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Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 6

Effect of static magnetic field with quercetin and hesperetin on MCF-7 and MDA MB-231 breast cancer cells

[Kuersetin ve Hesperetin ile Statik Manyetik Alanın MCF-7 ve MDA MB-231 Meme Kanseri Hücrelerine Etkisi]

Bahadır ÖZTÜRK
Gülsüm ABUSOĞLU
Pages
833–842
Publication Date
2022-11-06
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Apoptosis rates andBax, Bcl-2 protein levels were detected by flow cytometerand Western Blot, respectively.Results: Cell lines were treated with quercetin and quercetin+ SMF, substantial amount of cells [3.96, 4.86, 11.40%for MCF-7 and MDA MB-231 cell lines, respectively(p

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Static magnetic field (SMF) was previously inpractice for the therapy of some diseases and it has beenthought that it may be a reliable supportive technique.
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Key Findings & Data:Theaim of this study was to find out the synergistic effect ofSMF administration with flavonoids in terms of apoptosison breast cancer cell lines.Material and methods: The effects of flavonoids on theproliferation of breast cancer cell lines were observed byMTT cell viability test.
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Conclusion & Impact:Apoptosis rates andBax, Bcl-2 protein levels were detected by flow cytometerand Western Blot, respectively.Results: Cell lines were treated with quercetin and quercetin+ SMF, substantial amount of cells [3.96, 4.86, 11.40%for MCF-7 and MDA MB-231 cell lines, respectively(p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Static magnetic field (SMF) was previously inpractice for the therapy of some diseases and it has beenthought that it may be a reliable supportive technique. Theaim of this study was to find out the synergistic effect ofSMF administration with flavonoids in terms of apoptosison breast cancer cell lines.Material and methods: The effects of flavonoids on theproliferation of breast cancer cell lines were observed byMTT cell viability test. The cells were treated withSMF + hesperetin and SMF + quercetin. Apoptosis rates andBax, Bcl-2 protein levels were detected by flow cytometerand Western Blot, respectively.Results: Cell lines were treated with quercetin and quercetin+ SMF, substantial amount of cells [3.96, 4.86, 11.40%for MCF-7 and MDA MB-231 cell lines, respectively(p

Özet

Amaç: Statik manyetik alan (SMA) daha önce bazı hastalıkların tedavisi için bir uygulama alanıydı ve güvenilir destekleyici tedavi tekniği olabileceği düşünülmekteydi. Bu çalışmanın amacı, flavonoidlerle beraber SMA uygulamasının meme kanseri hücre serilerinin apopitoz üzerindeki sinerjistik etkisini bulmaktır. Gereç ve Yöntemler: Flavonoidlerin meme kanseri hücre serilerinin proliferasyonu üzerindeki etkileri MTT hücre canlılık testi ile gözlenmiştir. Hücreler SMA + hesperetin ve SMA + kuersetin ile muamele edilmiştir. Apopitoz oranı ve Bax, Bcl-2 protein seviyeleri sırasıyla akış sitometresi ve Western Blot ile tespit edilmiştir. Bulgular: Hücre hatları quercetin ve quercetin + SMF ile tedavi edildi, esasen önemli miktarda hücre [sırasıyla MCF- 7 ve MDA MB-231 hücre hatları için % 3.96, % 4.86 ve % 9.70, % 11.40 (p

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Cite This Article

ÖZTÜRK, B., ABUSOĞLU, G. (2022). Effect of static magnetic field with quercetin and hesperetin on MCF-7 and MDA MB-231 breast cancer cells. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 6, 833–842. https://ajindex.com/articles/620780

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References (14)

  1. Chien SY, Wu YC, Chung JG, Yang JS, Lu HF, Tsou MF, et al. Quercetin-induced apoptosis acts through mitochondrial- and caspase-3-dependent pathways in human breast cancer MDA-MB-231 cells. Hum Exp Toxicol 2009;28:493–503
  2. Choi EJ. Hesperetin induced G1-phase cell cycle arrest in human breast cancer MCF-7 cells: involvement of CDK4 and p21. Nutr Canc 2007;59:115–9
  3. Choi EJ, Bae SM, Ahn WS. Antiproliferative effects of quercetin through cell cycle arrest and apoptosis in human breast cancer MDA-MB-453 cells. Arch Pharm Res 2008;31:1281–5
  4. Ye L, Chan FL, Chen S, Leung LK. The citrus flavonone hesperetin inhibits growth of aromatase-expressing MCF-7 tumor in ovariectomized athymic mice. J Nutr Biochem 2012;23:1230–7
  5. Shirzad M, Heidarian E, Beshkar P, Gholami-Arjenaki M. Biological effects of hesperetin on interleukin-6/phosphorylated signal transducer and activator of transcription 3 pathway signaling in prostate cancer PC3 cells. Pharmacogn Res 2017;9: 188–94
  6. Turati F, Carioli G, Bravi F, Ferraroni M, Serraino D, Montella M, et al. Mediterranean diet and breast cancer risk. Nutrients 2018; 10:326
  7. Parhiz H, Roohbakhsh A, Soltani F, Rezaee R, Iranshahi M. Antioxidant and anti-inflammatory properties of the citrus flavonoids hesperidin and hesperetin: an updated review of their molecular mechanisms and experimental models. Phytother Res 2015;29:323–31
  8. Roohbakhsh A, Parhiz H, Soltani F, Rezaee R, Iranshahi M. Molecular mechanisms behind the biological effects of hesperidin and hesperetin for the prevention of cancer and cardiovascular diseases. Life Sci 2015;1:64–74
  9. Gibellini L, Pinti M, Nasi M. Quercetin and cancer chemoprevention. Evidence-based complementary and alternative medicine. eCAM 2011;2011:591356
  10. Zhang M, Swarts SG, Yin L, Liu C, TianY, Cao Y, et al. Antioxidant properties of quercetin. Oxyg Trans Tissue 2011;701:283–9
  11. Ozturk B, Durak ZE, Buber S, Kocaoglu EH. Effect of static magnetic field on oxidant/antioxidant parameters in cancerous and noncancerous human gastric tissues. Scientifica 2016;2016: 8608462
  12. Ghodbane S, Lahbib A, Sakly M, Abdelmelek H. Bioeffects of static magnetic fields: oxidative stress, genotoxic effects, and cancer studies. BioMed Res Int 2013;2013:602987
  13. Sun RG, Chen WF, Qi H, Zhang K, Bu T, Liu Y, et al. Biologic effects of SMF and paclitaxel on K562 human leukemia cells. Gen Physiol Biophys 2012;31:1–10
  14. Aljarrah K, Mhaidat NM, Al-Akhras MA, Aldaher AN, Albiss B, Aledealat