E, et al. Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout (Oncorhyncus mykiss), gilthead seabream (Sparus aurata) and common carp (Cyprinus carpio). Am J Physiol Regul Integr Comp Physiol 2000;278:R1164–70
  7. Marandel L, Seiliez I, Véron V, Skibacassy S, Panserat S. New insights into the nutritional regulation of gluconeogenesis in carnivorous rainbow trout (Oncorhynchus mykiss): a gene duplication trail. Physiol Genomics 2015;47:253–63
  8. Kolder IC, van der Plas-Duivesteijn SJ, Tan G, Wiegertjes GF, Forlenza M, Guler AT, et al. A full-body transcriptome and proteome resource for the European common carp. BMC Genomics 2016;17:701
  9. Xu P, Zhang X, Wang X, Li J, Liu G, Kuang Y, et al. Genome sequence and genetic diversity of the common carp, Cyprinus carpio. Nat Genetics 2014;46:1212–9
  10. Magnoni LJ, Scarlato NA, Ojeda FP, Wöhler OC. Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish. Polar Biol 2013;36:661–71
  11. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA, Mcwilliam H, et al. Clustal w and clustal x version 2.0. Bioinformatics 2007;23:2947–8
  12. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. Mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2014;28:2731–9
  13. Peri S, Steen H, Pandey A. Gpmaw – a software tool for analyzing proteins and peptides. Trends Biochem Sci 2001;26:687–9
  14. Bailey TL, Boden M, Buske FA, Frith M, Grant CE, Clementi L, et al. Meme suite: tools for motif disc