Journals / Turkish Journal of Pharmaceutical Sciences / 2021 / Cilt: 18 - Sayı: 1

Antibacterial, Antifungal, and Antioxidant Activity of Cleome coluteoides: An In Vitro Comparative Study Between Leaves, Stems, and Flowers

Cleome coluteoides Boiss Antibakteriyel, Antifungal ve Antioksidan Aktiviteleri: Yaprak, Çiçek ve Kök Ekstrelerinin İn Vitro Karşılaştırmalı Çalışması

Pages
10–16
Publication Date
2022-11-02
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: Results showed that Gram-negative bacteria and fungus were resistant to various plant extracts.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Cleome coluteoides, which belongs to the Capparidaceae family, and has been used in folk medicine for a long time.
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Key Findings & Data:Antibacterial and antifungal activities were assayed by disk and well diffusion methods, and the antioxidant activity was screened by 2,2-diphenyl1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma methods.
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Conclusion & Impact:Results: Results showed that Gram-negative bacteria and fungus were resistant to various plant extracts.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Cleome coluteoides, which belongs to the Capparidaceae family, and has been used in folk medicine for a long time. Our research aims to measure the antioxidant, antibacterial, and antifungal activities of C. coluteoides. Materials and Methods: Various solvents, such as ethyl acetate, methanol, and dichloromethane, were used to extract different plant parts. Antibacterial and antifungal activities were assayed by disk and well diffusion methods, and the antioxidant activity was screened by 2,2-diphenyl1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma methods. Results: Results showed that Gram-negative bacteria and fungus were resistant to various plant extracts. Against all Gram-positive bacteria tested, C. coluteoides’ flower extract had the highest inhibition effects. Also, the most sensitive bacterium was Bacillus cereus, which had an 18-mm inhibition zone. Due to the solvent’s physical and chemical properties, different C. coluteoides extracts exhibited various antioxidant activities in the antioxidant activity assay. To some extent, methanol extract of leaves showed the highest DPPH radical scavenging activity at various concentrations that ranged from 5 to 160 mg.mL-1. The methanol extract of flower was observed to have the highest level of phenolics among all tested extracts. Conclusion: This study demonstrates that different extracts from various C. coluteoides parts are different in their properties, therefore, a proper solvent should be used to extract maximum amounts of antioxidant and antibacterial components from a typical plant material. Key words: Cleome coluteoides, antibacterial, antifungal, antioxidant

Özet

Amaç: Cleome coluteoides, Capparidaceae familyasına aittir ve uzun süredir geleneksel tıpta kullanılmaktadır. Çalışma C.coluteoides’in antioksidan, antibakteriyal ve antifungal aktivitesini ölçmeyi amaçlamıştır. Gereç ve Yöntemler: Bitkinin farklı kısımları etil asetat, metanol ve diklorometan gibi çeşitli çözücüler ile ekstre edildi. Antibakteriyel ve antifungal aktiviteler disk ve kuyu difüzyon yöntemleriyle, antioksidan aktivite ise 2,2-difenil-1-pikrilhidrazil (DPPH) ve ferrik indirgeme kabiliyeti ile belirlendi. Bulgular: Sonuçlar, Gram-negatif bakteri ve mantarların yukarıda bahsi geçen çeşitli bitki ekstrelerine karşı dayanıklı olduğunu gösterdi. C. coluteoides’in çiçek ekstresi, test edilen Gram-pozitif bakterilerin tümüne karşı en yüksek inhibitör etkiye sahipti. Ayrıca, Bacillus cereus, 18-mm inhibitör zonuyla en hassas bakteridir. Antioksidan aktivite analizinde, farklı C. coluteoides ekstreleri, solvanların fiziksel ve kimyasal özelliklerinden dolayı çeşitli antioksidan aktiviteler göstermişlerdir. Bir şekilde, yaprakların metanol ekstresi, 5-160 mg.mL-1 arasında değişen konsantrasyonlarda en yüksek DPPH serbest radikal süpürücü aktivite göstermiştir. Test edilen ekstreler arasında çiçeklerin metanol ekstresinin en yüksek fenolik içeriğe sahip olduğu bulundu. Sonuç: Bu çalışma, C. coluteoides’in çeşitli kısımlarının özelliklerinin farklı özelliklere sahip olduğunu göstermektedir, bu nedenle bir bitki materyalinin optimize edilmiş antioksidan ve antibakteriyel aktiviteleri için uygun bir çözücü bulmak hayati öneme sahiptir. Anahtar kelimeler: Cleome coluteoides, antibakteriyel, antifungal, antioksidan

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Cite This Article

MOGHADDAM, P. Z., MOHAMMADI, A., ALESHEIKH, P., FEYZI, P., HAGHBIN, A., MOLLAZADEH, S., SABETI, Z., NAKHLBAND, A., KASAIAN, J. (2022). Antibacterial, Antifungal, and Antioxidant Activity of Cleome coluteoides: An In Vitro Comparative Study Between Leaves, Stems, and Flowers. Turkish Journal of Pharmaceutical Sciences, Cilt: 18 - Sayı: 1, 10–16. https://ajindex.com/articles/613627

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