SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2016 / Cilt: 40 - Sayı: 2

Comparison of the development of mouse embryos manipulatedwith different biopsy techniques

Hakan SAĞIRKAYA
Ali Cihan TAŞKIN
Tolga AKKOÇ
Haydar BAĞIŞ
Sezen ARAT
Pages
157–162
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P< 0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine.
📊
Key Findings & Data:The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques.
💡
Conclusion & Impact:At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P< 0.05).
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P< 0.05).

🔖

Cite This Article

SAĞIRKAYA, H., TAŞKIN, A. C., AKKOÇ, T., BAĞIŞ, H., ARAT, S. (2022). Comparison of the development of mouse embryos manipulatedwith different biopsy techniques. Turkish Journal of Veterinary and Animal Sciences, Cilt: 40 - Sayı: 2, 157–162. https://ajindex.com/articles/615140

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Handyside AH, Delhanty JDA. Preimplantation genetic diagnosis: strategies and surprises. Trends Genet 1997; 13: 270–275
  2. Tarkowski AK. Experiments on the development of isolated blastomeres of mouse eggs. Nature 1959; 184: 1286–1287
  3. Gardner RL, Edwards RG. Control of the sex ratio at full term in the rabbit by transferring sexed blastocysts. Nature 1968; 218: 346–348
  4. Willadsen SM. The viability of early cleavage stages containing half the normal number of blastomeres in the sheep. J Reprod Fertil 1980; 59: 57–62
  5. Santalo ET, Grossmann M, Egozcue J. Does Ca2+/Mg2+-free medium have an effect on the survival of the preimplantation mouse embryo after biopsy? Hum Reprod Update 1996; 2: 257–261
  6. Illmensee K, Kaskar K, Zavos P. In-vitro blastocyst development from serially split mouse embryos and future implications for human ART. Fertil Steril 2006; 86: 1112–1120
  7. Mitalipov SM, Yeoman RR, Kuo HC, Wolf DP. Monozygotic twinning in rhesus monkeys by manipulation of in vitro-derived embryos. Biol Reprod 2002; 66: 1449–1455
  8. Peippo J, Viitala S, Virta J, Räty M, Tammiranta N, Lamminen T, Vilkki J. Birth of correctly genotyped calves after multiplex marker detection from bovine embryo microblade biopsies. Mol Reprod Dev 2007; 74: 1373–1378
  9. Wang Z, Trounson A, Dziadek M. Developmental capacity of mechanically bisected mouse morulae and blastocysts. Reprod Fert Develop 1990; 2: 683–691
  10. Nagashima H, Matsui K, Sawasaki T, Kano Y. Production of monozygotic mouse twins from microsurgically bisected morulae. Reprod Fert Develop 1984; 70: 357–362
  11. Cenariu M, Pall E, Cernea C, Groza I. Evaluation of bovine embryo biopsy techniques according to their ability to preserve embryo viability. J Biomed Biotechnol 2012; 2012: 541384
  12. Bredbacka P, Kankaanpää A, Peippo J. PCR-sexing of bovine embryos: a simplified protocol. Theriogenology 1995; 44: 167–176
  13. Bagis H, Akkoc T, Taskin C, Arat S. Comparison of different cryopreservation techniques: higher survival and implantation rate of frozen-thawed mouse pronuclear embryos in the presence of beta-mercaptoethanol in post-thaw culture. Reprod Domest Anim 2010; 45: 332–337
  14. Krzyminska UB, Lutjen JO, Neil C. Assessment of the viability and pregnancy potential of mouse embryos biopsied at different preimplantation stages of development. Hum Reprod 1990; 5: 203–208
  15. Parks JC, McCallie BR, Janesch AM, Schoolcraft WB, Katz-Jaffe MG. Blastocyst gene expression correlates with implantation po