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Journals / Turkish Journal of Medical Sciences / 2005 / Cilt: 35 - Sayı: 2

The effect of glutathione S-transferase M1 genotype on benzo[alpha]pyrene-induced sister chromatid exchanges and chromosomal aberrations in peripheral blood lymphocytes

Mehmet GÜVEN
İlhan ONARAN
Turgut ULUTİN
Gülgün S. GÜVEN
Seniha HACIHANEFİOĞLU
Pages
79–84
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

These findings suggest that SCEs and CAs induced in vitro by BaP are not influenced by the genotype GSTM1.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Sister chromatid exchange (SCE) and chromosomal aberration (CA) in peripheral lymphocytes have been widely used in assessing exposure to mutagens and carcinogens.
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Key Findings & Data:The possible influence of genetic polymorphism was also taken into account, by including donors representing positive or null glutathione S-transferase M1 (GSTM1) genotypes.
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Conclusion & Impact:These findings suggest that SCEs and CAs induced in vitro by BaP are not influenced by the genotype GSTM1.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Sister chromatid exchange (SCE) and chromosomal aberration (CA) in peripheral lymphocytes have been widely used in assessing exposure to mutagens and carcinogens. One of the extensively studied genotoxins is benzo[alpha]pyrene (BaP). The aim of the present study was to examine the ability of (BaP) to induce different individual cytogenetic response measured by SCE and CA frequency. The possible influence of genetic polymorphism was also taken into account, by including donors representing positive or null glutathione S-transferase M1 (GSTM1) genotypes. SCEs and CAs were analyzed from 72-h whole-blood lymphocyte cultures of 16 GSTM1 positive and 15 GSTM1 null donors after treatment with 5 µM BaP, which do not decrease cell viability. We found no influence of the GSTM1 genotype on SCE or CA frequency. The rates of chromatid and chromosome-type gaps and breaks induced in vitro by BaP were similar in all the groups. In the GSTM1 null genotype, however, chromatid-type breaks were seen more frequently than chromosome-type breaks after a 48 h treatment with BaP. These findings suggest that SCEs and CAs induced in vitro by BaP are not influenced by the genotype GSTM1.

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Cite This Article

GÜVEN, M., ONARAN, İ., ULUTİN, T., GÜVEN, G. S., HACIHANEFİOĞLU, S. (2022). The effect of glutathione S-transferase M1 genotype on benzo[alpha]pyrene-induced sister chromatid exchanges and chromosomal aberrations in peripheral blood lymphocytes. Turkish Journal of Medical Sciences, Cilt: 35 - Sayı: 2, 79–84. https://ajindex.com/articles/611995

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References (14)

  1. Gupta R, Earley C, Sharma S. Use of human peripheral blood lymphocytes to measure DNA binding capacity of chemical carcinogens. Proc Natl Acad Sci USA 85: 3513-3517, 1988
  2. Nadon L, Siemiatycki J, Dewar R et al. Cancer risk due to occupational exposure to polycyclic aromatic hydrocarbons. Am J Ind Med 28: 303-324, 1995
  3. Vineis P, Caporaso N. Tobacco and cancer: epidemiology and the laboratory. Environ Health Perspect 103: 156-160, 1995
  4. Pastorelli R, Guanci,M, Cerri A et al. Impact of inherited polymorphisms in glutathione S-transferase Ml, microsomal epoxide hydrolase, cytochrome P450 enzymes on DNA, and blood protein adducts of benzo(a)pyrene-diolepoxide. Cancer Epidemiol Biom Prevent 7: 703-709, 1998
  5. Gelboin HV. Benzo[oc]pyrene metabolism, activation, and carcinogenesis: role and regulation of mixed-function oxidases and related enzymes. Physiol Rev 60: 1107-1166, 1980
  6. Okano P, Miller HN, Robinson RC et al. Comparison of benzo[a]pyrene and (-)trans-7,8-dihydroxy-7,8-dihydrobenzo[cc] pyrene metabolism in human blood monocytes and lymphocytes. Cancer Res 39: 3184-3193, 1979
  7. Hayes JD, Pulford DJ. The glutathione S-transferase supergene family regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol 30: 445-600, 1995
  8. Board P, Coggan M, Johnston P et al. Genetic heterogeneity of the human glutathione transferases: a complex of gene families. Pharm Ther 48: 357-369, 1990
  9. Nielsen PS, De Pater N, Okkels H et al. Environmental air pollution and DNA adducts in Copenhagen bus drivers — effect of GSTM1 and NAT2 genotypes on adduct levels. Carcinogenesis 17: 1021-1027, 1996
  10. Wiencke JK, Kelsey KT, Lamela RA et al. Human glutathione S-transferase deficiency as a marker of susceptibility to epoxide-induced cytogenetic damage. Cancer Res 50: 1585-1590, 1990
  11. Harrison DJ, Cantlay AM, Rae F et al. Frequency of glutathione S-transferase Ml deletion in smokers with emphysema and lung cancer. Hum Exp Toxico 16: 356-360, 1997
  12. Zhong S, Willie AH, Barnes D et al. Relationship between the GSTM1 genetic polymorphism and susceptibility to bladder, breast and colon cancer. Carcinogenesis 14: 1821-1824, 1993
  13. Miller SA, Dykes DD, Polesky MF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215, 1988
  14. Tucker JD, Auletta A, Cimino MC et al. Sister-chromatid exchange: second report of the Gene-Tox Program. Mut