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Journals / Türk Biyokimya Dergisi / 2017 / Cilt: 42 - Sayı: 4

Hepatoprotective role of Solenostemma argel growing in Egypt on ethanol induced oxidative damage in rats

Mısırda büyüyen Solenostemma argel’in sıçanlarda etanole bağlı oksidatif hasara karşı karaciğer korumasındaki rolü

Mahgoub Mohamed AHMED
Pages
419–426
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

S. argel was given 1 h prior to EtOH by oral gavage for 28 days.Results: The results showed that, administration of EtOH caused a significant decrease (p < 0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The objective of the current study is to investigate the protective effect of Solenostemma argel (S. argel) treatment on ethanol (EtOH)-induced hepatotoxicity in rat liver.Methods: Forty adult male albino rats were divided into four groups as control, S. argel (100 mg/kg), EtOH (3 g/kg) and S. argel + EtOH groups.
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Key Findings & Data:S. argel was given 1 h prior to EtOH by oral gavage for 28 days.Results: The results showed that, administration of EtOH caused a significant decrease (p < 0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment.
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Conclusion & Impact:Co-administration of S. argel with EtOH significantly reversed the inflammation in rat liversConclusion: S. argel had a hepatoprotective role against EtOH-induce oxidative stress and inflammation in rat liver
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The objective of the current study is to investigate the protective effect of Solenostemma argel (S. argel) treatment on ethanol (EtOH)-induced hepatotoxicity in rat liver.Methods: Forty adult male albino rats were divided into four groups as control, S. argel (100 mg/kg), EtOH (3 g/kg) and S. argel + EtOH groups. S. argel was given 1 h prior to EtOH by oral gavage for 28 days.Results: The results showed that, administration of EtOH caused a significant decrease (p < 0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment. S. argel treatment significantly (p < 0.05) ameliorated the previous parameters. Protein carbonyl, reduced glutathione and LPO levels were significantly (p < 0.05) increased along with glutathione-S-transferase activity was decreased (as markers of oxidative stress) in EtOH treated rat liver. Previous oxidative stress was attenuated significantly by S. argel treatment. Moreover, in EtOH group, tumor necrosis factor-α (TNF-α) and nitric oxide (NO) contents and cytochrome P450 2E1 activity were significantly increased in liver tissues showing oxidative organ damage. Co-administration of S. argel with EtOH significantly reversed the inflammation in rat liversConclusion: S. argel had a hepatoprotective role against EtOH-induce oxidative stress and inflammation in rat liver

Özet

Amaç: Solenostemma argel (S. argel) tedavisinin, sıçanlarda etanole (EtOH) bağlı karaciğer toksisitesine karşı koruyucu etkisinin araştırılması. Metod: Kırk adet yetişkin erkek albino sıçan dört gruba ayrıldı: kontrol, S. argel (100 mg/kg), EtOH (3 g/kg) ve S. Argel + EtOH grupları. S. argel, EtOH uygulamasından bir saat önce 28 gün boyunca ağızdan beslenmiştir. Bulgular: Veriler EtOH uygulamasının serum toplam protein (TP) oranında ve albümin (Alb) oranında anlamlı bir azalmaya (p < 0.05) sebep olduğunu gösterdi, bunun yanısıra EtOH uygulaması sonucunda ALT ve AST ve lipid peroksidasyonunda (LPO) bir artış gözlendi. S. argel uygulaması bu parametrelerin anlamlı (p < 0.05) bir şekilde iyileşmesine sebep oldu. EtOH e maruz bırakılan sıçanların karaciğerlerinde (oksidatif stres markörü olarak) glutationS-transferaz (GST) aktivite azalmasının yanısıra, protein karbonil (PC), indirgenmiş glutation (GSH) ve LPO seviyeleri anlamlı bir şekilde artmıştır (p < 0.05). S. argel tedavisi gözlenen bu oksidatif stres anlamlı bir şekilde azaltmıştır. Hatta EtOH gurubu karaciğer dokusunda, oksidatif doku hasarını gösteren, tümör nekrosis faktör- ? (TNF-?) ve nitric okside (NO) seviyeleri ve cytokrom P450 2E1 (CYTP2E1) aktivitesi anlamlı bir şekilde artmıştır. S. argel ve EtOH'ün birlikte uygulanması, sıçan karaciğerinde inflamasyonun anlamlı bir şekilde geri çevrilmesine yol açmıştır.S. argel sıçan karaciğerinde EtOH'e bağlı oksidatif stres ve infalamasyon a karşı karaciğeri koruyucu bir role sahiptir.

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Cite This Article

AHMED, M. M. (2022). Hepatoprotective role of Solenostemma argel growing in Egypt on ethanol induced oxidative damage in rats. Türk Biyokimya Dergisi, Cilt: 42 - Sayı: 4, 419–426. https://ajindex.com/articles/609168

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