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Journals / Turkish Journal of Immunology / 2020 / Cilt: 8 - Sayı: 3

IL-10-592 A/C Gene Polymorphism and Cytokine Levels are Associated with Susceptibility to Drug Resistance in Tuberculosis

Sitokin Seviyelerinin ve IL-10-592 A/C Polimorfizminin Tüberkülozda İlaç Direnci ile İlişkisi

Farhad SEIF
Majid KHOSHMIRSAFA
Atefeh SADEGHI SHERMEH
Ali-Akbar DELBANDI
Payam TABARSI
Esmaeil MORTAZ
Mohammad Reza BOLOURI
Elham ALIPOUR FAYEZ
Mehdi SHEKARAB
Pages
103–112
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

The serum levels of IFN-γ were significantly higher in DS-TB patients than that of the other two groups (p

📌 3 Key Takeaways

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Objective & Hypothesis:Introduction: Tuberculosis (TB) and especially resistant forms of it have a substantial economic burden on the community health system for diagnosis and treatment each year.
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Key Findings & Data:Material and Methods: In this research SNPs in IFN-γ (+874 T/A) and IL-10 (-592 A/C) genes, and the effects of these SNPs on cytokine levels in a total of 87 tuberculosis patients and 100 healthy controls (HCs) were studied.
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Conclusion & Impact:The serum levels of IFN-γ were significantly higher in DS-TB patients than that of the other two groups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Introduction: Tuberculosis (TB) and especially resistant forms of it have a substantial economic burden on the community health system for diagnosis and treatment each year. Thus, investigation of this field is a priority for the world health organization (WHO). Cytokines play important roles in the relationship between the immune system and tuberculosis. Genetic variations especially single nucleotide polymorphisms (SNPs) impact cytokine levels and function against TB. Material and Methods: In this research SNPs in IFN-γ (+874 T/A) and IL-10 (-592 A/C) genes, and the effects of these SNPs on cytokine levels in a total of 87 tuberculosis patients and 100 healthy controls (HCs) were studied. TB patients divided into two groups: 1) 67 drug-sensitive (DS-TB) and 2) 20 drug-resistant (DR-TB) according to drug sensitivity test using polymerase chain reaction (PCR). For the genotyping of two SNPs, the PCR-based method was used and IFN-γ and IL-10 levels were measured by ELISA in pulmonary tuberculosis (PTB) and control group. Results: In -592A/C SNP, only two genotypes (AA, AC) were observed and both genotypes showed statistically significant differences between DR-TB and HCs (p=0.011). IL-10 serum levels in PTB patients were higher than HCs (p=0.02). The serum levels of IFN-γ were significantly higher in DS-TB patients than that of the other two groups (p

Özet

Giriş: Tüberküloz (TB) ve özellikle dirençli tiplerinin tanı ve tedavisi halk sağlığı sistemine önemli bir ekonomik yüke neden olmaktadır. Bu neden ile, bu konuda araştırma yapılması Dünya Sağlık Örgütü (DSÖ) tarafından önceliği olduğu bildirilen bir konudur. Sitokinleri bağışıklık sistemi ve tüberkülozda önemli rol oynamaktadır. Özellikle tek nükleotidde saptanan polimorfizmlerin (TNP) olduğu genetik farklılıklar tüberküloza karşı bağışıklık ve sitokin üretiminde etkilidir. Gereç ve Yöntemler: Bu çalışmada, 87 hasta ve 100 sağlıklı bireyde IFN-γ (+874 T/A) ve IL-10 (-592 A/C)’un genlerinin TNP’leri ve bu TNP’lerin oluşan sitokinlerin salınımına olan etkileri irdelendi. TB hastaları 2 ayrı gruba ayrıldı: Polimeraz zincir tepkimesi (PZT) ile ilaca hassas (İH) olduğu belirlenen 67 ve ilaca dirençli (İD) olduğu saptanan 20 tüberkülozlu hasta.TNP’ni saptamada PZT temelli bir metod, IFN-γ ve IL-10 düzeyleri için ise, ELISA yöntemi kullanıldı. Bulgular: Sağlıklı bireyler ve İD TB hastalar arasında -592A/C TNP açısından istatistiksel açıdan anlamlı farklılık saptandı (p=0.011). Akciğer tüberkülozu olan olgulardaki IL-10 düzeyleri sağlıklı bireylerdekine göre yüksek olarak saptandı (p=0.02). İH TB hastalarında serum IFN-γ düzeylerinin diğer gruplardakine göre daha yüksek olduğu saptandı (p

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Cite This Article

SEIF, F., KHOSHMIRSAFA, M., SHERMEH, A. S., DELBANDI, A., TABARSI, P., MORTAZ, E., BOLOURI, M. R., FAYEZ, E. A., SHEKARAB, M. (2022). IL-10-592 A/C Gene Polymorphism and Cytokine Levels are Associated with Susceptibility to Drug Resistance in Tuberculosis. Turkish Journal of Immunology, Cilt: 8 - Sayı: 3, 103–112. https://ajindex.com/articles/607192

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