Investigations of ALS1 and HWP1 genes in clinical isolates of Candida albicans

Conclusion: It was concluded that various genes other than those evaluated could be present in slime formation of C. albicans, and the presence of the genes may not always be represented in the phenotype.

📌 3 Key Takeaways

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Objective & Hypothesis:To explore the presence of ALS1 and HWP1 genes by multiplex polymerase chain reaction (PCR) in Candida albicans strains.

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Key Findings & Data:Materials and methods: By using the multiplex PCR method, the presence of agglutinin-like sequence 1 (ALS1) and hyphal wall protein 1 (HWP1) genes were investigated in 206 C. albicans strains that were isolated from various clinical samples.

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Conclusion & Impact:Conclusion: It was concluded that various genes other than those evaluated could be present in slime formation of C. albicans, and the presence of the genes may not always be represented in the phenotype.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Key words: Candida albicans · ALS1 · HWP1

Abstract

To explore the presence of ALS1 and HWP1 genes by multiplex polymerase chain reaction (PCR) in Candida albicans strains. Materials and methods: By using the multiplex PCR method, the presence of agglutinin-like sequence 1 (ALS1) and hyphal wall protein 1 (HWP1) genes were investigated in 206 C. albicans strains that were isolated from various clinical samples. Phenotypic identification of slime formation by microplate and tube adherence tests was performed. Results: The presence of the ALS1 gene was detected in 53.9% of all strains, while the HWP1 gene was present in 5.3%. Slime formation was phenotypically detected in the 62.2% of the strains in which the ALS1 and/or the HWP1 gene was found, using the microplate and/or tube adherence test. The genes evaluated were found to be present in the 76.7% of strains in which slime formation was detected by phenotypic tests. There was a moderate correlation between the presence of the ALS1 gene and the microplate method, yet there was no correlation when using the tube adherence test. Conclusion: It was concluded that various genes other than those evaluated could be present in slime formation of C. albicans, and the presence of the genes may not always be represented in the phenotype.

Özet

To explore the presence of ALS1 and HWP1 genes by multiplex polymerase chain reaction (PCR) in Candida albicans strains. Materials and methods: By using the multiplex PCR method, the presence of agglutinin-like sequence 1 (ALS1) and hyphal wall protein 1 (HWP1) genes were investigated in 206 C. albicans strains that were isolated from various clinical samples. Phenotypic identification of slime formation by microplate and tube adherence tests was performed. Results: The presence of the ALS1 gene was detected in 53.9% of all strains, while the HWP1 gene was present in 5.3%. Slime formation was phenotypically detected in the 62.2% of the strains in which the ALS1 and/or the HWP1 gene was found, using the microplate and/or tube adherence test. The genes evaluated were found to be present in the 76.7% of strains in which slime formation was detected by phenotypic tests. There was a moderate correlation between the presence of the ALS1 gene and the microplate method, yet there was no correlation when using the tube adherence test. Conclusion: It was concluded that various genes other than those evaluated could be present in slime formation of C. albicans, and the presence of the genes may not always be represented in the phenotype.

Keywords

Key words: Candida albicans ALS1 HWP1 slime formation

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Cite This Article

ÖZER, B., DURAN, N., ATALAY, M. A., ÖNLEN, Y., KOÇ, A. N., EVİRGEN, Ö., İNCİ, M., DURMAZ, S., KÖKSALDI, V. M., KILINÇ, Ç. (2022). Investigations of ALS1 and HWP1 genes in clinical isolates of Candida albicans. Turkish Journal of Medical Sciences, Cilt: 43 - Sayı: 1, 125–130. https://ajindex.com/articles/611035

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