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Journals / Journal of Clinical and Analytical Medicine / 2016 / Cilt: 7 - Sayı: 6

Yaşlanmayla Sıçan Kemiğindeki Aquaporin-1 ve Aquaporin-3 Ekspresyonu Değişikliklerinin Araştırılması

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Kürşad AYTEKİN
Murat UYSAL
Seda OCAKLI
Hüseyin ASLAN
Murat ZÜMRÜT
Hilal Irmak SAPMAZ
Ufuk TAŞ
Sabiha Kübra ALICI
Pages
807–811
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Discussion: Our findings suggest that reduced amounts of AQP1 and AQP3 may be related to agerelated changes in bone tissue.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Aquaporins are membrane channel proteins that transport water and ions.
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Key Findings & Data:The amount of aquaporin-1 (AQP1) and aquaporin-3 (AQP3) is considered to decrease in cartilage tissue with age.
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Conclusion & Impact:Discussion: Our findings suggest that reduced amounts of AQP1 and AQP3 may be related to agerelated changes in bone tissue.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: Aquaporins are membrane channel proteins that transport water and ions. The amount of aquaporins in tissue changes with age. The amount of aquaporin-1 (AQP1) and aquaporin-3 (AQP3) is considered to decrease in cartilage tissue with age. However, no age-related change has been reported regarding the amount of aquaporins in bone tissue. In this study, our aim was to examine expressions of AQP1, AQP3, and type I collagen immunohistochemically in the bone tissues of young and old rats. Material and Method: Fourteen Wistar Albino rats were included in this study. Group I (n=7) consisted of young rats that were two months old, while Group II (n=7) consisted of old rats that were eighteen months old. Bone tissue (femur) was dissected and examined histopathologically and immunohistochemically. After routine histological procedure, sections at 4-5 µm thickness were obtained from tissues embedded in paraffin. Sections were stained immunohistochemically for AQP1, AQP3, and type I collagen as well as with hematoxylin-eosin. Results: Immunohistochemically, expressions of AQP1, AQP3, and type I collagen were demonstrated in both young and old rats. AQP1, AQP3, and type I collagen amounts were found to decrease with aging. Discussion: Our findings suggest that reduced amounts of AQP1 and AQP3 may be related to agerelated changes in bone tissue.

Özet

Amaç: Aquaporinler su ve iyon transportu sağlayan membran kanal proteinleridir. Yaşlanma ile dokulardaki aquaporinlerin miktarı değişmektedir. Aquaporin-1 (AQP1) ve aquaporin-3 (AQP3)'ün kıkırdak dokuda yaşlanma ile azaldığı gösterilmiştir. Ancak kemik dokuda yaşlanma ile aquaporinlerin miktarında değişim gösterilmemiştir. Bu çalışmada genç ve yaşlı hayvanların kemik dokularında AQP1, AQP3 ve tip I kollajen ekspresyonlarının immünohistokimyasal olarak araştırılması amaçlandı. Gereç ve Yöntem: Bu çalışmada 14 adet Wistar Albino cinsi sıçan kullanıldı. Grup I (n=7) iki aylık genç sıçanlardan, Grup II (n=7) onsekiz aylık yaşlı sıçanlardan oluşmaktaydı. Kemik dokusu (femur) histopatolojik ve immunohistokimyasal değerlendirme için alındı. Rutin histolojik prosedürün ardından parafine gömülen dokulardan 4-5 µm kalınlığında kesitler alındı. İmmunohistokimyasal olarak AQP1, AQP3 ve tip I kollajen boyama ayrıca Hematoksilen-eozin boyama yapıldı. Bulgular: Genç ve yaşlı sıçanlarda immünohistokimyasal olarak AQP1, AQP3 ve tip I kollajen ekspresyonu gösterildi. Yaşlanma ile AQP1, AQP3 ve tip I kollajen miktarında azalma olduğu gözlendi. Tartışma: AQP1 ve AQP3 mik tarındaki azalmanın kemik dokuda yaşa bağlı değişikliklerle ilgili olabilece ği kanaatindeyiz

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Cite This Article

AYTEKİN, K., UYSAL, M., OCAKLI, S., ASLAN, H., ZÜMRÜT, M., SAPMAZ, H. I., TAŞ, U., ALICI, S. K. (2022). Yaşlanmayla Sıçan Kemiğindeki Aquaporin-1 ve Aquaporin-3 Ekspresyonu Değişikliklerinin Araştırılması. Journal of Clinical and Analytical Medicine, Cilt: 7 - Sayı: 6, 807–811. https://ajindex.com/articles/560863

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