SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2021 / Cilt: 45 - Sayı: 1

The prevalence of seven crucial honeybee viruses using multiplex RT-PCR and their phylogenetic analysis

Abdurrahman Anıl ÇAĞIRGAN
Zafer YAZICI
Pages
44–55
Publication Date
2022-11-06
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed the virus with the highest prevalence was DWV followed by BQCV, ABPV, SBV, and CBPV.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Abstract: Honey bee viruses may be of severe adverse effect on bee colonies.
📊
Key Findings & Data:A total of 111 apiaries were randomly selected and adult bees and larvae samples were obtained from seemingly healthy colonies located at Aegean region of west Turkey.
💡
Conclusion & Impact:The results showed the virus with the highest prevalence was DWV followed by BQCV, ABPV, SBV, and CBPV.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Abstract: Honey bee viruses may be of severe adverse effect on bee colonies. Till now, more than twenty honey bee viruses have been identified. The aim of this study was to investigate the prevalence of seven honey bee viruses, namely Israeli acute paralysis virus (IAPV), deformed wing virus (DWV), sacbrood virus (SBV), acute bee paralysis virus (ABPV), black queen cell virus (BQCV), Kashmir bee virus (KBV), and chronic bee paralysis virus (CBPV) using a multiplex reverse transcription polymerase chain reaction (mRT-PCR). A total of 111 apiaries were randomly selected and adult bees and larvae samples were obtained from seemingly healthy colonies located at Aegean region of west Turkey. The presence of black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), acute bee paralysis virus (ABPV), and sacbrood virus (SBV) was detected, while Israeli acute paralysis virus (IAPV) or Kashmir bee virus (KBV) couldn’t be detected in studied colonies. The results showed the virus with the highest prevalence was DWV followed by BQCV, ABPV, SBV, and CBPV. PCR products for DWV, BQCV, ABPV, SBV, CBPV were sequenced and compared to the Genbank database. The Turkish strains demonstrated heterogeneous populations of DWV, BQCV, and relatively homogenous populations of ABPV, CBPV, and SBV. Based on these results, SBV and CBPV had formed different branch and genotypes when compared to previous studies. In conclusion, this study provides information about the distinctive honey bee viruses for future researches in Turkey, which holds the largest number of managed colonies in Europe. Key words: Apis mellifera, deformed wing virus, black quenn cell virus, sacbrood virus, chronic bee paralysis virus, acute bee paralysis virus

🔖

Cite This Article

ÇAĞIRGAN, A. A., YAZICI, Z. (2022). The prevalence of seven crucial honeybee viruses using multiplex RT-PCR and their phylogenetic analysis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 45 - Sayı: 1, 44–55. https://ajindex.com/articles/615081

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Doğanay A, Girişgin AO. Bal arisi yetiştiriciliği ürünleri hastaliklari. In: Doganay A, Aydin L (editors). 1st ed. Bursa: Dora Basın Yayın Dağıtım; 2017.pp. 21-146
  2. Remnant EJ, Shi M, Buchmann G, Blacquiere T, Holmes EC, et al. A diverse range of novel RNA viruses in geographically distinct honey bee populations. Journal of Virology 2017; 91: e00158-7. doi: 10.1128/JVI.00158-17
  3. Levin S, Galbraith D, Sela N, Erez T, Grozinger CM, et al. Presence of Apis Rhabdovirus-1 in populations of pollinators and their parasites from two continents. Frontiers in Microbiology 2017; 12; 8:2482. doi: 10.3389/fmicb.2017.02482
  4. Levin S, Sela N, Erez T, Nestel D, Pettis J, et al. New viruses from the ectoparasite mite Varroa destructor infesting Apis mellifera and Apis cerena. Viruses 2019; 11: 94. doi:10.3390/ v11010094
  5. Bailey L, Ball BV. Honey Bee Pathology. 2 th edition. London, UK: Academic Press; 1991. 6. de Miranda JR. Diagnostic techniques for virus detection in honey bees. In: Aubert MFA, Ball BV, Fries I, Morritz RFA, Milani N, Bernardinelli I (editors). Virology and the Honey Bee. Luxembourg: EEC Publications; 2008. pp. 121-232
  6. Ribière M, Ball BV, Aubert MFA. Natural history and geographic distribution of honey bee viruses. In: Aubert MFA, Ball BV, Fries I, Morritz RFA, Milani N, Bernardinelli I (editors). Virology and the Honey Bee. Luxembourg: EEC Publications, 2008. pp. 15-84
  7. Tantillo G, Bottaro M, Di Pinto A, Martella V, Di Pinto P et al. Virus infections of honeybees Apis mellifera. Italian Journal of Food Safety 2005; 30: 4 (3): 5364
  8. McMenamin AJ, Flenniken ML. Recently identified bee viruses and their impact on bee pollinators. Current Opinion in Insect Science. 2018; 26: 120-129
  9. Valles SM, Chen YP, Firth AE, Guerin DMA, Hashimoto Y, et al. ICTV virus taxonomy profile: Iflaviridae. Journal of General Virology 2017; 98: 527–528
  10. Valles SM, Chen YP, Firth AE, Guerin DMA, Hashimoto Y, et al. ICTV virus taxonomy profile: Dicistroviridae. Journal of General Virology 2017; 98: 355–356
  11. Ball BV. The damaging effects of Varroa jacobsoni. In: Matheson A (editor). Living with Varroa, International Bee Research Association. Cardiff, UK: IBRA Publication; 1993. pp. 9-16
  12. Chen YP, Smith B, Collins AM, Pettis JS, Feldlaufer MF. Detection of deformed wing virus infection in honey bees, Apis mellifera L., in the United States. American Bee Journal 2004; 144: 557-559
  13. Chen YP, Siede R. Honey bee viruses. Advances in Virus Rese