AI Summary & Key Takeaways
AI InsightThe TNF-α cytokine level, which is the indicator for inflammations, was found to be higher in older mice than in young mice (p < 0.05).Conclusion: As a result, it was observed that cellular damage, disruption in water – electrolyte balance and increased inflammation that occur during the natural process of aging had caused serious and irreversible disturbances.
📌 3 Key Takeaways
Abstract
Objective: In this study, we aimed to investigate the changes in the levels of oxidative stress and antioxidant enzymes on the mandibular bone caused by the expression of aquaporin-1 and aquaporin-3 proteins.Material and method: 14 Balb/C white mice were divided into two groups of seven, based on whether they are young or old. Mandibular tissue samples were taken for biochemical and histological analysis.Results: Findings of our study has shown that, AQP-1 and AQP-3 immunoreactivity significantly decreased in mandibular bone tissues of aged mice in comparison to younger mice (p < 0.05). MDA and AOPP levels, which are the indicators of oxidative stress, increased in elderly mice and antioxidant defense system SOD enzyme activity was decreased (p < 0.05). The TNF-α cytokine level, which is the indicator for inflammations, was found to be higher in older mice than in young mice (p < 0.05).Conclusion: As a result, it was observed that cellular damage, disruption in water – electrolyte balance and increased inflammation that occur during the natural process of aging had caused serious and irreversible disturbances.
Özet
Amaç: Bu çalışmada, aquaporin-1 ve aquaporin-3 pro teinlerinin ekspresyonunun neden olduğu mandibularkemik üzerindeki oksidatif stres seviyelerindeki ve anti oksidan enzim aktivitelerindeki değişiklikleri araştırmayıamaçladık. Gereç ve Yöntem: 14 Balb/C beyaz fare genç veya yaşlıolup olmadıklarına göre yedişer iki gruba ayrıldı. Biyo kimyasal, histolojik analiz için mandibular doku örneklerialındı. Bulgular: Çalışmamızın bulguları, AQP-1 ve AQP-3 immü noreaktivitesinin, yaşlı farelerin mandibular kemik doku larında, genç farelere kıyasla anlamlı olarak azaldığınıgöstermiştir (p < 0.05). Oksidatif stresin göstergesi olanMDA ve AOPP düzeyleri yaşlı farelerde artmış ve antiok sidan savunma sistemi SOD enzim aktivitesi düşmüştür(p < 0.05). Enflamasyonun göstergesi olan TNF-α sitokinseviyesinin yaşlı farelerde genç farelere göre daha yüksekolduğu bulunmuştur. (p < 0.05). Sonuç: Doğal yaşlanma sürecinde meydana gelen hücre sel hasar, su – elektrolit dengesinde bozulma ve artmışenflamasyonun ciddi ve geri dönüşü olmayan bozukluk lara neden olduğu görülmüştür.