AI Summary & Key Takeaways
AI InsightBothdoses of resveratrol treatment significantly decreased the number of TUNEL-positive andactive caspas-3-positive cells in all ipsi- and contralateral hippocampus and parietal cortexes.A significant difference was found between Resveratrol 50 and Resveratrol 100 mg/kg in allregions p( < 0.01).Conclusions: In conclusion, Resveratrol 100 mg/kg especially may be proposed as a highlypromising agent for preventing the unfavorable outcomes of traumatic brain damage in youngchildren.
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Abstract
Aim: Traumatic brain injury (TBI) is the leading cause of death and disability in childhood.The aim of the present study is to determine the acute effects of resveratrol (Res) in thehippocampus and parietal cortex in 7-day-old rat pups subjected to contusion injury.Methods: 28 pups were randomly allocated into four groups (per group, n= 7): control group(1), TBI group (2), TBI+50 mg/ kg resveratrol group (3) and TBI+100 mg/ kg resveratrolgroup (4). Single doses of resveratrol were injected intraperitoneally immediately after theinduction of TBI. The damage was examined by cresyl violet staining, terminaldeoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and active caspase- 3 assay 24 h after trauma.Results: Treatment with resveratrol significantly ameliorated the trauma induced neuron lossat the ipsi- and contralateral hippocampal brain regions and parietal cortex of rats, other thanthe CA2 and CA3 regions of the hippocampus in the TBI + 50 mg/ kg resveratrol group. Bothdoses of resveratrol treatment significantly decreased the number of TUNEL-positive andactive caspas-3-positive cells in all ipsi- and contralateral hippocampus and parietal cortexes.A significant difference was found between Resveratrol 50 and Resveratrol 100 mg/kg in allregions p( < 0.01).Conclusions: In conclusion, Resveratrol 100 mg/kg especially may be proposed as a highlypromising agent for preventing the unfavorable outcomes of traumatic brain damage in youngchildren.
Özet
Amaç: Travmatik beyin hasarı (TBH) çocukluk çağı ölüm ve sakatlıklarının önde gelennedenidir. Bu çalışmanın amacı, resveratrol kullanımının kontuzyon hasarı oluşturulan 7günlük sıçan hipokampus ve parietal korteksinde akut etkilerini belirlemektir.Method: 28 sıçan yavrusu rastgele dört gruba ayrıldı(her grupta n=7) kontrol grup (1), TBHgrup (2), TBH+50 mg/ kg resveratrol grup (3) and TBH+100 mg/ kg resveratrol grup (4).Resveratrol, TBH uygulamasından hemen sonra intraperitoneal olarak tek sefer uygulandı.Hasar, travmadan 24 saat sonra krezil viyole, deoxynucleotidyl transferase-mediated dUTPnick end labeling (TUNEL) ve active caspase-3 boyamaları ile incelendi.Bulgular: Resveratrol ile tedavi, TBH + 50 mg/ kg resveratrol grubunda, CA2 ve CA3bölgelerine göre hipokampusun diğer bölgelerinde ve parietal kortekste travmanın nedenolduğu nöron kaybını ipsilateral ve kontralateral olarak anlamlı derecede azalttı. Resveratroltedavisinin her iki dozu da ipsilateral ve kontralateral hipokampus ve parietal korteksteTUNEL- pozitif ve active caspase-3- pozitif hücre sayısını anlamlı olarak azalttı. Resveratrol50 and Resveratrol 100 mg/kg dozları arasında tüm bölgelerde anlamlı farklılık bulundu.p(