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Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 1

Investigation of effect of vitamin Dreceptor, calcium-sensing receptor andβ-catenin on cutaneous squamous cell carcinoma

D vitamini reseptörü, kalsiyuma duyarlı reseptör ve β-katenin’in skuamöz hücreli deri kanseri üzerine etkisinin araştırılması

Berrin TUĞRUL
Sevinç SÖYLEV
Peyker TEMİZ
Pages
91–98
Publication Date
2022-11-06
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further research is required to better understand the role of VDR and β-catenin together on cSCC.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Cutaneous squamous cell carcinoma (cSCC) is a malignant and invasive tumor which is originated from epidermis with a high incidence amongnon-melanoma skin cancers.
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Key Findings & Data:The difference between IHC staining density of VDR and β-catenin in the patient and the control groups were statistically signifi-cant (p = 0.021, p = 0.021, respectively), but not for CaSR (p = 0.237).
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Conclusion & Impact:Further research is required to better understand the role of VDR and β-catenin together on cSCC.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Cutaneous squamous cell carcinoma (cSCC) is a malignant and invasive tumor which is originated from epidermis with a high incidence amongnon-melanoma skin cancers. The aim of this study was to determine whether vitamin D receptor (VDR), calcium-sensing recep-tor (CaSR) and beta catenin (β-catenin) proteins have an effect on cSCC.Materials and methods: VDR, CaSR and β-catenin proteins in tissue samples of cSCC and control group were analyzed by immunohistochemistry (IHC) and Western blotting (WB) method. IHC findings were statistically evaluated.Results: IHC staining density of VDR and β-catenin were higher in cSCC tissue samples than control. The difference between IHC staining density of VDR and β-catenin in the patient and the control groups were statistically signifi-cant (p = 0.021, p = 0.021, respectively), but not for CaSR (p = 0.237). While the VDR and β-catenin staining rates obtained by the IHC method could be supported by WB results, the WB bands for CaSR could not be shown.Conclusion: The findings suggest that VDR and β-catenin may have an effect on the disease. Further research is required to better understand the role of VDR and β-catenin together on cSCC.

Özet

Amaç: Skuamöz hücreli deri kanseri (SHK), melanom dışıderi kanserleri arasında görülme sıklığı artan, epidermis- den köken alan malign, invaziv bir tümördür. Bu çalışma- nın amacı D vitamini reseptörü (VDR), kalsiyuma duyarlıreseptör (CaSR) ve beta katenin (β-katenin) proteinlerininskuamoz hücreli deri kanseri (cSCC) üzerinde etkili olupolmadığını belirlemektir. Gereç ve Yöntem: cSCC ve kontrol grubuna ait doku örnek- lerindeki VDR, CaSR ve β-katenin proteinleri immünohis- tokimya (IHC) ve Western blot (WB) yöntemi ile analizedildi. IHC bulguları istatistiksel olarak değerlendirildi. Bulgular: VDR ve β-catenin IHC boyama yoğunluğu,cSCC doku örneklerinde kontrol grubundan daha yük- sekti. Hasta ve kontrol gruplarında, VDR ve β-katenin IHCboyama yoğunluğu arasındaki fark istatistiksel olarakanlamlıydı (sırasıyla p = 0,021, p = 0,021), fakat CaSR içinanlamlı değildi (p = 0,237). IHC yöntemiyle elde edilenVDR ve β-catenin boyama oranları WB sonuçları ile des- teklenebilirken, CaSR için WB bantları elde edilememiştir. Sonuç: Bulgular, VDR ve β-katenin’in hastalık üze- rinde etkili olabileceğini düşündürmektedir. VDR ve β-katenin’in birlikte cSCC üzerindeki rolünü daha iyi anla- mak için ileri araştırmaların yapılması gerekmektedir.

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Cite This Article

TUĞRUL, B., SÖYLEV, S., TEMİZ, P. (2022). Investigation of effect of vitamin Dreceptor, calcium-sensing receptor andβ-catenin on cutaneous squamous cell carcinoma. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 1, 91–98. https://ajindex.com/articles/620746

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