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Journals / Turkish Journal of Pharmaceutical Sciences / 2020 / Cilt: 17 - Sayı: 5

Determination of the Genetic Relationships Among Salvia Species by RAPD and ISSR Analyses

Salvia Türleri Arasındaki Genetik İlişkilerin RAPD ve ISSR Analizleriyle Belirlenmesi

Mustafa KORKMAZ
Serap SUNAR
Güleray AĞAR
Burcu SIĞMAZ
Pages
480–485
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of the combined analysis, it was shown that similarities between the species varied between 0.54 (S. rosifolia-S. sclarea, S. rosifolia-S. limbata, and S. staminea-S. verticillata) and 0.93 (S. sclera-S. divaricata).Conclusion: The findings show that the two markers represent powerful instruments for assessing the genetic diversity and relationships among Salvia species.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives:Salvia L. is the largest genus of the family Lamiaceae, which includes approximately 1000 species.
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Key Findings & Data:According to recent studies, 100 Salvia species in total grow in Turkey.
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Conclusion & Impact:As a result of the combined analysis, it was shown that similarities between the species varied between 0.54 (S. rosifolia-S. sclarea, S. rosifolia-S. limbata, and S. staminea-S. verticillata) and 0.93 (S. sclera-S. divaricata).Conclusion: The findings show that the two markers represent powerful instruments for assessing the genetic diversity and relationships among Salvia species.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives:Salvia L. is the largest genus of the family Lamiaceae, which includes approximately 1000 species. According to recent studies, 100 Salvia species in total grow in Turkey. At the same time, 53% of them are endemic. The purpose of this study was to investigate the genetic relationships among 15 Salvia species that grow in wild conditions in Turkey’s Eastern Anatolia region.Materials and Methods: The genetic relationships among 15 Salvia species were investigated using inter-simple sequence repeat (ISSR) and random amplified polymorphic-DNA (RAPD) profiles in the present study. Thirteen ISSR primers and 11 RAPD primers were utilized. The ISSR and RAPD data were combined to construct the unweighted pair group method using arithmetic average cluster.Results: Based on the RAPD and ISSR data, the Salvia species were classified into six groups. As a result of the combined analysis, it was shown that similarities between the species varied between 0.54 (S. rosifolia-S. sclarea, S. rosifolia-S. limbata, and S. staminea-S. verticillata) and 0.93 (S. sclera-S. divaricata).Conclusion: The findings show that the two markers represent powerful instruments for assessing the genetic diversity and relationships among Salvia species.

Özet

Amaç:Salvia L., yaklaşık 1000 tür içeren Lamiaceae familyasının en büyük cinsidir. Son çalışmalara göre, Türkiye’de toplam 100 Salvia türü yetişmektedir. Aynı zamanda, türlerin %53’ü endemiktir. Bu çalışmada, Türkiye’nin Doğu Anadolu Bölgesi’nde doğal olarak yetişen 15 Salvia türü arasındaki genetik çeşitliliğin değerlendirilmesi amaçlanmıştır.Gereç ve Yöntemler: On beş Salvia türü arasındaki genetik çeşitlilik, random amplifiye polimorfik DNA (RAPD) ve ISSR DNA profilleri kullanılarak araştırıldı. On üç ISSR primeri ve on bir RAPD primeri kullanıldı. Aritmetik ortalamayı kullanan ağırlıksız çift grup metodu kümelenmesi ISSR ve RAPD verilerinin kombinasyonu ile gerçekleştirildi.Bulgular: RAPD ve ISSR verilerinin sonuçlarına göre, Salvia türleri altı gruba ayrılmıştır. Kombine analiz sonucunda türlerin benzerliklerinin 0,54 (S. rosifolia-S. sclarea, S. rosifolia-S.limbata ve S. staminea-S. verticillata) ve 0,93 (S. sclera-S. divaricata) arasında değiştiği görülmüştür. Sonuç: Bu çalışmada, hem RAPD hem de ISSR belirteçlerinin Salvia türleri arasındaki genetik çeşitliliği incelemek için kullanılabileceği gösterilmiştir.

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Cite This Article

KORKMAZ, M., SUNAR, S., AĞAR, G., SIĞMAZ, B. (2022). Determination of the Genetic Relationships Among Salvia Species by RAPD and ISSR Analyses. Turkish Journal of Pharmaceutical Sciences, Cilt: 17 - Sayı: 5, 480–485. https://ajindex.com/articles/613729

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