Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2019 / Cilt: 25 - Sayı: 3

Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced with Diffrent Curing Processes

Farklı Kürleme İşlemleri İle Üretilen Pastırmada Laktik Asit Bakterilerinin Genotipik İdentifikasyonu

Pages
299–303
Publication Date
2022-10-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effctive than the curing temperature.

📌 3 Key Takeaways

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Objective & Hypothesis:The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two diffrent curing temperatures (4°C or 10°C) and two diffrent curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identifiation.
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Key Findings & Data:According to the identifiation results, 68 of 87 isolates (78.16%) was identifid as Pediococcus pentosaceus.
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Conclusion & Impact:The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effctive than the curing temperature.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two diffrent curing temperatures (4°C or 10°C) and two diffrent curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identifiation. According to the identifiation results, 68 of 87 isolates (78.16%) was identifid as Pediococcus pentosaceus. This species was followed by P. acidilactici (14.94%), Lactobacillus sakei (4.60%) and L. plantarum (2.30%), respectively. P. pentosaceus was dominant species in all curing applications (4°C/nitrate or nitrite or 10°C/nitrate or nitrite). Another species determined in all groups was P. acidilactici. While L. plantarum was only isolated from samples produced with nitrate (4°C or 10°C), L. sakei was isolated from samples produced with nitrite (4°C or 10°C). The effect of the curing agent on the biodiversity of lactic acid bacteria in pastirma was more effctive than the curing temperature.

Özet

İki farklı kürleme sıcaklığı (4°C veya 10°C) ve iki farklı kürleme ajanı (150 mg/kg sodium nitrit veya 300 mg/kg potasyum nitrat) kullanılarak kontrollü şartlar altında üretilen pastırmadan izole edilen laktik asit bakterileri, genotipik (16S rRNA) identifiasyona tabi tutulmuştur. İdentifikasyon sonuçlarına göre, 87 izolatın 68’i (%78.16) Pediococcus pentosaceus olarak tanımlanmıştır. Bu türü sırasıyla P. acidilactici (%14.94), Lactobacillus sakei (%4.60) ve L. plantarum (%2.30) takip etmiştir. Tüm kürleme uygulamalarında (4°C/nitrat veya nitrit veya 10°C/ nitrat veya nitrit), P. pentosaceus dominant türdür. Tüm gruplarda belirlenen diğer bir tür ise P. acidilactici’dir. L. plantarum yalnız nitrat (4°C veya 10°C) ile üretilen örneklerden identifiye edilirken, L. sakei yalnızca nitrit (4°C veya 10°C) kullanılarak üretilen örneklerde tanımlanmıştır. Pastırmada laktik asit bakterilerinin biyoçeşitliliği üzerine kürleme ajanının etkisi, kürleme sıcaklığınından daha etkili olmuştur.

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Cite This Article

ÇINAR, K., KABAN, G., FETTAHOĞLU, K. (2022). Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced with Diffrent Curing Processes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 25 - Sayı: 3, 299–303. https://ajindex.com/articles/569753

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