The Effect of Ozone Treatment on Thermal Burn Wound Healing; an Experimental Study

But glutathionereductase enzyme activities were lover in the ozone treated group and hydroxyprolineconcentration decreased in ozone group compared to burn+normal saline group on 7and 21 days.Conclusions: Ozone has a detractive effect in the development of inflamation,fibrosis, and granulation via decreasing tissue damage and increasing the antioxidantenzyme activity on burn wound healing.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Ozone has been advised as a metabolic excitative in cell, immunomodulatory agent and antioxidant enzyme actuator.

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Key Findings & Data:The present study was performedto designate the profit of ozone therapy on the burn wound healing in the rats.Methods: 40 adult Wistar type rats divided into 4 groups: a control group (burn notcreated and topical and systemic agents not used), a burn group (burn created but onlysaline given), a burn+silver sulfadiazine group (silver sulfadiazine was applied for 21days), a burn+ozone/oxygen mixture group (ozone/oxygen mixture was applied for 21days).After anesthetizing, second-degree burn (2 cm2areas) was done on the dorsal ofthe animals byaluminum plate in boiling hot water (100 ˚C for 15 seconds) and othergroups except control group were treated topically, based on the time scheduled.Tissue samples were harvested on day 7st and 21st after burn injury.

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Conclusion & Impact:But glutathionereductase enzyme activities were lover in the ozone treated group and hydroxyprolineconcentration decreased in ozone group compared to burn+normal saline group on 7and 21 days.Conclusions: Ozone has a detractive effect in the development of inflamation,fibrosis, and granulation via decreasing tissue damage and increasing the antioxidantenzyme activity on burn wound healing.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Objective: Ozone has been advised as a metabolic excitative in cell, immunomodulatory agent and antioxidant enzyme actuator. The present study was performedto designate the profit of ozone therapy on the burn wound healing in the rats.Methods: 40 adult Wistar type rats divided into 4 groups: a control group (burn notcreated and topical and systemic agents not used), a burn group (burn created but onlysaline given), a burn+silver sulfadiazine group (silver sulfadiazine was applied for 21days), a burn+ozone/oxygen mixture group (ozone/oxygen mixture was applied for 21days).After anesthetizing, second-degree burn (2 cm2areas) was done on the dorsal ofthe animals byaluminum plate in boiling hot water (100 ˚C for 15 seconds) and othergroups except control group were treated topically, based on the time scheduled.Tissue samples were harvested on day 7st and 21st after burn injury. Biochemical andhistological analyzes were performed in tissue and blood samples. The results wereassessed with appropriate statistical tests and given as Means±SD.Results: The histopathologic damage level was significantly different in all groups.Fibrosis and inflamation levels decreased in ozone treatment and silver sulphadiazinegroups compared to burn+normal saline group on the 21 days according to 7 days. Inthe ozone treatment group, Glucose 6-phosphate dehydrogenase activities weresignificantly higher than the silver sulfadiazine treated group. But glutathionereductase enzyme activities were lover in the ozone treated group and hydroxyprolineconcentration decreased in ozone group compared to burn+normal saline group on 7and 21 days.Conclusions: Ozone has a detractive effect in the development of inflamation,fibrosis, and granulation via decreasing tissue damage and increasing the antioxidantenzyme activity on burn wound healing.

Özet

Amaç: Ozon, hücre içinde, immüno-modülatör unsur ve antioksidan enzim işleticisinde metabolik bir uyarıcı olarak önerilmiştir. Bu çalışma, sıçanlarda yanık yarası iyileşmesinde ozon tedavisinin yararını belirlemek için yapılmıştır. Gereç ve Yöntem: Bu çalışma için 40 adet Wistar tipi sıçan 4 gruba ayrıldı: Bir kontrol grubu (yanık oluşturulmadı, bölgesel ve sistemik unsurlar kullanılmadı), bir yanık grubu (yanık oluşturuldu, ancak sadece salin verildi), bir yanık + gümüş sülfadiazin grubu (21 gün boyunca gümüş sülfadiazin uygulandı), bir yanık + ozon / oksijen karışımı grubu (21 gün boyunca ozon / oksijen karışımı uygulandı) oluşturuldu. Anestezi uygulandıktan sonra, kaynar sıcak suda (15 saniye boyunca 100 ˚C) alüminyum plaka ile hayvanların sırtında ikinci derece yanık (2 cm2 alan) oluşturuldu ve kontrol grubu dışındaki diğer gruplar planlanan sürede bölgesel olarak tedavi edildi. Doku örnekleri yanık hasarından sonraki 7. ve 21. günlerde elde edildi. Doku ve kan örneklerinde biyokimyasal ve histolojik analizler yapıldı. Sonuçlar uygun istatistiksel testlerle değerlendirildi ve ± SD ortalamalar olarak verildi. Bulgular: Histopatolojik hasar düzeyi tüm gruplarda anlamlı olarak farklıydı. Ozon tedavisi ve gümüş sülfadiazin gruplarında fibroz ve inflamasyon seviyeleri, 7 güne göre 21 günde yanık + normal tuzlu su grubuna kıyasla azaldı. Ozon tedavi grubunda, Glikoz 6-fosfat dehidrogenaz aktiviteleri gümüş sülfadiazin ile tedavi edilen gruptan önemli ölçüde yüksekti. Ancak glutatyon redüktaz enzim aktiviteleri, ozon ile tedavi edilen grupta daha düşüktü ve hidroksiprolin konsantrasyonu, 7 ve 21 günde yanık + normal tuzlu su grubuna kıyasla ozon grubunda azaldı. Sonuç: Ozonun, doku hasarını azaltarak ve yanık yara iyileşmesinde antioksidan enzim aktivitesini artırarak iltihaplanma, fibrozis ve granülasyon gelişiminde bozucu bir etkisi bulunmaktadır.

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Cite This Article

DEMİRYILMAZ, İ., YILMAZ, İ., GÜRSUL, C., ÇOBAN, T. A., PEKER, K., ÇANKAYA, M., SAYAR, I., ISIK, A. (2022). The Effect of Ozone Treatment on Thermal Burn Wound Healing; an Experimental Study. KONURALP TIP DERGİSİ, Cilt: 12 - Sayı: 3, 511–518. https://ajindex.com/articles/575535

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References (13)

Jeschke MG, Chinkes DL, Finnerty CC, Kulp G, Suman OE, Norbury WB, et al. The pathophysıologıc response to severe burn injury. Ann Surg. 2008;248(3):387–401
Arturson G. Pathophysiology of the burn wound and pharmacological treatment. The Rudi Hermans Lecture. Burns. 1996;22(4):255-74
Gupta A, Keshri GK, Yadav A, Gola S, Chauhan S, Salhan AK, et al. Superpulsed (Ga-As, 904 nm) lowlevel laser therapy (LLLT) attenuates inflammatory response and enhances healing of burn wounds. Journal of Biophotonics. 2015;8(6):489-501
Edwards LC, Dunphy JE. Wound healing I. Injury and normal repair. N EngI J Med. 1958;259(5):224-33 5. Kutlubay Z, Engin B, Serdaroğlu S, Tüzün Y. Dermatolojide ozon tedavisi. Dermatoz. 2010;1(4):209-16
Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res. 2006;37(4):425- 35
Beutler E. Red cell metabolism: 12. London: Academic Press; 1971
Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 1996;29(3):225-9
Nalbandian RM, Henry RL, Balko KW, Adams DV, Neuman NR. Pluronic F-127 gel preparation as an artificial skin in the treatment of 3rd-degree burns in pigs. J Biomed Mater Res. 1987;21(9):1135-48. 10.Sheridan RL, Tompkins RG. Skin substitutes in burns. Burns. 1999;25(2):97-103
O’Connor NE, Mulliken JB, Banks-Schlegel S, Kehinde O, Green H. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet. 1981;1(8211):75–8. 12.Pearson AS, Wolford RW. Management of skin trauma. Primary Care. 2000;27(2):475-92
Brown GL, Curtsinger LJ, White M, Mitchell RO, Pietsch J, Nordquist R, et al. Acceleration of tensile strength of incisions treated with EGF and TGF-Beta. Ann Surg. 1988;208(6):788-94. 14.Fu XB, Shen ZY, Chen YL, Xie JH, Guo ZR, Zhang ML, et al. Randomised placebo-controlled trial of use of topical recombinant bovine basic fibroblast growth factor for second-degree burns. Lancet. 1998;352(9141):1661-4
Ueno H, Yamada H, Tanaka I, Kaba N, Matsuura M, Okumura M, et al. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials. 1999;20(15):1407-14
Mehlman MA, Borek C. Toxicity and biochemical-mechanisms of ozone. Environ Res. 1987;42(1):36-53. 17.Silva RA, Garotti JER, Silva RSB, Navarini A, Pacheco AM. Analysis of the bactericidal effect of ozone pneumoperitoneum. Acta Cir Bras. 2009;24(2):124-7
Bocci V. Ozone as a