Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2015 / Cilt: 21 - Sayı: 1

The molecular characterization and detection of Group A rotavirus from calves with diarrhea in Turkish republic of northern cyprus

Kuzey Kıbrıs Türk Cumhuriyeti'nde ishalli buzağılarda Grup A rotavirus tespiti ve moleküler karakterizasyonu

Pages
127–130
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Findings revealed that the rotavirus circulating within this farm belongs to G6 and P[11] genotype.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the molecular characterization of the Group A rotavirus obtained from calves with diarrhea in a farm in TRNC was reported.
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Key Findings & Data:Faces samples were detected as positive by ELISA and RT-PCR depending on the amplification of the VP6 gene of rotavirus.
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Conclusion & Impact:Findings revealed that the rotavirus circulating within this farm belongs to G6 and P[11] genotype.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the molecular characterization of the Group A rotavirus obtained from calves with diarrhea in a farm in TRNC was reported. Faces samples were detected as positive by ELISA and RT-PCR depending on the amplification of the VP6 gene of rotavirus. Then the aetiological agent was analyzed by RT-PCR with generic and type-spesific to the genome segments encoding VP4 and VP7 of rotavirus. Findings revealed that the rotavirus circulating within this farm belongs to G6 and P[11] genotype. This is the first study to report the G and P genotypes of Group A rotavirus from calves with diarrhea in TRNC. Keywords: Rotavirus, Calf, Diarrhoea, Genotype, TRNC

Özet

Bu çalışmada, Kuzey Kıbrıs Türk Cumhuriyeti’nde (KKTC) yerleşik bir sığır yetiştiriciliği işletmesinde bulunan ishalli buzağılarda saptanan Grup A rotavirusun moleküler karakterizasyonu bildirildi. İshalli buzağılardan sağlanan dışkı örnekleri antijen ELISA ve Grup A rotavirus VP6 proteini kodlayan gen bölgeleri esas alınarak yapılan RT-PCR ile test edildi. Daha sonra enfeksiyona neden olan virusun VP4 ve VP7 proteinlerini kodlayan gen bölgeleri, generik ve tip spesifik primerler kullanılarak çoğaltıldı. Elde edilen verilere dayanılarak söz konusu işletmede bulunan buzağılarda enfeksiyona neden olan rotavirusun G6P[11] genotipe sahip olduğu belirlendi. Bu çalışma, KKTC’de buzağılarda Grup A rotavirusların G ve P genotiplerinin bildirildiği ilk çalışmadır.

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Cite This Article

ALKAN, F., TİMURKAN, M. Ö., KARAYEL, İ. (2015). The molecular characterization and detection of Group A rotavirus from calves with diarrhea in Turkish republic of northern cyprus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 21 - Sayı: 1, 127–130. https://ajindex.com/articles/272590

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References (10)

  1. Makoschey B, Klee W, Martella V, Bridger J, Smiths DG, Daugschies A, Millemann Y, Liebler-Tenorio E, Snodgrass D, Claerebout E, Bendali F, van de Ven J, Garcia A, Illek J, Kaske M, Cutler K, Gonzalez- Martin JV, Carvalho LM, Crouch C, Thiry E: Neonatal health in calves- comprehensive solutions for complex enteric disorders. Berl Munch Tierarztl Wochenschr, 122, 398-408, 2009
  2. Estes MK, Kapikian AZ: Rotaviruses. In, Knipe DM, Howley PM (Eds): Field’s Virology. 1917-1974, Williams and Williams, Philadelphia, 2007
  3. Matthıjnssens J, Ciarlet M, Mcdonald SM, Attoui H, Banyai K, Brister JR, Buesa J, Esona MD, Estes MK, Gentsch JR, Iturrıza-Gomara M, Johne R, Kirkwood CD, Martella V, Mertens PP, Nakagomi O,Parreno V, Rahman M, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Patton JT, Desselberger U, Vanranst M: Uniformity of rotavirus strain nomencuature proposed bythe Rotavirus Classification Working Group (RCWG). Arch Virol, 156, 1397-1413, 2011
  4. Alkan F, Timurkan MÖ, Karayel İ: Rotavirus diarrhea outbreaks in Arabian thoroughbred foals in a stud farm, Turkey. Kafkas Vet Fak Derg, 19 (Suppl-A), A141-A145, 2013
  5. Alkan F, Özkul A, Oğuzoğlu TC, Timurkan MÖ, Çalışkan E, Martella V, Burgu İ: Distribution of G (VP7) and P (VP4) Genotypes of Group A bovine rotaviruses from Turkish calves with diarrhea, 1997-2008. Vet Microbiol, 141, 231-237, 2010
  6. Garaicoechea L, Bok K, Jones LR, Combessies G, Odeon A, Fernandez F, Parreno V: Molecular characterization of bovine rotavirus circulating in beef and dairy herds in Argentina during a 10-year period (1994-2003). Vet Microbiol, 118, 1-11, 2006
  7. Falcone E, Tarantino M, Di Trani L, Cordioli P, Lavazza A, Tollis M: Determination of bovine rotavirus G and P serotypes in Italy by PCR. J Clin Microbiol, 37, 3879-3882, 1999
  8. Fodha I, Boumaiza A, Chouikha A, Dewar J, Armah G, Geyer A, Trabelsi A, Steele AD: Detection of group A rotavirus strains circulating in calves in Tunisia. J Vet Med B Infect Dis Vet Public Health, 52 (1): 49-50, 2005
  9. Badaracco A, Garaicoechea L, Matthijnssens J, Louge Uriarte Odeon A, Bilbao G, Fernandez F, Parra GI, Parreno V: Phylogenetic analyses of typical bovine rotavirus genotypes G6, G10, P[5] and P[11] circulating in Argentinean beef and dairy herds. Infection, Genetics and Evolution, 18, 18-30, 2013
  10. Abe M, Ito N, Masatani T, Nakagawa K, Yamaoka S, Kanamaru Y, Suzuki H, Shibano K, Arashi Y, Sugiyama M: Whole genom characterisation of new bovine rotavirus G21P[29]