Journals / Journal of FisheriesSciences.com / 2009 / Cilt: 3 - Sayı: 4

The recent methods on using for purification of fish proteins

Balık proteinlerinin saflaştırılmasında kullanılan son yöntemler

Pages
298–309
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In recent years, the studies about using of the pure fish proteins to increase the nutritional value in the functional foods have been carried out.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, we have reviewed that the methods have been used to purification of fish proteins in the recent years.
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Key Findings & Data:These methods; the chromatographic (gel filtration, ion exchange, affinity, reversed phase, hidrofobic interaction, membrane and fast protein liquid chromatographies- FPLC-) and electrophoretic (isoelectric focusing, sodium dodecyl sulfate polyacrylamide gel electrophoresis-SDS-PAGE-, capillary electrochromatography).
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Conclusion & Impact:In recent years, the studies about using of the pure fish proteins to increase the nutritional value in the functional foods have been carried out.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, we have reviewed that the methods have been used to purification of fish proteins in the recent years. These methods; the chromatographic (gel filtration, ion exchange, affinity, reversed phase, hidrofobic interaction, membrane and fast protein liquid chromatographies- FPLC-) and electrophoretic (isoelectric focusing, sodium dodecyl sulfate polyacrylamide gel electrophoresis-SDS-PAGE-, capillary electrochromatography). Intended pure proteins which have been selected according to the characteristics of physical, chemical and biological could be purified and isolated by one or multiple of these methods. The maximum protein is obtained with the membrane chromatography, minimum protein is obtained with the affinity chromatography. In recent years, the studies about using of the pure fish proteins to increase the nutritional value in the functional foods have been carried out.

Özet

Bu çalışmada, son yıllarda balık proteinlerinin saflaştırılması için kullanılan yöntemler incelenmiştir. İncelenen kromatografik yöntemler; jel filtrasyon kromatografisi, iyon değişim kromatografisi, affinite kromatografisi, revers faz kromatografisi, hidrofobik etkileşim kromatografisi, membran kromatografisi, hızlı performans likit kromatografisi (FPLC) ve elektroforetik yöntemler ise; izoelektrik fokuslama, sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE), kapiller elektroforezdir. Kullanılacak olan yöntemler saflaştırılması arzu edilen proteinin fiziksel, kimyasal ve biyolojik özellikleri göz önüne alınarak seçilmektedir. Proteini, en saf halde elde edebilmek için bu yöntemlerin çoğu ardışık olarak uygulanmaktadır. En fazla miktarda protein, membran kromatografisiyle, en az miktarda protein, affinite kromatografisi ile elde edilmektedir. Son yıllarda, elde edilen saf proteinler, birçok alanda kullanıma sunulmakta ve bu alan hızla gelişen bir sektör olarak diğer sektörler arasında yerini almaktadır.

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Cite This Article

ÇAĞATAY, T., ÜNLÜSAYIN, M., ERDİLAL, R. (2022). The recent methods on using for purification of fish proteins. Journal of FisheriesSciences.com, Cilt: 3 - Sayı: 4, 298–309. https://ajindex.com/articles/563303

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