Journals / Eurasian Journal of Veterinary Sciences / 2020 / Cilt: 36 - Sayı: 2

The investigation of Israil acute bee paralysis virus, sacbrood virus, kashmir bee virus and chronic bee paralysis virus in honeybees (Apis mellifera)

Bal arılarında (Apis mellifera) İsrail akut arı felci virusu, sacbrood virusu, kaşmir arı felci virusu ve kronik arı felci virusunun araştırılması

Pages
96–101
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Besides, the futurestudies on honeybee viruses should be planned to include wider areas andpopulations and to reveal viral phylogenies

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This study aims to investigate the presence and prevalence ofIsrael acutebee paralysis virus (IAPV), Sacbrood virus (SBV), Kashmir bee virus (KBV) andChronic bee paralysis virus (CBPV) by Reverz Transcriptase-Polymerase ChainReaction (RT-PCR) method in apiaries around Burdur region.Materials and Methods: During the study, 30 beehives from 15 apiaries wereselected randomly and sampling procedures were carried out. 30 adult beeswere collected from each of the sampled hives to form a pool.
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Key Findings & Data:After the samp-les were homogenized, total nucleic acid extraction was performed and thenviral nucleic acid was searched by RT-PCR method using specific primers.Results: In this study, in consequence of analyzing 30 samples from 15 api-aries by RT-PCR method, SBV was detected in 10 samples obtained from 5apiaries (33.3%) and CBPV was found in 4 samples from 2 apiaries (13.3.%).IAPV and KBV were not detected.
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Conclusion & Impact:Besides, the futurestudies on honeybee viruses should be planned to include wider areas andpopulations and to reveal viral phylogenies
🔬Design:Lab & Field Experiment
📍Scope:30 samples
⚙️Variables:Domain Parameters

Abstract

Aim: This study aims to investigate the presence and prevalence ofIsrael acutebee paralysis virus (IAPV), Sacbrood virus (SBV), Kashmir bee virus (KBV) andChronic bee paralysis virus (CBPV) by Reverz Transcriptase-Polymerase ChainReaction (RT-PCR) method in apiaries around Burdur region.Materials and Methods: During the study, 30 beehives from 15 apiaries wereselected randomly and sampling procedures were carried out. 30 adult beeswere collected from each of the sampled hives to form a pool. After the samp-les were homogenized, total nucleic acid extraction was performed and thenviral nucleic acid was searched by RT-PCR method using specific primers.Results: In this study, in consequence of analyzing 30 samples from 15 api-aries by RT-PCR method, SBV was detected in 10 samples obtained from 5apiaries (33.3%) and CBPV was found in 4 samples from 2 apiaries (13.3.%).IAPV and KBV were not detected. As a result of evaluations, 4 samples from 2apiaries were considered to have been infected with both SBV and CBPV, whichmeans a mixed infection occurred in these hives.Conclusion: It is clear that the so-called diseases were regarded important interms of beekeeping in not only Burdur region but also in the other regions ofTurkey and serious precautions should be taken to prevent infections. Strugg-ling with bee parasites that play the key role in transmission viral agents andenhancing the immune resistance ofbees and obeying the rules ofhygiene andsanitation should not be ignored in protecting bee health. Besides, the futurestudies on honeybee viruses should be planned to include wider areas andpopulations and to reveal viral phylogenies

Özet

Amaç: Bu araştırmada, Burdur yöresinde halk elinde bulunan kovanlıklarda İsrail akut arı felci virusu (IAPV), Sacbrood virus (SBV), Kaşmir arı felci virusu ve Kronik arı felci virusunun (CBPV) Reverz Transcriptase-Polymerase Cha- in Reaction (RT-PCR) yöntemiyle varlıklarının/yaygınlıklarının araştırılması amaçlanmıştır. Gereç ve Yöntem: Araştırmada, 15 arıcılık işletmesinden 30 kovan rastgele seçilerek örnekleme yapılmıştır. Numune alınan kovanların her birinden 30 ergin arı toplanarak, havuz oluşturulmuştur. Örnekler homojenize edildikten sonra total nükleik asit ekstraksiyonu yapılmış, ardından spesifik primerler kullanılarak RT-PCR yöntemiyle söz konusu viruslara ait nükleik asit varlıkları araştırıldı. Bulgular: Araştırmamızda 15 arıcılık işletmesinden alınan 30 örneğin RT-PCR yöntemi ile analiz edilmesi sonucunda 5 arıcılık işletmesinden alınan 10 nu- munede (%33.3) SBV, iki arıcılık işletmesinden alınan 4 numunede (%13.3) ise KBPV tespit edilmiştir. IAPV ve KBV hiçbir kovanlıkta tespit edilmemiştir. Yapılan değerlendirmelerde 2 işletmeden alınan 4 örneğin hem SBV hem de CBPV ile enfekte olduğu, yani bu kovanlarda miksenfeksiyonun bulunduğu belirlenmiştir. Öneri: Söz konusu hastalıkların hem Burdur bölgesinde hem de Türkiye’nin diğer bölgelerinde arı yetiştiriciliği bakımından önemli olduğu ve enfeksiyon- ların önlenmesi amacı ile ciddi tedbirlerin alınması gerektiği görülmektedir. Arılarda birçok viral etkenin yayılmasında kilit rol oynayan Varroa ile müca- dele edilmeli, arı immun direnci arttırılmalı ve viral etkenlerin bulaşmasına engel olmak için gerekli hijyen ve sanitasyon kurallarına uyulmalıdır. Ayrıca bundan sonra yapılacak bal arısı virusları ile alakalı çalışmaların daha geniş alanları ve popülasyonları kapsayacak şekilde ve viral filogenilerin ortaya ko- nulacak şekilde planlanmasının faydalı olacağı kanısına varılmıştır.

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Cite This Article

ÇAĞIRGAN, A. A., YILDIRIM, Y., USTA, A. (2022). The investigation of Israil acute bee paralysis virus, sacbrood virus, kashmir bee virus and chronic bee paralysis virus in honeybees (Apis mellifera). Eurasian Journal of Veterinary Sciences, Cilt: 36 - Sayı: 2, 96–101. https://ajindex.com/articles/509811

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