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Journals / Turkish Journal of Veterinary and Animal Sciences / 2019 / Cilt: 43 - Sayı: 1

Phylogrouping and antimicrobial resistance analysis of extraintestinal pathogenic Escherichia coli isolated from poultry species

Ranjan MAHADEVASWAMY
Samir JADHAV
Chandrakant BHONG
Dattatray GANDHALE
Rahul KOLHE
Saba NALBAND
Sujata BHAVE
Dushyant MUGLIKAR
Pages
117–126
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Poultry and raw chicken harbor multidrug-resistant, ESBL-type E. coli as well as ExPECand these strains may be transmitted to humans via the food chain.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenicEscherichia coli (ExPEC) in the feces of healthy poultry and retail chicken.
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Key Findings & Data:All 146 E. coli recovered from 351 samples were screened byPCR for detection of ExPEC strains.
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Conclusion & Impact:Poultry and raw chicken harbor multidrug-resistant, ESBL-type E. coli as well as ExPECand these strains may be transmitted to humans via the food chain.
🔬Design:Research Article
📍Scope:351 samples
⚙️Variables:Domain Parameters

Abstract

This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenicEscherichia coli (ExPEC) in the feces of healthy poultry and retail chicken. All 146 E. coli recovered from 351 samples were screened byPCR for detection of ExPEC strains. Nineteen (13.01%) isolates were confirmed as ExPEC. Distribution of ExPEC strains was revealedas follows: broiler (25%), layer (15.87%), and raw chicken (3.12%). Turkey, duck, and water samples were negative for ExPEC strains.ExPEC strains belonged to phylogenetic groups B2 (52.63%), A (36.84%), and D (10.53%). Twenty-three (15.75%) isolates were ESBLpositive,including four ExPEC strains. ESBL-positive E. coli were isolated from all the samples except turkey. A high degree of resistanceto commonly used antimicrobials, namely nalidixic acid (95.89%), tetracycline (95.89%), trimethoprim (89.04%), colistin (82.88%), andciprofloxacin (54.11%), including β-lactam antimicrobials ampicillin (84.93%) and amoxicillin/clavulanic acid (81.51%), was expressedby the isolates. Out of fifteen randomly selected ESBL-positive E. coli isolates, β-lactam genes, namely blaTEM, blaCTX-M, and blaOXA,were detected in three, six, and one, respectively. Poultry and raw chicken harbor multidrug-resistant, ESBL-type E. coli as well as ExPECand these strains may be transmitted to humans via the food chain.

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Cite This Article

MAHADEVASWAMY, R., JADHAV, S., BHONG, C., GANDHALE, D., KOLHE, R., NALBAND, S., BHAVE, S., MUGLIKAR, D. (2022). Phylogrouping and antimicrobial resistance analysis of extraintestinal pathogenic Escherichia coli isolated from poultry species. Turkish Journal of Veterinary and Animal Sciences, Cilt: 43 - Sayı: 1, 117–126. https://ajindex.com/articles/614899

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