SavedAdd your journal

Journals / Turkish Journal of Botany / 2019 / Cilt: 43 - Sayı: 3

loning of lipase cDNA from Olea europaea cv. Gemlik leaves and expression analysis in response to cold stress

Munise YURTSEVER
Şenay VURAL KORKUT
Pages
290–297
Publication Date
2022-11-04
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Multiple sequence alignment analysis of the putative protein sequence revealed that the enzyme containsconserved GXSXG pentapeptide motif, and the catalytic triad was predicted as Ser-182, His-379, and Asp-350 with bioinformatics tools.Real-time PCR was used to determine relative expression levels of lipase in leaves in response to cold stress.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The cDNA encoding lipase enzyme was cloned from Olea europaea var. europaea cv.
📊
Key Findings & Data:Gemlik leaves using the RACE method.The full nucleotide sequence was 1546 bp long and encoded a putative protein of 412 amino acid long protein.
💡
Conclusion & Impact:Multiple sequence alignment analysis of the putative protein sequence revealed that the enzyme containsconserved GXSXG pentapeptide motif, and the catalytic triad was predicted as Ser-182, His-379, and Asp-350 with bioinformatics tools.Real-time PCR was used to determine relative expression levels of lipase in leaves in response to cold stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The cDNA encoding lipase enzyme was cloned from Olea europaea var. europaea cv. Gemlik leaves using the RACE method.The full nucleotide sequence was 1546 bp long and encoded a putative protein of 412 amino acid long protein. The amino acid sequenceshowed identity to Triacylglycerol Lipase 1 sequences from different plants. A phylogenetic tree was constructed showing relationswith various plant lipases. Multiple sequence alignment analysis of the putative protein sequence revealed that the enzyme containsconserved GXSXG pentapeptide motif, and the catalytic triad was predicted as Ser-182, His-379, and Asp-350 with bioinformatics tools.Real-time PCR was used to determine relative expression levels of lipase in leaves in response to cold stress.

🔖

Cite This Article

YURTSEVER, M., KORKUT, Ş. V. (2022). loning of lipase cDNA from Olea europaea cv. Gemlik leaves and expression analysis in response to cold stress. Turkish Journal of Botany, Cilt: 43 - Sayı: 3, 290–297. https://ajindex.com/articles/600880

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (2)

  1. Abdelkafi S, Fouquet B, Barouh N, Durner S, Pina M, et al. (2009). In vitro comparisons between Carica papaya and pancreatic lipases during test meal lipolysis: Potential use of CPL in enzyme replacement therapy. Food Chemistry 115(2): 488- 494. doi: 10.1016/j.foodchem.2008.12.043 Ellinger D, Kubigsteltig II (2010). Involvement of DAD1-like lipases in response to salt and osmotic stress in Arabidopsis thaliana. Plant Signaling and Behavior 5(10): 1269-1271. doi: 10.4161/ psb.5.10.13012 Hills MJ, Mukherjee KD (1990). Triacylglycerol lipase from rape (Brassica napus L.) suitable for biotechnological purposes. Applied Biochemistry Biotechnology 26(1): 1-10. doi: 10.1007/ BF02798388 Hong JK, Choi HW, Hwang IS, Kim DS, Kim NH et al. (2008). Function of a novel GDSL-type pepper lipase gene, CaGLIP1, in disease susceptibility and abiotic stress tolerance. Planta 227(3): 539-558. doi: 10.1007/s00425-007-0637-5
  2. Inoue H, Nojima H, Okayama H (1990). High efficiency transformation of Escherichia coli with plasmids. Gene 96(1): 23-28. doi: 10.1016/0378-1119(90)90336-P Jakab G, Manrique A, Zimmerli L, Me J, Mauch-mani B (2007). Molecular characterization of a novel lipase-like pathogeninducible gene family of Arabidopsis. Plant Physiology 132: 2230-2239. doi: 10.1104/pp.103.025312 Ke T, Klibanov AM (1999). Markedly enhancing enzymatic enantioselectivity in organic solvents by forming substrate salts. Journal of the American Chemical Society 121(14): 3334- 3340. doi: 10.1021/ja984283v Lo M (2004). Characterization of an ultraviolet B-induced lipase in Arabidopsis. Plant Physiology 135(2): 947-958. doi 10.1104/ pp.103.036376 Mangos TJ, Jones KC, Foglia TA (1999). Lipase-catalyzed synthesis of structured low-calorie triacylglycerols. Journal of the American Oil Chemists’ Society 76(10): 1127-1132. doi: 10.1007/s11746- 999-0084-5 Olías JM (1993). Aroma of virgin olive oil: biogenesis of the “green” odor notes. Journal of Agricultural and Food Chemistry 41(12): 2368-2373. doi: 10.1021/jf00036a029 Padham AK, Hopkins MT, Wang TW, McNamara LM, Lo M et al. (2007). Characterization of a plastid triacylglycerol lipase from Arabidopsis. Plant Physiology 143(3): 1372-1384. doi: 10.1104/ pp.106.090811 Panzanaro S, Nutricati E, Miceli A, De Bellis L (2010). Biochemical characterization of a lipase from olive fruit (Olea europaea L.). Plant Physiology and Biochemistry 48(9): 741-745. doi: 10.1016/j.plaphy.2010.05.004 Pinyaphong P, Phutrakul S (2009). Synthesis of cocoa butter equivalen