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Journals / Journal of Cell and Molecular Biology / 2003 / Cilt: 2 - Sayı: 1

Arabidopsis thaliana' nın col-5 ekotipinden elde edilen mutant hatlardan RPP7 geninin karakterizasyonu

Characterisation of RPP7 mutant lines of the col-5 ecotype of Arabidopsis thaliana

Canan CAN
Eric B. HOLUB
Mehmet ÖZASLAN
Pages
25–30
Publication Date
2022-10-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that the RPP7gene present in the Col-5 ecotype is a unique gene different from the other RPP genes present in Col-5.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, phenotypic characterization of RPP7that confers resistance to Hiks1isolate of Peronospora parasitica, deficient mutant lines of Col-5 ecotype of Arabidopsis thalianawas investigated.
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Key Findings & Data:The Col-5 plants that exposed to Fast Neutron (FN) were inoculated with 8 different P.
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Conclusion & Impact:It was found that the RPP7gene present in the Col-5 ecotype is a unique gene different from the other RPP genes present in Col-5.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, phenotypic characterization of RPP7that confers resistance to Hiks1isolate of Peronospora parasitica, deficient mutant lines of Col-5 ecotype of Arabidopsis thalianawas investigated. The Col-5 plants that exposed to Fast Neutron (FN) were inoculated with 8 different P. Parasitica isolates and symptom development was investigated. A total of 4 mutant lines were analyzed. It was found that the RPP7gene present in the Col-5 ecotype is a unique gene different from the other RPP genes present in Col-5.

Özet

Bu çalışmada, Arabidopsis thaliana’nın Col-5 ekotipinde bulunan ve Peronospora parasitica’nın Hiks-1 izolatına karşı dayanıklılığı sağlayan RPP7 geninde mutasyon içeren hatların fenotipik olarak belirlenmesi üzerinde araştırmalar gerçekleştirilmiştir. Fast Nötron (FN) uygulamaları ile mutasyon meydana getirilmiş Col-5 tohumlarından gelişen bitkiler 8 farklı P. parasitica izolatı ile inokule edilerek semptom gelişimleri incelenmiştir. Toplam olarak 4 mutant hatta gerçekleştirilen analizlerde, RPP7 geninin Col-5 ekotipinde bulunan ve farklı P. parasitica izolatlarına karşı dayanıklılığı sağlayan genlerden bağımsız olarak fonksiyon gösteren bir gen olduğu belirlenmiştir.

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Cite This Article

CAN, C., HOLUB, E. B., ÖZASLAN, M. (2022). Arabidopsis thaliana' nın col-5 ekotipinden elde edilen mutant hatlardan RPP7 geninin karakterizasyonu. Journal of Cell and Molecular Biology, Cilt: 2 - Sayı: 1, 25–30. https://ajindex.com/articles/560715

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References (10)

  1. Aarts N, Metz M, Holub E, Staskawicz BJ, Daniels M and Parker JE. Differential requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated pathways in Arabidopsis. Proc Natl Acad Sci USA. 95: 10306-10311, 1998.
  2. Ausubel F, Brent R, Kingston RE, Moore DA, Seiddman JG, Smith JA and Struhl K. Current Protocols in Molecular Biology. John Wiley and Sons. 1994.
  3. Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmidt R, Giraudat J, Leung J and Staskawicz BJ. RPS2 of Arabidopsis thaliana, A leucine-rich repeat class of plant disease resistance genes. Science. 265 (5180): 1856-1860, 1994.
  4. Bittner-Eddy PD, Crute IR, Holub EB and Beynon JL. RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify downy mildew resistance to different avirulence determinants in Peronospora parasitica. The Plant Journal. 21(2): 177-188, 2000. Bittner-Eddy PD and Beynon JL. The Arabidopsis downy mildew resistance gene, RPP13-Nd, functions independently of NDR1 and EDS1 and does not require the accumulation of salicylic acid. Mol Plant-Microbe Interactions. 14: 416-421, 2001.
  5. Botella MA, Parker JE, Frost LN, Bittner-Eddy PD, Beynon JL, Daniels MJ, Holub EB and Jones JDG. Three genes of the Arabidopsis RPP1 complex resistance locus recognize distinct Peronospora parasitica avirulence determinants. Plant Cell. 10: 1847-1860, 1998.
  6. Can C. Bittner-Eddy P, Tör M, Williams K, Gunn N, Bakht S, Atkinson L, Debener T, Chimot P, Crute I, Beynon J and Holub EB. Revealing the organization of RPP loci in the Arabidopsis thaliana genome, Poster abstract in 6th International Conference on Arabidopsis Research, Medison, Winconsin. 7-11 June, 1995. Can C. Genomic organisation of pathogen recognition genes in Arabidopsis thaliana to Peronospora parasitica, Ph. D. Thesis, University of London, Wye Collage, UK. 1997.
  7. Century KS, Holub EB and Staskawicz BJ. NDR1, a locus of Arabidopsis thaliana that is required for disease resistance both a bacterial and a fungal pathogen. P roc Natl Acad Sci USA. 92 (14): 6597-6601, 1995. Dangl JL and Jones JDG. Plant pathogens and integrated defense responses to infection. Nature. 411: 826-833, 2001.
  8. Dietrich RA, Richberg MA, Schmidt R, Dean C and Dangl JL. A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell. 88: 685-694, 1994.
  9. Dong X. SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol. 1: 316-323, 1998.
  10. Falk A, Feys BJ, Frost LN,