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Journals / Journal of Basic and Clinical Health Sciences / 2019 / Cilt: 3 - Sayı: 2

Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells

Yasemin BAŞBINAR
Hanife Ecenur MEÇO
Türkan YİĞİTBAŞI
Ezgi DAŞKIN
Ahu PAKEMİRLİ
Tuğba UYSAL
Caner KARACA
Hülya ELLİDOKUZ
Pages
83–88
Publication Date
2022-10-19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Mann Whitney U Test was used to compare independentsamples.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malignphenotypes.
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Key Findings & Data:Cross-talk via soluble factors also shifts the metastatic character.Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in3-dimensional (3D) in vitro cancer model is analyzed.
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Conclusion & Impact:Mann Whitney U Test was used to compare independentsamples.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malignphenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bonemarrow-derived mesenchymal stem cells (BM-MSCs) to have this ability due to cell-cell interaction, released cytokines, and organization withthe extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character.Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in3-dimensional (3D) in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3Dstructure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects. The invasion andmigration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independentsamples. All P-values

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Cite This Article

BAŞBINAR, Y., MEÇO, H. E., YİĞİTBAŞI, T., DAŞKIN, E., PAKEMİRLİ, A., UYSAL, T., KARACA, C., ELLİDOKUZ, H. (2022). Limitations of Cross-Talk Between Osteosarcoma and Bone Marrow-Derived Mesenchymal Stem Cells. Journal of Basic and Clinical Health Sciences, Cilt: 3 - Sayı: 2, 83–88. https://ajindex.com/articles/560596

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