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AI Summary & Key Takeaways
AI Insight⚡ TL;DR(Too Long; Didn't Read)
More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.
📌 3 Key Takeaways
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Objective & Hypothesis:A method for isolating cardiac myocytes from the heart of guinea-pig is described.
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Key Findings & Data:This method consists of perfusion of the heart with a low Ca{2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme.
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Conclusion & Impact:More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
A method for isolating cardiac myocytes from the heart of guinea-pig is described. This method consists of perfusion of the heart with a low Ca{2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme. More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method.
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Cite This Article
MERAL, İ. (2022). An enzymatic method for isolating cardiac myocytes from heart of guinea-pig used for single-cell and patch-clamp studies. Turkish Journal of Medical Sciences, Cilt: 27 - Sayı: 6, 565–567. https://ajindex.com/articles/612707