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Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 6

The effects of silibinin on oxidative stress and microRNA-10b expression in animal models of breast cancer

[Meme Kanseri Hayvan Modellerinde Silibininin Oksidatif Stres ve MicroRNA-10b Ekspresyonu Üzerine Etkileri]

Farhad SOLEİMANİ
Rasoul SHARİFİ
Minoo MAHMOODİ
Seyed Mehrdad KASSAEE
Pages
707–714
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Data were analyzed using one-wayANOVA, Kruskal–Wallis, Mann–Whitney and t-test (p

📌 3 Key Takeaways

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Objective & Hypothesis:Background and aims: Among the factors associated withcancer are the oxidative stress and increased expression ofsome microRNA (miRs).
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Key Findings & Data:Silibinin has an anti-tumor effect.Therefore, this study evaluates the effects of silibinin onoxidative stress indices and miR-10b expression in theanimal models of breast cancer.Material and methods: In this study, 48 Balb/c mice weredivided into six groups (each group contains eight mice): thehealthy control, the cancer control, the healthy groupreceiving 20mg of silibinin, the cancer group receiving 20 mgof silibinin, the cancer group receiving 40mg of silibinin andthe cancer group receiving 80mg of silibinin for three weeks.In order to induce cancer, 4T1 cell line was used.
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Conclusion & Impact:Data were analyzed using one-wayANOVA, Kruskal–Wallis, Mann–Whitney and t-test (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background and aims: Among the factors associated withcancer are the oxidative stress and increased expression ofsome microRNA (miRs). Silibinin has an anti-tumor effect.Therefore, this study evaluates the effects of silibinin onoxidative stress indices and miR-10b expression in theanimal models of breast cancer.Material and methods: In this study, 48 Balb/c mice weredivided into six groups (each group contains eight mice): thehealthy control, the cancer control, the healthy groupreceiving 20mg of silibinin, the cancer group receiving 20 mgof silibinin, the cancer group receiving 40mg of silibinin andthe cancer group receiving 80mg of silibinin for three weeks.In order to induce cancer, 4T1 cell line was used. Afterobtaining breast tumor samples, the levels of Malondialdehyde(MDA), superoxide dismutase (SOD), glutathioneperoxidase (GPX) and miR-10b expression in breast tumorbiopsy were evaluated. Data were analyzed using one-wayANOVA, Kruskal–Wallis, Mann–Whitney and t-test (p

Özet

Giriş ve Amaç: Oksidatif stres ve bazı artmış microRNA (miR’lerin) ekspresyonu kanserle ilişkili faktörler arasındadır. Silibininin bir anti-tümör etkisi vardır. Bu nedenle bu çalışmanın amacı, meme kanserinin hayvan modellerinde silibininin oksidatif stres indeksleri ve miR-10b ekspresyonu üzerindeki etkilerini değerlendirmektedir. Gereç ve Yöntemler: Bu çalışmada 48 Balb / c fare 6 gruba ayrıldı (her grup 8 fare içerir): sağlıklı kontrol, kanser kontrolü, 20 mg silibinin alan sağlıklı grup, 20 mg alan kanser grubu 40mg silibinin alan kanser grubu ve 3 hafta boyunca 80 mg silibinin alan kanser grubu. Kanseri indüklemek için 4T1 hücre hattı kullanıldı. Meme tümör örnekleri elde edildikten sonra, göğüs tümör biyopsisinde Malondialdehid (MDA), süperoksit dismutaz (SOD), glutatyon peroksidaz (GPX) ve miR-10b ekspresyon seviyeleri değerlendirildi. Veriler tek yönlü ANOVA, Kruskal–Wallis, Mann–Whitney ve T testi kullanılarak analiz edildi (p

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Cite This Article

SOLEİMANİ, F., SHARİFİ, R., MAHMOODİ, M., KASSAEE, S. M. (2022). The effects of silibinin on oxidative stress and microRNA-10b expression in animal models of breast cancer. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 6, 707–714. https://ajindex.com/articles/620760

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