AI Summary & Key Takeaways
AI InsightResults: Apoptotic index in grade I EEC cell line among ganirelix-octreotide treated cells and leuprolide-octreotide combination therapy were respectively higher than the untreated control (p
📌 3 Key Takeaways
Abstract
Objective: The aim of this study was to compare apoptotic and antiproliferative effects of gonadotropin-releasing hormone analogues and their combination with octeotide on endometrioid endometrial cancer cell lines. Material and Method: Women diagnosed with endometrioid adenocarcinoma at the department of Gynecology and Obstetric of Kocaeli University Medical School were included in this research. Endometrium cancer cell lines obtained from three patients were used for this study. After trypsinization in 0.5% in calcium magnesium, free phosphate buffer solution (CMFPBS) cells were seeded on glass slides in 24-well plates containing DMEM-F12 medium and 10% fetal calf serum as culture medium. Cells were incubated for 24 hours at 37ºC in 5% CO2. GnRH agonist leuprolide (Lucrin 1 µmol/L), GnRH antagonist ganirelix (Orgalutran 1 µmol/L), leuprolide with octreotide (Sandostatin 10-6 mol/L), ganirelix with octreotide and no drug were added to the wells. Apoptosis and cells proliferations were evaluated after 12, 24, 48 and 72th hours of incubation. The percentage of apoptotic cells was evaluated by TdT mediated biotin-dUTP nick-end labeling (TUNEL) method; cell proliferation was assessed by bromodeoxyuridine (BrdU) incorporation. Results: Apoptotic index in grade I EEC cell line among ganirelix-octreotide treated cells and leuprolide-octreotide combination therapy were respectively higher than the untreated control (p
Özet
Amaç: Bu çalışmanın amacı endometriyal adenokanser hücre serilerinde GnRH analogları ve oktreotidin apoptozis ve hücre proliferasyonu üzerindeki etkilerini karşılaştırmaktır. Gereç ve Yöntemler: Kocaeli Üniversitesi Tıp Fakültesi Kadın hastalıkları ve Doğum bölümünde tanı almış olan endometriyal adenokanser vakaları çalışmaya alındı. Üç hastadan alınmış olan endometriyla kanser hücre serileri bu çalışmada kullanıldı. Tripinizasyon için %0.5 lik kalsiyum magnezyum, free phosphate buffer solusyonda (CMFPBS) bekletilen hücreler daha sonra DMEM-F12 mediyumu ve %10 dana fetus serumu içiren dishlere ekildi. Hücreler 37ºC in %5 CO2 de 24 saat inkübe edildiler. GnRH agonist leuprolide (Lucrin 1 μmol/L), GnRH antagonist ganirelix (Orgalutran 1 μmol/L), leuprolide ve octreotide (Sandostatin 10-6 mol/L), ganirelix ve octreotide ve ilaçsız gruplar oluşturuldu. Apoptozis ve hücre proliferasonu inkübasyondan sonraki 12, 24, 48 ve 72. saatlerde değerlendirildi. Apoptotik hücre oranı TUNEL yöntemi ile, hücre proliferasyonu ise bromodeoxyuridine (BrdU) ilavesiyle değerlendirildi. Bulgular: Grade I endometriyal kanser hücre serisindeki apoptotik index ganirelixoctreotide ve leuprolide-octreotide combination tedavileri alan gruplarda tedavisiz gruba göre anlamlı olarak daha yüksek olarak saptandı (p