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Journals / Turkish Journal of Veterinary and Animal Sciences / 2006 / Cilt: 30 - Sayı: 1

Characterization of Footrot Bacteria Dichelobacter nodosus Using PCR Amplification and DNA Sequence Analysis

İfakat Tülay ÇAĞATAY
Jon G. H. HICKFORD
Pages
53–59
Publication Date
2006-01-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank (NCBI) and commercial vaccine strains.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle.
📊
Key Findings & Data:In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ).
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Conclusion & Impact:These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank (NCBI) and commercial vaccine strains.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Footrot · Dichelobacter nodosus · fimA gene

Abstract

Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ). Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene (fimA) were amplified from bacterial DNA using polymerase chain reaction (PCR). On the basis of PCR and DNA sequencing, 6 new D. nodosus isolates were identified and characterized from NZ. These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank (NCBI) and commercial vaccine strains.

Özet

Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ). Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene (fimA) were amplified from bacterial DNA using polymerase chain reaction (PCR). On the basis of PCR and DNA sequencing, 6 new D. nodosus isolates were identified and characterized from NZ. These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank (NCBI) and commercial vaccine strains.

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Cite This Article

ÇAĞATAY, İ. T., HICKFORD, J. G. H. (2006). Characterization of Footrot Bacteria Dichelobacter nodosus Using PCR Amplification and DNA Sequence Analysis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 30 - Sayı: 1, 53–59. https://ajindex.com/articles/122488

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References (7)

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