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Journals / Turgut Özal Tıp Merkezi Dergisi / 2018 / Cilt: 25 - Sayı: 1

The comparison of the mrna expressions of Tgf?1, bfgf, ıgf-1, ngf and matrix metalloprotease ııı genes in cervical and lumbar disc tissues

Cem OZİC
Seyho Cem YUCETAS
Atilla YİLMAZ
Elif GULEC
Pages
92–95
Publication Date
2022-10-18
DOI
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Also the TGF?1, bFGF, IGF-1, NGF, and Matrix metalloprotease III genes showed a decrease in gene expression levels when they were analyzed by Real-time polymerase chain reaction.Conclusion: These data showed that; decrease of the TGF?1, bFGF, IGF-1, NGF and MMP III genes in the degenerated lumbar disc tissues, may related with the possibility of molecular background of the disease pathogenesis.

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Objective & Hypothesis:aim: To evaluate and compare the mRNA expression of antioxidant genes between the cervical and lumbar degenerated disc tissues.Material and Methods: Obtained degenerated Nucleus Pulposus (DNP) materials were divided into two groups, which were cervical DNP (Group I) (n=20) and lumbar DNP (Group II) (n=20).
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Key Findings & Data:There are 6 men and 14 women in group I with a mean age of 43.8 years and 13 men and 7 women in group II with the mean age of 40.3 years (28 to 54).
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Conclusion & Impact:Also the TGF?1, bFGF, IGF-1, NGF, and Matrix metalloprotease III genes showed a decrease in gene expression levels when they were analyzed by Real-time polymerase chain reaction.Conclusion: These data showed that; decrease of the TGF?1, bFGF, IGF-1, NGF and MMP III genes in the degenerated lumbar disc tissues, may related with the possibility of molecular background of the disease pathogenesis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

aim: To evaluate and compare the mRNA expression of antioxidant genes between the cervical and lumbar degenerated disc tissues.Material and Methods: Obtained degenerated Nucleus Pulposus (DNP) materials were divided into two groups, which were cervical DNP (Group I) (n=20) and lumbar DNP (Group II) (n=20). There are 6 men and 14 women in group I with a mean age of 43.8 years and 13 men and 7 women in group II with the mean age of 40.3 years (28 to 54). All cervical DNP materials were obtained between C5 and C6 and all lumbar DNP materials were obtained between L4 and L5 levels. The TGF?1, bFGF, IGF-1, NGF and Matrix metalloprotease III (MMP III) gene expressions of DNP materials were determined by polymerase chain reaction, Real-time polymerase chain reaction and western blotting techniques.Results: When the results of the two groups were compared by polymerase chain reaction, the expressions of TGF?1, bFGF, IGF-1, NGF and MMP III was found lower in lumbar DNP group. Also the TGF?1, bFGF, IGF-1, NGF, and Matrix metalloprotease III genes showed a decrease in gene expression levels when they were analyzed by Real-time polymerase chain reaction.Conclusion: These data showed that; decrease of the TGF?1, bFGF, IGF-1, NGF and MMP III genes in the degenerated lumbar disc tissues, may related with the possibility of molecular background of the disease pathogenesis.

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Cite This Article

OZİC, C., YUCETAS, S. C., YİLMAZ, A., GULEC, E. (2022). The comparison of the mrna expressions of Tgf?1, bfgf, ıgf-1, ngf and matrix metalloprotease ııı genes in cervical and lumbar disc tissues. Turgut Özal Tıp Merkezi Dergisi, Cilt: 25 - Sayı: 1, 92–95. https://ajindex.com/articles/599006

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References (13)

  1. Soini J, Antti-Poika I, Tallroth K, Konttinen YT, Honkanen V, Santavirta S. Disc degeneration and angular movement of the lumbar spine: comparative study using plain and flexion-extension radiography and discography. J Spinal Disord 1991;4(2):183-7
  2. Humzah MD, Soames RW. Human intervertebral disc: structure and function. Anat Rec 1988;220(4):337-56
  3. Mwale F, Iatridis JC, Antoniou J. Quantitative MRI as a diagnostic tool of intervertebral disc matrix composition and integrity. Eur Spine J 2008;17 Suppl 4:432-40
  4. Freemont TJ, LeMaitre C, Watkins A, Hoyland JA. Degeneration of intervertebral discs: current understanding of cellular and molecular events, and implications for novel therapies. Expert Rev Mol Med 2001;2001:1-10
  5. Le Maitre CL, Freemont AJ, Hoyland JA. Localization of degradative enzymes and their inhibitors in the degenerate human intervertebral disc. J Pathol 2004;204(1):47-54
  6. Teichtahl AJ, Urquhart DM, Wang Y, Wluka AE, Heritier S, Cicuttini FM. Response to: 'A dose-response relationship between severity of disc degeneration and intervertebral disc height in the lumbosacral spine'-authors' reply. Arthritis Res Ther 2016;18:45
  7. Yilmaz A, Senturk S, Sasani M, Oktenoglu T, Yaman O, Yildirim H, et al. Disc Rehydration after Dynamic Stabilization: A Report of 59 Cases. Asian Spine J 2017;11(3):348-55
  8. Suthar P, Patel R, Mehta C, Patel N. MRI evaluation of lumbar disc degenerative disease. J Clin Diagn Res 2015;9(4):TC04-09
  9. Saleem S, Aslam HM, Rehmani MA, Raees A, Alvi AA, Ashraf J. Lumbar disc degenerative disease: disc degeneration symptoms and magnetic resonance image findings. Asian Spine J 2013;7(4):322-34
  10. Su S, DiBattista JA, Sun Y, Li WQ, Zafarullah M. Up-regulation of tissue inhibitor of metalloproteinases-3 gene expression by TGF-beta in articular chondrocytes is mediated by serine/threonine and tyrosine kinases. J Cell Biochem 1998;70(4):517-27
  11. Thompson JP, Oegema TR Jr, Bradford DS. Stimulation of mature canine intervertebral disc by growth factors. Spine (Phila Pa 1976) 1991;16(3):253-60
  12. Li HR, Cui Q, Dong ZY, Zhang JH, Li HQ, Zhao L. Downregulation of miR-27b is ınvolved in loss of type II collagen by directly targeting matrix metalloproteinase 13 (mmp13) in human ıntervertebral disc degeneration. Spine (Phila Pa 1976) 2016;41(3):E116-23
  13. Sainoh T, Orita S, Miyagi M, Sakuma Y, Yamauchi K, Suzuki M, et al. Interleukin-6 and interleukin-6 receptor expression, localization, and involvement in p