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Journals / Journal of Clinical and Analytical Medicine / 2015 / Cilt: 6 - Sayı: 3

Eritropoetinin gürültü uygulaması sonrası kokleada S100 proteini ekspresyonuna etkisi: Deneysel çalışma

The effect of erythropoietin on S100 protein expression in cochlea after acoustic overstimulation: An experimental study

Hatice Efsun KOLATAN
Görkem ESKİİZMİR
Günay KIRKIM
Seren Gülşen GÜRGEN
Oğuzhan GÜRGEN
Selhan GÜRK
Pages
304–308
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

S100 staining demonstratedstrong reaction in Schwann cells and myelin sheaths of SGNs in healthy con- trol group (p

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: To investigate the efect of Erythropoietin on acoustically overstimu- ated rat spiral ganglion neurons (SGNs) using S100 protein immunostaining.
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Key Findings & Data:Material and Method: Twenty-two Wistar albino rats were divided into threegroups: healthy control group (n=7), Saline solution (n=7) and Erythropoietinnjection groups (n=8).
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Conclusion & Impact:S100 staining demonstratedstrong reaction in Schwann cells and myelin sheaths of SGNs in healthy con- trol group (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: To investigate the efect of Erythropoietin on acoustically overstimu- ated rat spiral ganglion neurons (SGNs) using S100 protein immunostaining. Material and Method: Twenty-two Wistar albino rats were divided into threegroups: healthy control group (n=7), Saline solution (n=7) and Erythropoietinnjection groups (n=8). Saline solution and Erythropoietin injection groups re- ceived white noise (100 dB SPL) for 3 hours. Cochlear sections were stainedby silver staining technique and immunostained by S100 antibody. Results:Histochemical analysis of silver staining sections revealed normal structureand a weak staining in SGNs of healthy control group. However, dark-blackcytoplasmic staining, cellular shrinkage and degeneration were detectedn saline injection group. On the other hand, a few weakly stained neuronswere observed in erythropoietin injection group. S100 staining demonstratedstrong reaction in Schwann cells and myelin sheaths of SGNs in healthy con- trol group (p

Özet

Amaç: Çalışmamızda Eritropoetinin, gürültü ile uyarılmış sıçan spiral gangion nöronlarında S100 proteini dağılımına etkisinin, immunohistokimyasalyöntem ile araştırılması amaçlandı. Gereç ve Yöntem: Yirmi-iki Wistar albino sıçan üç gruba ayrıldı: sağlıklı kontrol grup (n=7), Serum fizyolojik enjeksiyon grubu (n=7) ve Eritropoetin enjeksiyon grubu (n=8). Serum fizyolojik veEritropoetin enjeksiyon gruplarına 3 saat süre ile beyaz gürültü (100 dB SPL)uygulandı. Koklea kesitleri gümüşleme yöntemi ile histokimyasal olarak veS100 antikoru ile immunohistokimyasal olarak boyandı. Bulgular: Gümüşleme yöntemine göre sağlıklı kontrol grubu spiral ganglion nöronlarında normalyapı ve zayıf bir boyanma gözlendi. Serum fizyolojik enjeksiyon grubu ganglion nöronlarının sitoplazmasında koyu-siyah boyanma ve dejeneratif görünümbelirlendi. Eritropoetin enjeksiyon grubunda ise az sayıda nöronun koyu boyandığı izlendi. S100 immunohistokimyasal boyamasına göre, sağlıklı kontrol grubunda Schwann hücrelerinde ve myelin lamellerde kuvvetli reaksiyongözlendi (p

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Cite This Article

KOLATAN, H. E., ESKİİZMİR, G., KIRKIM, G., GÜRGEN, S. G., GÜRGEN, O., GÜRK, S. (2022). Eritropoetinin gürültü uygulaması sonrası kokleada S100 proteini ekspresyonuna etkisi: Deneysel çalışma. Journal of Clinical and Analytical Medicine, Cilt: 6 - Sayı: 3, 304–308. https://ajindex.com/articles/560986

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