Journals / Marmara Fen Bilimleri Dergisi / 2018 / Cilt: 30 - Sayı: 3

Transferability of Barley Retrotransposons (Sukkula and Nikita) to Investigate Genetic Structure of Pimpinella anisum L.

Arpa Retrotranspozonlarının (Sukkula ve Nikita) Pimpinella Anisum L.’nin Genetik Yapısını İncelemeye Aktarılabilirliği

Pages
299–304
Publication Date
2022-10-19
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results are expected to contribute a new perspective for genome architect of medically important plants in addition to aniseed.

📌 3 Key Takeaways

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Objective & Hypothesis:Transferability of barley retrotransposons (Nikita and Sukkula) were examined in Pimpinella anisum L. seeds by using a retrotransposon-based molecular marker: IRAP (inter-retrotransposon amplified polymorphism).
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Key Findings & Data:Furthermore, transposons’ sequences identified in medically important plants were obtained form NCBI, and multiple alignment analyses were performed to investigate the evolutionary relationships.
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Conclusion & Impact:Our results are expected to contribute a new perspective for genome architect of medically important plants in addition to aniseed.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Transferability of barley retrotransposons (Nikita and Sukkula) were examined in Pimpinella anisum L. seeds by using a retrotransposon-based molecular marker: IRAP (inter-retrotransposon amplified polymorphism). Furthermore, transposons’ sequences identified in medically important plants were obtained form NCBI, and multiple alignment analyses were performed to investigate the evolutionary relationships. These two retrotransposons were identified in Pimpinella anisum L., showing homomorphic band profiles. In addition, limited similar sequences were detected as a result of clustal analyses. Till date, no study about retrotransposons evaluation using IRAP as molecular marker has been published in aniseed. Our results are expected to contribute a new perspective for genome architect of medically important plants in addition to aniseed.

Özet

Arpa retrotranspozonlarının (Nikita ve Sukkula) aktarılabilirliği, retrotranspozon temelli bir moleküler markır olan IRAP (retrotranspozon-arası çoğaltılmış polimorfizm) yöntemi kullanılarak Pimpinella anisum L. tohumlarında incelendi. Ayrıca, tıbbi olarak önemli bitkilerde tanımlanan transpozonların dizileri, NCBI’dan elde edildi ve evrimsel ilişkileri araştırmak için çoklu hizalama analizleri yapıldı. Pimpinella anisum L. bitkisinde tanımlanan bu iki retrotranspozon homomorfik bant profili gösterdi. Bununla birlikte, çoklu hizalama analizleri sonucunda sınırlı sayıda benzer diziler tespit edildi. Bugüne kadar, anasonda IRAP gibi bir moleküler markır kullanılarak retrotranspozon değerlendirmesi yapan bir çalışma yayınlanmamıştır. Sonuçlarımızın, anason ile birlikte tıbbi açıdan önemli bitkilerin genom yapısının anlaşılması için yeni bir bakış açısına katkıda bulunması bekleniyor

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Cite This Article

MARAKLI, S. (2022). Transferability of Barley Retrotransposons (Sukkula and Nikita) to Investigate Genetic Structure of Pimpinella anisum L.. Marmara Fen Bilimleri Dergisi, Cilt: 30 - Sayı: 3, 299–304. https://ajindex.com/articles/578365

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