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Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 6

Explore the activation efficiency of different ligand carriers on synNotch-based contact-dependent activation system

Wuling ZHU
Zhiyuan NİU
Guanxu CHEN
Han CHEN
Meichen YİN
Lingtong ZHİ
Pages
817–824
Publication Date
2022-11-06
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Three eGFPcarriers were incubated with 293T-synNotch, the activationefficiencies of these three eGFP carriers were evaluated byfluorescence microscopy.Results: The 293T-synNotch cell activated by Mag Ni-eGFPshows no observable red fluorescence; when MagCOOH-deGFP are incubated with 293T-synNotch, about50‰cells with red fluorescence appeared, also eGFP proteinshave not dropped off from the Mag COOH-deGFPbeads; 4T1-mGFP cells show the highest activation efficiency,about 21% synNotch cells are activated, when 4T1--mGFP and 293T-synNotch are incubated at high cell density.Conclusion: The 4T1-mGFP cells are the most effective ligandsfor synNotch receptor activation.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: synNotch receptors can get different inputsabout the outside environment into cells; however, syn-Notch system doesn’t work for soluble ligands.
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Key Findings & Data:This studyaims to explore the activation efficiency of different ligandcarriers on synNotch system.Methods: SynNotch-based contact-dependent activationsystem was constructed in 293T cells (293T-synNotch), thenthe synNotch receptor ligands eGFP proteins were providedby three different carriers: 400 nm Ni magnetic beads (MagNi-eGFP), 300 nm carboxyl modified Fe3O4 magnetic beads(Mag COOH-deGFP), and 4T1 cell (4T1-mGFP).
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Conclusion & Impact:Three eGFPcarriers were incubated with 293T-synNotch, the activationefficiencies of these three eGFP carriers were evaluated byfluorescence microscopy.Results: The 293T-synNotch cell activated by Mag Ni-eGFPshows no observable red fluorescence; when MagCOOH-deGFP are incubated with 293T-synNotch, about50‰cells with red fluorescence appeared, also eGFP proteinshave not dropped off from the Mag COOH-deGFPbeads; 4T1-mGFP cells show the highest activation efficiency,about 21% synNotch cells are activated, when 4T1--mGFP and 293T-synNotch are incubated at high cell density.Conclusion: The 4T1-mGFP cells are the most effective ligandsfor synNotch receptor activation.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: synNotch receptors can get different inputsabout the outside environment into cells; however, syn-Notch system doesn’t work for soluble ligands. This studyaims to explore the activation efficiency of different ligandcarriers on synNotch system.Methods: SynNotch-based contact-dependent activationsystem was constructed in 293T cells (293T-synNotch), thenthe synNotch receptor ligands eGFP proteins were providedby three different carriers: 400 nm Ni magnetic beads (MagNi-eGFP), 300 nm carboxyl modified Fe3O4 magnetic beads(Mag COOH-deGFP), and 4T1 cell (4T1-mGFP). Three eGFPcarriers were incubated with 293T-synNotch, the activationefficiencies of these three eGFP carriers were evaluated byfluorescence microscopy.Results: The 293T-synNotch cell activated by Mag Ni-eGFPshows no observable red fluorescence; when MagCOOH-deGFP are incubated with 293T-synNotch, about50‰cells with red fluorescence appeared, also eGFP proteinshave not dropped off from the Mag COOH-deGFPbeads; 4T1-mGFP cells show the highest activation efficiency,about 21% synNotch cells are activated, when 4T1--mGFP and 293T-synNotch are incubated at high cell density.Conclusion: The 4T1-mGFP cells are the most effective ligandsfor synNotch receptor activation.

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Cite This Article

ZHU, W., NİU, Z., CHEN, G., CHEN, H., YİN, M., ZHİ, L. (2022). Explore the activation efficiency of different ligand carriers on synNotch-based contact-dependent activation system. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 6, 817–824. https://ajindex.com/articles/620755

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