Journals / Journal of Istanbul University Faculty of Dentistry / 2015 / Cilt: 49 - Sayı: 1

Çeşitli İrrigasyon Solüsyonlarının İnsan Kök Kanalındaki Staphylococcus aureus'a Karşı Antibakteriyel Etkileri

BACTERICIDAL EFFECTS OF VARIOUS IRRIGATION SOLUTIONS AGAINST STAPHYLOCOCCUS AUREUS IN HUMAN ROOT CANAL

Pages
19–26
Publication Date
2022-10-18
DOI
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Results: Although there were statistically significant differences among SPO and other experimental groups (p0.05).Conclusion: Super-oxidized water may be recommended as an alternative irrigation solution instead of NaOCl against S. aureus in root canals.

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Objective & Hypothesis:Purpose: The aim of the present study was to evaluate and to compare the antibacterial effects of various irrigation solutions against Staphylococcus aureus (S. aureus) in human root canals.Materials and Methods: 120 single-root mandibular premolar teeth were selected.
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Key Findings & Data:S. aureus was incubated in the root canals and kept at 37°C for 24h.
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Conclusion & Impact:Results: Although there were statistically significant differences among SPO and other experimental groups (p0.05).Conclusion: Super-oxidized water may be recommended as an alternative irrigation solution instead of NaOCl against S. aureus in root canals.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Purpose: The aim of the present study was to evaluate and to compare the antibacterial effects of various irrigation solutions against Staphylococcus aureus (S. aureus) in human root canals.Materials and Methods: 120 single-root mandibular premolar teeth were selected. The teeth were prepared and sterilized. S. aureus was incubated in the root canals and kept at 37°C for 24h. The infected root canals were divided into one positive (saline) and one negative (sodium hypochlorite) control, and four experimental groups [Ethylene-diaminetetraaceticacid, Chlorhexidine Gluconate, Super-oxidized water(SPO), Aqueous ozone] (n=20). Flow rate of irrigation was applied with 5 mL/min flow rate for 3 min to ensure standardization among all study groups. Following the irrigation, paper points were placed in the root canals and then transferred in sterile eppendorf. Remaining bacteria were counted on blood agar plates and the data were analyzed using one-way ANOVA and Tukey's test. Results: Although there were statistically significant differences among SPO and other experimental groups (p0.05).Conclusion: Super-oxidized water may be recommended as an alternative irrigation solution instead of NaOCl against S. aureus in root canals.

Özet

Amaç: Bu çalışmanın amacı, insan kök kanallarında Staphylococcus aureus (S. aureus)'a karşı çeşitli irrigasyon solüsyonlarının antibakteriyel etkilerini değerlendirmek ve karşılaştırmaktır.Gereç ve Yöntem: 120 tek kök mandibular premolar dişler seçildi. Dişler genişletildi ve sterilize edildi. S. aureus kök kanalı içinde inkübe edildi ve 24 saat boyunca 37°C'de tutuldu. Enfekte kök kanalları bir pozitif (serum fizyolojik) ve bir negatif (sodyum hipoklorit) kontrol ve dört deney grubuna (Etilen diamin tetra asetik asit, Chlorhexidine Gluconate, Super-oxidized water, Aqueous ozone) ayrıldı (n=20). Irrigasyon akış hızı, tüm çalışma grupları arasında standardizasyonu sağlamak amacıyla 5 mL/dakika akış hızıyla 3 dakika boyunca uygulandı. Irrigasyonu takiben kağıt koniler kök kanalı içine yerleştirildi ve daha sonra steril eppendorf tüplerine aktarıldı. Kalan bakteriler kanlı agar plakaları üzerinde sayıldı ve veriler tek yönlü ANOVA ve Tukey testi kullanılarak analiz edildi.Bulgular: Süper-okside su ile diğer deney grupları arasında istatistiksel olarak anlamlı fark olmasına rağmen (p0,05). Sonuç: Süper-okside su, kök kanallarındaki S. aureus'a karşı NaOCl'ye alternatif bir irrigasyon solüsyonu olarak tavsiye edilebilir.

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Cite This Article

HUBBEZOĞLU, İ., TUNÇ, T., ZAN, R., KUTLU, G., MUTLU, Z., SÜMER, Z. (2022). Çeşitli İrrigasyon Solüsyonlarının İnsan Kök Kanalındaki Staphylococcus aureus'a Karşı Antibakteriyel Etkileri. Journal of Istanbul University Faculty of Dentistry, Cilt: 49 - Sayı: 1, 19–26. https://ajindex.com/articles/563850

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References (13)

  1. Harrison JW. Irrigation of the root canal system. Dent Clin North Am 1984;28(4):797-808.
  2. Fidalgo TK, Barcelos R, Portela MB, Soares RM, Gleiser R, Silva-Filho FC. Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus. Braz Oral Res 2010;24(4):406-412.
  3. Clarkson MR, Moule JA. Sodium hypochlorite and its use as an endodontic irrigant. Aust Dent J 1998;43(4):250-256.
  4. Turkun M, Cengiz T. The effects of sodium hypochlorite and calcium hydroxide on tissue dissolution and root canal cleanliness. Int Endod J 1997;30(5):335-342.
  5. Heling I, Chandler NP. Antimicrobial effect of irrigant combinations within dentinal tubules. Int Endod J 1998;31(1):8-14.
  6. Yamada RS, Armas A, Goldman M, Lin PS. A scanning electron microscopic comparison of a high volume final flush with several irrigating solutions: Part 3. J Endod 1983;9(4):137-142.
  7. Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol 1990;6(4):142-149.
  8. Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004;30(11):778-781.
  9. Fischer P, Thofern E, Botzenhart K. Vergleichende untersuchungen zur wirksamkeit von chlor und ozon auf bakterien und sporen. Zentralbl Bakteriol Orig B 1978;166(4-5):399-407. Huth KC1, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, Hickel R, Brand K. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006;114(5):435-440.
  10. McDonnell WF, Horstman DH, Hazucha MJ, Seal E Jr, Haak ED, Salaam SA, House DE. Pulmonary effects of ozone exposure during exercise: dose-response characteristics. J Appl Physiol 1983;54(5):1345-1352.
  11. Nogales CG, Ferari PH, Kantorovich EO, Lage- Marques JL. Ozone Therapy in Medicine and Dentistry. J Contemp Dent Pract 2008;9(4):75- 84. Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1998;31(1):1-7.
  12. Siren EK, Haapasalo MP, Ranta K, Salmi P, Kerosuo EN. Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation. Int Endod J 1997;30(2):91-95.
  13. Stoll R, Venne L, Jablonski-Momeni A, Mutters R, Stachniss V. The disinfecting effect of ozonized oxygen in an infected root canal: an in vitro study. Quintessence Int 2008;39(3):231-236. Cardosa MG, de Oliveira LD, Koga-Ito