AI Summary & Key Takeaways
AI InsightThese findings will be beneficial in future comprehensive studies on the miRNAs involved in regulating the development of follicular and luteal tissues in cows.
📌 3 Key Takeaways
Abstract
MicroRNAs (miRNAs) regulate many physiological pathways, including development, cell differentiation, immune response as well as diseases by post-transcriptional processes. However, information is limited regarding the biological roles of miRNAs in the development of the follicular and luteal tissues in cows. The main aim of the present study was to identify miRNAs that are expressed in the follicular and luteal tissues of cows. We used a comparative RNA sequencing method to identify miRNA candidates that might play key roles in the follicular and luteal phases of Holstein cows. Nine miRNAs were expressed at a high ratio: 8 in the follicular tissue and one in the luteal tissue. Bioinformatics analysis predicted 2479 target genes. RNA sequencing identified several miRNA candidates, including bta-miR-196a, bta-miR-490, bta-miR-1247-5p, bta-miR-34c, and bta-miR-222. These were associated with pathways like TGF-beta signaling, gonadotropin-releasing hormone receptor, prolactin signaling, and progesterone-mediated oocyte maturation associated with the reproductive system. These findings will be beneficial in future comprehensive studies on the miRNAs involved in regulating the development of follicular and luteal tissues in cows.