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Journals / Turkish Journal of Veterinary and Animal Sciences / 2019 / Cilt: 43 - Sayı: 3

Comparative detection of bovine herpesvirus-1 using antigen ELISA, immunohistochemistry and immunofluorescence methods in cattle with pneumonia

Mehmet Özkan TİMURKAN
Yavuz Selim SaĞLAM
Selim ÇOMAKLI
Pages
306–313
Publication Date
2022-11-04
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In conclusion, it has been observed that when IF method is not available, IHC is more reliable thanantigen ELISA in the diagnosis of BoHV-1.

📌 3 Key Takeaways

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Objective & Hypothesis:This study was conducted to detect the prevalence and presence of bovine herpesvirus-1 (BoHV-1) using immunohistochemistry(IHC), immunofluorescence (IF) and antigen enzyme-linked immunosorbent assay (ELISA) in the lung samples of cattle (n = 1023).
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Key Findings & Data:Inaddition, three methods were compared using receiver operating characteristic (ROC) and chi-square test (χ2 test), and their usabilitywere evaluated in laboratory conditions.
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Conclusion & Impact:In conclusion, it has been observed that when IF method is not available, IHC is more reliable thanantigen ELISA in the diagnosis of BoHV-1.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This study was conducted to detect the prevalence and presence of bovine herpesvirus-1 (BoHV-1) using immunohistochemistry(IHC), immunofluorescence (IF) and antigen enzyme-linked immunosorbent assay (ELISA) in the lung samples of cattle (n = 1023). Inaddition, three methods were compared using receiver operating characteristic (ROC) and chi-square test (χ2 test), and their usabilitywere evaluated in laboratory conditions. Macroscopically, pneumonia was seen in 120 of the lung tissue samples (11.73%). Basedon the microscopic examinations, the pneumonia types were classified as catarrhal-suppurative bronchopneumonia (6.7%), fibrinousbronchopneumonia (5%), interstitial pneumonia (84.1%), and granulomatous pneumonia (4.2%). The IHC, IF and antigen ELISApositivity were 48.33%, 50.83%, and 29.9%, respectively. Considering IF as a gold standard, IHC had more acceptable sensitivity andspecificity than antigen ELISA. In conclusion, it has been observed that when IF method is not available, IHC is more reliable thanantigen ELISA in the diagnosis of BoHV-1.

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Cite This Article

TİMURKAN, M. Ö., SaĞLAM, Y. S., ÇOMAKLI, S. (2022). Comparative detection of bovine herpesvirus-1 using antigen ELISA, immunohistochemistry and immunofluorescence methods in cattle with pneumonia. Turkish Journal of Veterinary and Animal Sciences, Cilt: 43 - Sayı: 3, 306–313. https://ajindex.com/articles/614880

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