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Journals / Türk Biyokimya Dergisi / 2021 / Cilt: 46 - Sayı: 5

The protective effects of sinapic acid on acute renal ischemia/reperfusion injury

Sinapik asidin akut renal iskemi/reperfüzyon hasarı üzerine koruyucu etkileri

Fatih Fırat
Velid Ünsal
Engin Kolukcu
Fikret Gevrek
Pages
563–571
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The aim of this research was to investigate whether sinapic acid (SA) can alleviate oxidative damage, apoptosis, and inflammation in I/R induced renal injury.
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Key Findings & Data:Methods: A total of 24 male rats were randomly separa- ted into four groups as six rats in each group.
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Conclusion & Impact:Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: The aim of this research was to investigate whether sinapic acid (SA) can alleviate oxidative damage, apoptosis, and inflammation in I/R induced renal injury. Methods: A total of 24 male rats were randomly separa- ted into four groups as six rats in each group. Group 1 (Sham), Group 2 (I/R), Group 3 (I/R + SA, 10 mg/kg), Group 4 (I/R + SA, 20 mg/kg). In order to evaluate kidney func- tion serum BUN, Cr, and AST were measured in an autoanalyzer. SOD, GSH-Px, MDA, PC and NO oxidative stress parameters were measured with spectrophotome- tric methods and TNF-α, IL-1β, IL-6, KIM-1 and NGAL parameters were measured with the ELISA method. In addition, H&E method and immunohistochemical exa- minations were performed for histological evaluations of kidney tissue. Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p

Özet

Giriş: Bu araştırmanın amacı, I/R kaynaklı böbrek hasa- rında sinapik asidin (SA) oksidatif hasarı, apoptozu ve inflamasyonu hafifletip hafifletemeyeceğini araştırmaktır. Yöntemler: Toplam 24 erkek sıçan her grupta 6 adet sıçan olacak şekilde rastgele 4 gruba ayrıldı. Grup 1 (Sham), Grup 2 (I/R), Grup 3 (I/R + SA, 10 mg/kg), Grup 4 (I/R + SA, 20 mg/kg). Böbrek fonksiyonunu değerlendirmek için serum BUN, Cr ve AST otoanalizörde ölçüldü. SOD, GSH-Px, MDA, PC ve NO oksidatif stres parametreleri spektrofotometrik yöntemlerle, TNF-α, IL-1β, IL-6, KIM-1 ve NGAL parametreleri ELISA yöntemiyle ölçüldü. Ayrıca böbrek dokusunun histolojik değerlendirmeleri için H&E yöntemi ve immünohistokimyasal incelemeler yapıldı. Bulgular: SA, böbrek hasarı, inflamasyon, oksidatif stres, hücre ölümündeki artışı önemli ölçüde azaltır ve antiok- sidan enzim aktivitelerindeki azalmayı geri kazandırır (p

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Cite This Article

Fırat, F., Ünsal, V., Kolukcu, E., Gevrek, F. (2022). The protective effects of sinapic acid on acute renal ischemia/reperfusion injury. Türk Biyokimya Dergisi, Cilt: 46 - Sayı: 5, 563–571. https://ajindex.com/articles/620715

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