Journals / Türk Biyokimya Dergisi / 2009 / Cilt: 34 - Sayı: 2

Giberellik asit ve homobrassinolit uygulaması ile sıçan doku laktat dehidrogenaz aktivitesinde gözlenen değişiklikler

Differences in rat tissue lactate dehydrogenase activity caused by giberellic acid and homobrassinolide

Pages
57–61
Publication Date
2022-11-05
DOI
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Gibberellic acid increase in blood glucose, cholesterol contentand changes in white cell count were noted.Conclusion: Dietary gibberellic acid and homobrassinolide therefore affected cellular energy production, blood glucose and cholesterol content while homobrassinolide affected protein synthesis and hemoglobin content.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Lactate dehydrogenase enzyme, a tissue marker for cardiac disorders,reversibly forms pyruvate from lactate in all animal tissues.
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Key Findings & Data:Low-dose effect ofdietary plant hormones homobrassinolide and gibberellic acid on this enzyme activity was therefore investigated in normal rat tissues.Methods: Hormones were administered intradermally to male albino wistar ratgroups (100-120 g) at 10, 50 and 250 µg, in independent trials and the lactatedehydrogenase enzyme activity was assayed two hours later.Results: Homobrassinolide at 10 μg increased kidney and testicular lactate dehydrogenase enzyme specific activity, but at 50 μg increased heart and testicularlactate dehydrogenase enzyme specific activity.
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Conclusion & Impact:Gibberellic acid increase in blood glucose, cholesterol contentand changes in white cell count were noted.Conclusion: Dietary gibberellic acid and homobrassinolide therefore affected cellular energy production, blood glucose and cholesterol content while homobrassinolide affected protein synthesis and hemoglobin content.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Lactate dehydrogenase enzyme, a tissue marker for cardiac disorders,reversibly forms pyruvate from lactate in all animal tissues. Low-dose effect ofdietary plant hormones homobrassinolide and gibberellic acid on this enzyme activity was therefore investigated in normal rat tissues.Methods: Hormones were administered intradermally to male albino wistar ratgroups (100-120 g) at 10, 50 and 250 µg, in independent trials and the lactatedehydrogenase enzyme activity was assayed two hours later.Results: Homobrassinolide at 10 μg increased kidney and testicular lactate dehydrogenase enzyme specific activity, but at 50 μg increased heart and testicularlactate dehydrogenase enzyme specific activity. The homobrassinolide responseswere however below alcohol control level for all other tissues. Homobrassinolideincreased hemoglobin and total protein, decreased blood glucose and altered totalcholesterol content. Gibberellic acid increase in blood glucose, cholesterol contentand changes in white cell count were noted.Conclusion: Dietary gibberellic acid and homobrassinolide therefore affected cellular energy production, blood glucose and cholesterol content while homobrassinolide affected protein synthesis and hemoglobin content.

Özet

Amaç: Laktat dehidrogenaz tüm hayvan dokularında piruvattan laktat oluşumunugerçekleştiren enzim olup kalp rahatsızlıkları için belirteç olarak kullanılır. Çalışmada diyetle alınan düşük dozdabitki hormonları homobrassinolit ve giberellikasidin bu enzime etkileri incelenmiştir.Gereç ve yöntemler: Hormonlar bağımsız çalışmalarda erkek albino wistar sıçangruplarına 10, 50 ve 250 µg dozlarında deri içine verilmiş ve uygulamadan iki saatsonra laktat dehidrogenaz aktivitesi ölçülmüştür. Bulgular: 10 µg homobrassinolit böbrek ve testiküler laktat dehidrogenaz enziminin spesifik aktivitesini arttırırken 50 µg homobrassinolit uygulaması kalp vetestiküler laktat dehidrogenaz enziminin spesifik aktivitesinin artmasına neden olmuştur. Homobrassinolit yanıtları diğer tüm dokularda kontrol alkol seviyelerindedaha düşük bulunmuştur. Homobrassinolit hemoglobin ve total protein miktarınıarttırıken kan glukoz seviyesi düşmüş ve total kolesterol içeriği değişmiştir. Giberellik asit uygulaması ile kan glukozu, kolesterol içeriği ve beyaz küre sayısındadeğişiklikler gözlenmiştir. Sonuçlar: Diyet ile alınan giberellik asit ve homobrassinolit hücrenin enerji üretimini, kan glukoz ve kolesterol içeriğini etkiler iken homobrassilonit protein sentezive hemoglobin içeriğini değiştirir.

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Cite This Article

VIKRAMATHITHAN, J., GAUTAMI, G., SRIKUMAR, K., GANESH, I. (2022). Giberellik asit ve homobrassinolit uygulaması ile sıçan doku laktat dehidrogenaz aktivitesinde gözlenen değişiklikler. Türk Biyokimya Dergisi, Cilt: 34 - Sayı: 2, 57–61. https://ajindex.com/articles/618409

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