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Journals / Turkish Journal of Pharmaceutical Sciences / 2021 / Cilt: 18 - Sayı: 1

Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle

Çiğneme Fonksiyonel Yükü Fare Masseter Kasında ACTN2 ve ACTN3’ün mRNA Ekspresyon Düzeylerini ve α-Aktin-2’nin Protein Ekspresyonunu Artırır

Nur MASITA SILVIANA
Sri ANDARINI
Diana LYRAWATI
Mohammad HIDAYAT
Pages
28–33
Publication Date
2022-11-02
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Key words: α-actinins, masseter muscles, masticatory function

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: α-actinins play structural and regulatory roles in cytoskeletal organization.
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Key Findings & Data:They form a lattice structure that secures actin in thin filaments, which generate and transmit muscle contractile forces.
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Conclusion & Impact:Key words: α-actinins, masseter muscles, masticatory function
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: α-actinins play structural and regulatory roles in cytoskeletal organization. They form a lattice structure that secures actin in thin filaments, which generate and transmit muscle contractile forces. The morphological and biochemical characteristics of rat masseter muscles are known to change reactions to masticatory functional loads, but their effect on α-actinins remains unknown. This study aimed to determine the response of α-actinins to masticatory functional loads. Materials and Methods: Twenty-four male Wistar rats aged 3 weeks were divided randomly into 3 groups of liquid diet (LD), soft diet, and hard diet (HD). The rats were then sacrificed at the end of 8 weeks. The middle part of superficial masseter muscles was examined to investigate the masticatory effect of functional load on the mRNA expression levels of ACTN2 and ACTN3 and the protein expression levels of α-actinin-2 and α-actinin-3. Results: The mRNA expression levels of ACTN2 and ACTN3 and the protein expression levels of α-actinin-2 of the HD group were significantly higher than those of the LD group, which served as the control group. Conclusion: Masticatory functional load organizes the mRNA expression levels of ACTN2 and ACTN3 and the protein expression levels of α-actinin-2 in rat masseter muscles through stimuli during muscle physiological adaptation. Key words: α-actinins, masseter muscles, masticatory function

Özet

Amaç: α-aktininler, hücre iskeleti organizasyonunda yapısal ve düzenleyici roller oynarlar. Aktin, kas kasılma kuvvetlerini üreten ve ileten ince filamentler halinde sabitleyen bir kafes yapısı oluştururlar. Sıçan masseter kaslarının morfolojik ve biyokimyasal özelliklerinin çiğneme fonksiyonel yüküne karşı verilen tepkiyi değiştirdiği bilinmektedir, ancak bunların α-aktininler üzerindeki etkisi bilinmemektedir. Bu çalışma, α-aktininlerin çiğneme fonksiyonel yüküne karşı verdiği tepkiyi belirlemeyi amaçlamıştır. Gereç ve Yöntemler: Üç haftalık 24 erkek Wistar sıçan rastgele sıvı diyet (LD), yumuşak diyet ve sert diyet (HD) uygulanan gruplar olmak üzere 3’e ayrıldı. Sıçanlar daha sonra 8 haftanın sonunda sakrifiye edildi. Yüzeyel masseter kaslarının orta kısmı, fonksiyonel yükün ACTN2 ve ACTN3’ün mRNA ekspresyon seviyeleri ve α-aktin-2 ve α-aktin-3’ün protein ekspresyon seviyeleri üzerinde çiğnemenin etkisini araştırmak için incelendi.

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Cite This Article

SILVIANA, N. M., ANDARINI, S., LYRAWATI, D., HIDAYAT, M. (2022). Masticatory Functional Load Increases the mRNA Expression Levels of ACTN2 and ACTN3 and the Protein Expression of α-Actinin-2 in Rat Masseter Muscle. Turkish Journal of Pharmaceutical Sciences, Cilt: 18 - Sayı: 1, 28–33. https://ajindex.com/articles/613647

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