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Journals / Turkish Journal of Medical Sciences / 2020 / Cilt: 50 - Sayı: 8

Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.

Ayşe BARIŞ
Rabiye ALTINBAŞ
Sümeyye ŞEN
Recep ÖZTÜRK
Nuri KİRAZ
Pages
2024–2031
Publication Date
2022-11-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates.

📌 3 Key Takeaways

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Objective & Hypothesis:Background and aim: Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality.
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Key Findings & Data:The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB.
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Conclusion & Impact:Conclusions: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background and aim: Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality. Early diagnosis and identification of Candida spp. is important for the determination of antifungal agents that will be used for treatment. The aim of the present study was to provide a better regimen for Candida infections in the future. Materials and methods: The Sensititre YeastOne (SYO) method was compared with The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) testing method. Endpoints of minimal inhibitory concentrations (MICs) were determined for both methods. Results: By using both methods, MIC values of micafungin, caspofungin, voriconazole, and fluconazole were lower than amphotericin B. The values obtained with the SYO method were in high categorical agreement for ecinocandins and amphotericin B. The results of voriconazole and fluconazole were in low categorical agreement. The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB. Values obtained with SYO method for all antifungal agents were in high essential agreement with the data of the CLSI reference BMD method. The essential agreement between the SYO and the BMD results at 24 h was 94.0% for MFG and 99.0% for AMB. Conclusions: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates.

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Cite This Article

BARIŞ, A., ALTINBAŞ, R., ŞEN, S., ÖZTÜRK, R., KİRAZ, N. (2022). Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candida spp.. Turkish Journal of Medical Sciences, Cilt: 50 - Sayı: 8, 2024–2031. https://ajindex.com/articles/609763

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References (12)

  1. Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clinical Microbiology Reviews 2007
  2. 20 (1): 133-163. doi: 10.1128/CMR.00029-06 2. Tortorano AM, Kibbler C, Peman J, Bernhardt H, Klingspor L et al. Candidaemia in Europe: epidemiology and resistance. International Journal of Antimicrobial Agents 2006
  3. 27 (5): 359-366. doi: 10.1016/j.ijantimicag.2006.01.002 3. Hospenthal DR, Murray CK, Rinaldi MG. The role of antifungal susceptibility testing in the therapy of candidiasis. Diagnostic Microbiology and Infectious Disease 2004
  4. 48 (3): 153-160. doi: 10.1016/j.diagmicrobio.2003.10.003 4. Pema´n J, Zaragoza R. Current diagnostic approaches to invasive candidiasis in critical care settings. Mycoses 2009
  5. doi: 10.1111/j.1439-0507.2009.01732.x 5. Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts
  6. approved standard, 3rd edition, CLSI document M27-A3. Wayne, PA: Clinical and Laboratory Standards Institute
  7. Clinical and Laboratory Standards Institute. Reference method for broth dilution antifungal susceptibility testing of yeasts. 4th informational supplement, CLSI M27-S4. Wayne, PA: Clinical and Laboratory Standards Institute
  8. Pfaller MA, Messer SA, Hollis RJ, Espinel-Ingroff A, Ghannoum MA et al. Multisite reproducibility of MIC results by the Sensititre YeastOne colorimetric antifungal susceptibility panel. Diagnostic Microbiology and Infectious Disease 1998
  9. 31 (4): 543-547. doi: 10.1016/s0732-8893(98)00026-1 8. Hazen KC, Howell SA. Candida, Cryptococcus, and other yeasts of medical importance. In: Murray PR, Baron EJ, Jorgensen JH, Laudry ML, Pfaller MA, editors. Manual of clinical microbiology. 9th ed. Washington DC, ASM Press
  10. Larone DH. Medically important fungi, a guide to identification, 5th ed. Washington DC, USA: ASM Press
  11. Kiraz N, Dag I, Oz Y, Yamac M, Kiremitci A et al. Correlation between broth microdilution and disk diffusion methods for antifungal susceptibility testing of caspofungin, voriconazole, amphotericin B, itraconazole and fluconazole against Candida glabrata. Journal of Microbiological Methods 2010
  12. doi: 10.1016/j.mimet.2010.05.002 11. Pfaller MA, Diekema DJ. Progress in antifungal susceptibility testing of Candida spp. by use of Clinical and Laboratory Standards Institute broth microdilution methods, 2010 to 2012. Journal of Clinical Microbiolo