SavedAdd your journal

Journals / Kulak Burun Boğaz Uygulamaları / 2021 / Cilt: 9 - Sayı: 1

Histological examination of structural changes in the cochlear canal

Koklear kanaldaki yapısal değişikliklerin histolojik incelenmesi

Esra BALCIOĞLU
Betül YALÇIN
Pınar BİLGİCİ
Arzu Hanım YAY
Demet BOLAT
Ayşe CEYHAN
Mehmet Akif SOMDAŞ
Pages
12–17
Publication Date
2022-10-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

To determine the structural changes in the organ of Corti, basilar, tectorial, and Reissner membrane lengths, and heights of inner and outer hair cells were measured from 50 different areas using the Image J software and the data obtained were statistically evaluated.Results: It was determined that from the basal to the apex of the cochlea; the thickness of the basement membrane and the length of the Reissner's membrane decreased, while the length of the inner and outer hair cells and the length of the basement membraneincreased.Theseincreasesanddecreaseswerestatistically significant (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: Inthisstudy,weaimedtodeterminethemorphological changes in the organ of Corti in the basal, medial, and apical regions of the cochlear canal, which exhibit a complex structure.Materials and Methods: A total of eight male Wistar albino rats (35 days, 200 to 250 g) were decapitated under anesthesia.
📊
Key Findings & Data:The organ of Corti was quickly removed and fixed in 10% formaldehydesolutionforhistologicalexamination.Thetissues were, then, taken to decalcification solution.
💡
Conclusion & Impact:To determine the structural changes in the organ of Corti, basilar, tectorial, and Reissner membrane lengths, and heights of inner and outer hair cells were measured from 50 different areas using the Image J software and the data obtained were statistically evaluated.Results: It was determined that from the basal to the apex of the cochlea; the thickness of the basement membrane and the length of the Reissner's membrane decreased, while the length of the inner and outer hair cells and the length of the basement membraneincreased.Theseincreasesanddecreaseswerestatistically significant (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Inthisstudy,weaimedtodeterminethemorphological changes in the organ of Corti in the basal, medial, and apical regions of the cochlear canal, which exhibit a complex structure.Materials and Methods: A total of eight male Wistar albino rats (35 days, 200 to 250 g) were decapitated under anesthesia. The organ of Corti was quickly removed and fixed in 10% formaldehydesolutionforhistologicalexamination.Thetissues were, then, taken to decalcification solution. Following dehydration and clearing, they were embedded in paraffin. Sections were stained with hematoxylin & eosin and Masson's trichrome,andphotographswerecapturedusingalightmicroscope. To determine the structural changes in the organ of Corti, basilar, tectorial, and Reissner membrane lengths, and heights of inner and outer hair cells were measured from 50 different areas using the Image J software and the data obtained were statistically evaluated.Results: It was determined that from the basal to the apex of the cochlea; the thickness of the basement membrane and the length of the Reissner's membrane decreased, while the length of the inner and outer hair cells and the length of the basement membraneincreased.Theseincreasesanddecreaseswerestatistically significant (p

Özet

Amaç: Bu çalışmada kompleks bir yapı sergileyen koklear kanalın bazal, medial ve apikal bölgelerinde korti organındaki morfolojik değişiklikler belirlendi. Gereç ve Yöntemler: Toplam sekiz Wistar Albino cinsi erkeksıçan(35günlük,200-250g)anestezialtındadekapite edildi. Korti organı hızla çıkarıldı ve histolojik inceleme için %10 formaldehit solüsyonu içinde tespit edildi. Sonrasında dokular dekalsifikasyon solüsyonuna alındı. Dehidrasyon ve temizlemenin ardından parafine gömüldü. Kesitler hematoksilen- eozin ve Masson trikom ile boyandı ve ışık mikroskobu ile fotoğraf ları çekildi. Korti organındaki yapısal değişiklikleri belirlemek için baziler, tektorial ve Reissner membran uzunlukları ile iç ve dış saçlı hücrelerin boyları Image J yazılımı kullanılarak 50 farklı alanda ölçüldü ve elde edilen veriler istatistiksel olarak değerlendirildi. Bulgular: Kokleanın bazalından apeksine doğru gittikçe; bazal membran kalınlığı ve Reissner's membran uzunluğunu azalırken, iç ve dış saçlı hücrelerin uzunlukları ile bazal membran uzunluğunun arttığı belirlendi. Bu artış ve azalışlar istatistiksel olarak anlamlılık seviyesindeydi (p

🔖

Cite This Article

BALCIOĞLU, E., YALÇIN, B., BİLGİCİ, P., YAY, A. H., BOLAT, D., CEYHAN, A., SOMDAŞ, M. A. (2022). Histological examination of structural changes in the cochlear canal. Kulak Burun Boğaz Uygulamaları, Cilt: 9 - Sayı: 1, 12–17. https://ajindex.com/articles/576502

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (19)

  1. Akyıldız AN. Kulak Hastalıkları ve Mikrocerrahisi
  2. Cilt. Ankara: Bilimsel Tıp Yayınevi; 1998
  3. Karasalihoğlu AR. Kulak Burun Boğaz Hastalıkları ve Baş-Boyun Cerrahisi. Ankara: Güneş Kitabevi; 2003. s. 3-14
  4. Donaldson JA, Duckert LG. Anatomy of theears. In: Paparella MM, Shumrick DA, Gluckman JL, Meyerhoff WL, editors. Otolaryngology Head and Neck Vol. 1, 3rd ed. Philadelphia: WB Saunders Co; 1991. p. 23-58
  5. Chen WC, Jackson A, Budnick AS, Pfister DG, Kraus DH, Hunt MA, et al. Sensorineural hearing loss in combined modality treatment of nasopharyngeal carcinoma. Cancer 2006;106:820-9
  6. Kopelman J, Budnick AS, Sessions RB, Kramer MB, Wong GY. Ototoxicity of high-dose cisplatin by bolus administration in patients with advanced cancers and normal hearing. Laryngoscope 1988;98:858-64
  7. Santi PA, Mancini P. Kohlear anatomi ve santral işitme yolları. In: Koç C, editör. Cummings Otolaringoloji Baş ve Boyun Cerrahisi
  8. Cilt. Ankara: Güneş Tıp Kitabevleri; 2 0 0 7. s. 3373 -9 7
  9. Arıncı K, Elhan A. Kulak, işitme ve denge organları anatomisi. Anatomi. Ankara: Güneş Kitapevi; 1997. s. 466-90
  10. Raphael Y, Altschuler RA. Structure and innervation of the cochlea. Brain Res Bull 2003;60:397-422
  11. Poyrazoğlu E, Güngör A, Uğur G, Okar İ, Candan H. Ototopikal siprof loksasinin koklear etkilerinin taramali elektron mikroskopisi ile gösterilmesi. K.B.B. ve Baş Boyun Cerrahisi Dergisi 1997;5:116-21
  12. Dallos P. Overwiev; Cochlear Neurobiology. In; Dallos P, Popper AN, Fay RR, editors. The Cochlea. 1st ed. NewYork: Springer; 1996. p. 1-44
  13. Santi PA, Tsuprun VL. Cochlear Micro anatomy and Ultrastructure. In: AF Jahn, JS-Sacchi, editors. Physiology of the Ear. 2nd ed. San Diego; Singular & Thomson Learning; 2001. p. 257-81
  14. Slepeckly NB. The cochlear structure. In; Dallos P, Popper AN, Fay RR, editors. The Cochlea. 1st ed. NewYork: Springer; 1996; p. 44-130
  15. Donaldson JA, Duckert LG. Anatomy of the ears. In: Paparella MM, Shumrick DA, Gluckman JL, Meyerhoff WL, editors. Otolaryngology. Vol. 1. 3rd ed. Philadelphia: WB Saunders Co; 1991. p. 23-58
  16. John HM, Waren YA. Anatomy and phsiology of hearing. In: Bailey BJ, editor. Otolaryngol Head Neck Surgery Vol
  17. Philedelphia: JB Lippincott Co; 1993. p. 1441-61
  18. Harrison RV, Mount RJ. The Sensory Epithelium of The Normal and Pathological Cochlea. In: Jahn AF, S-Sacchi J, editors. Physiology of the Ear. 2nd ed. San Diego; Singular & Thomson Learning; 2001. p. 285-99
  19. Wysocki J. Topogr