Demineralize Dana Omurgası Modeli Osteoporoz Araştırmalarında Kullanılabilirmi?

As a result, bone mineral density changes was statistically significant betweentwo group, but histological appearance was far from ordinary bone with empty bone lacunesin demineralized vertebras.

📌 3 Key Takeaways

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Objective & Hypothesis:In osteoporosis researchs, animal models have been used widely, but every model haveassociated with some advantages and disadvantages.

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Key Findings & Data:Than,demineralization procedure was done for each L1 and L5 vertebras of calves whereas othervertebras were used as control group.

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Conclusion & Impact:As a result, bone mineral density changes was statistically significant betweentwo group, but histological appearance was far from ordinary bone with empty bone lacunesin demineralized vertebras.

🔬Design:Modeling & Simulation

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

In osteoporosis researchs, animal models have been used widely, but every model haveassociated with some advantages and disadvantages. In vitro studies with fresh cadaver looklike good solution, however paucity of cadaver restricts its use. Demineralized calf vertebramodel have been introduced as a new option. The aim of present study to check this newmodel on histopathological view and bone mineral density changes. Lumbar spines of sevenfresh calves were cleaned from soft tissues and were submitted to DXA exams which providethe areal bone mineral density (BMD ) and bone mineral contents ( BMC ). Than,demineralization procedure was done for each L1 and L5 vertebras of calves whereas othervertebras were used as control group. Following procedure, new DEXA exams were made.Light microscobic evaluation with Hematoxylen-Eosine staining was achieved in control andstudy groups. As a result, bone mineral density changes was statistically significant betweentwo group, but histological appearance was far from ordinary bone with empty bone lacunesin demineralized vertebras.

Özet

Osteoporoz araştırmalarında hayvan modelleri geniş olarak kullanılmaktadır fakat hermodelin avantajları ve dezavantajları bulunmaktadır. Taze kadavrayla yapılan in vitroçalışmalar iyi sonuçlar vermektedir, bununla beraber kadavra teminideki problemlerkullanımını zorlaştırmaktadır. Demineralize dana vertebra modeli yeni bir seçenek olabilir.Bu çalışmanın amacı bu yeni modelde histopatoloji ve mineral dansitesindeki değişiklikleriincelemektir. Yedi adet taze dana omurgasının yumuşak dokuları temizlendi ve DEXA ilekemik mineral miktarları ve mineral dansiteleri ölçüldü. Demineralizasyon her omurgada L1ve L5 vertebralarına uygulandı. Diğer vertebralar kontrol grubu olarak kullanıldı. İşlemdensonra yeni DEXA ölçümleri yapıldı. Işık mikroskobunda hematoksilen eozin boyamasıkullanılarak histolojik inceleme yapıldı. Sonuç olarak kemik mineral dansiteleri iki gruparasında istatistiki olarak farklı bulundu. Demineralize vertebralardaki boş kemik lakünleriylenormal kemik yapıları histolojik olarak birbirinden farklı bulundu.

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Cite This Article

EREL, N., AKÇAY, E., AYDIN, T., DEMİRCİVİ, F. O., CAMLAR, M., GÖKKARA, T., YAGCI, A., AYDIN, M. (2022). Demineralize Dana Omurgası Modeli Osteoporoz Araştırmalarında Kullanılabilirmi?. Journal of Neurological Sciences (Turkish), Cilt: 31 - Sayı: 4, 680–686. https://ajindex.com/articles/565060

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References (15)

Akbay A, Bozkurt G, Ilgaz Ö, Palaoğlu S, Akalan N, Benzel EC. A demineralized calf vertebra model as an alternative to classic osteop orotic vertebra models f or p edicle screw p ullout studies Eur Sp ine J. 2008; 17(3): 468- 473
Cotterill PC,Kostvik JP,D 'Angelo G,Fernie GR, Makı BE. An anatomical comp arison of the human and bovine thoracolumbar sp ine J Orthop Res. 1986; 4(3): 298-303
Jee WSS, Yao W. Overview: animal models of osteop enia and osteop orosis. J Musculoskelet Neuronal Interact.2001; 1(3): 193-207
Kaveh K, İbrahim R,AbuBakar MZ, İbrahim TA, Osteop orosis induction in animal model American J Animal & Vet Sci. 2010; 5(2): 139- 145
Lelovas PP, Xanthos T, Thoma SE, Lyritis GP, Dontas IA. The laboratory rat as an animal model f or osteop orosis research Comp Med. 2008; 58(5): 424-430
Lewandrowsky K.-U, Venugop alan V, Tomf ord WW, Schomacker KT, Mankin HJ, Deutsch TF. Kinetics of cortical bone demineralization:controlled demineralization-a new method f or modifyingcortical bone allograf ts. J Biomed Mat Res. 1996; 31(3): 365-372
Mainil-Varlet P. Bone p athology in exp erimental osteop orosis: a review J Ortop h Sci.1997; 2: 185- 190
Manolagas SC. Bırth and death of bone cells: basic regulatory mechanisms and imp lications f or the p athogenesis and treatment of osteop orosis Endocr Rev. 2000; 21(2): 115- 137
Minaire P, Immobilization osteop orosis: a review Clin Rheumatol. 1989; 8(2): 95-103
Miyakoshi N, Sato K, Abe T,Tsuchida T,Tamura Y, Kudo T. Histomorp hometric evaluation of the eff ects of ovariectomy on bone turnover in rat caudal vertebrae. Calcif Tissue Int. 1999; 64: 318-324
Sheng S-R, Wang X-Y,Xu H-Z,Zhu G-Q,Zhou Y- F. Anatomy of large animal sp ines and its comp arison to the human sp ine: a systematic review Eur Sp ine J. 2010; 19(1): 46-56
Swartz DE, Wittenberg RH,Shea M, White AA3rd,Hayes WC.Physical and mechanical p rop erties of calf lumbosacral trabecular bone. J Biomech. 1991; 24(11): 1059-1068
Turner RT,Maran AM,Lotunin S, Heff era T,Evans GL,Zhang M,Sibonga JD. Animal model of osteop orosis. Rev Endocr Metab Disord.,2001; 2(1): 117-127
Xia X,Kan R,Gluhak-Heinrich J,Yao W,Lane NE, Bonewald LF, Biswas SK, Lo W-K,Jian JX. Glucocorticoid-induced autop hagy in osteocytes. J Bone Miner Res. 2010; 25(11): 2479-2488
Zarrinkalam MR,Mulaibrahimoviç A, Atkins GJ, Moore RJ. Changes in osteocyte density corresp ond with changes in osteoblast and osteo