SavedAdd your journal

Journals / Turkish Journal of Biology / 2017 / Cilt: 41 - Sayı: 5

Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro: a study emphasizing signaling molecule expression and transcriptional changes

Qiong WU
Chang-xin JIN
Xue-yong LI
Yue-jun LI
Hui CHEN
Pages
785–795
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments.
📊
Key Findings & Data:This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs.
💡
Conclusion & Impact:In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migrationrelated regulators in MCF-7 cells were significantly enhanced in both protein (Smad, Slug, Snail1/2, Twist1, N-cadherin, vimentin) and mRNA (SMAD, SLUG, SNAIL1/2, TWIST1, N-cadherin, and vimentin) levels. The expression levels of oncogenes (RAS and HER-2) and an antioncogene gene (P53) in ADSCs were upregulated and downregulated, respectively. Interestingly, variation tendencies of the molecules were more conspicuous in inflammatory circumstances than without. In conclusion, interaction of MCF-7 cells with ADSCs increases the levels of a series of metastasis-related functional proteins in MCF-7 cells and enhances the expression of oncogenes in ADSCs.

🔖

Cite This Article

WU, Q., JIN, C., LI, X., LI, Y., CHEN, H. (2022). Interaction of adipose-derived mesenchymal stem cells with MCF-7 cells in vitro: a study emphasizing signaling molecule expression and transcriptional changes. Turkish Journal of Biology, Cilt: 41 - Sayı: 5, 785–795. https://ajindex.com/articles/600337

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (9)

  1. Abd Elmageed ZY, Yang Y, Thomas R, Ranjan M, Mondal D, Moroz K, Fang Z, Rezk BM, Moparty K, Sikka SC et al. (2014). Neoplastic reprogramming of patient-derived adipose stem cells by prostate cancer cell-associated exosomes. Stem Cells 32: 983-997.
  2. Bolontrade MF, Sganga L, Piaggio E, Viale DL, Sorrentino MA, Robinson A, Sevlever G, García MG, Mazzolini G, Podhajcer OL (2012). A specific subpopulation of mesenchymal stromal cell carriers overrides melanoma resistance to an oncolytic adenovirus. Stem Cells Dev 21: 2689-2702.
  3. Chen X, McClurg A, Zhou GQ, McCaigue M, Armstrong MA, Li G (2007). Chondrogenic differentiation alters the immunosuppressive property of bone marrow- derived mesenchymal stem cells, and the effect is partially due to the upregulated expression of B7 molecules. Stem Cells 25: 364- 370. Cho JA, Park H, Lim EH, Lee KW (2012). Exosomes from breast cancer cells can convert adipose tissue-derived mesenchymal stem cells into myofibroblast-like cells. Int J Oncol 40: 130-138.
  4. Durai P, Batool M, Choi S (2015). Structure and effects of cyanobacterial lipopolysaccharides. Mar Drugs 13: 4217-4230.
  5. Hood JL, Pan H, Lanza GM, Wickline SA; Consortium for Translational Research in Advanced Imaging and Nanomedicine (C-TRAIN) (2009). Paracrine induction of endothelium by tumor exosomes. Lab Invest 89: 1317-1328.
  6. Javelaud D, Alexaki VI, Dennler S, Mohammad KS, Guise TA, Mauviel A (2011). TGF-β/SMAD/GLI2 signaling axis in cancer progression and metastasis. Cancer Res 71: 5606-5510.
  7. Jiang HL, Sun HF, Gao SP, Li LD, Hu X, Wu J, Jin W (2015). Loss of RAB1B promotes triple-negative breast cancer metastasis by activating TGF-β/SMAD signaling. Oncotarget 6: 16352- 16365. Jotzu C, Alt E, Welte G, Li J, Hennessy BT, Devarajan E, Krishnappa S, Pinilla S, Droll L, Song YH (2011). Adipose tissue derived stem cells differentiate into carcinoma- associated fibroblastlike cells under the influence of tumor derived factors. Cell Oncol (Dordr) 34: 55-67.
  8. Kallergi G, Papadaki MA, Politaki E, Mavroudis D, Georgoulias V, Agelaki S (2011). Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patients. Breast Cancer Res 13: R59. Khurshid R, Saleem M, Gul-e-Raana, Akhthar MS (2014). Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease. Acta Biochim Pol 61: 699-703.
  9. Kilroy GE, Foster SJ, Wu X, Ruiz J, Sherwood S, Heifetz A, Ludlow JW, Stricker DM, Potiny S, Green P et al. (2007). Cytokine prof