miRNA-129-3P Expression in Synovial Fluid of Patients with Osteoarthritis

Objective & Hypothesis:Objective: The aim of this study was to investigate the miRNA-129-3p expressions in the synovial fluid of patients with a primary knee osteoarthritis (OA), and thereby contribution to the elucidating underlying molecular mechanisms in OA pathophysiology.

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Key Findings & Data:Methods: Patient group included 31 individuals with an advanced knee OA.

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Conclusion & Impact:Statistical significance was set at p

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study was to investigate the miRNA-129-3p expressions in the synovial fluid of patients with a primary knee osteoarthritis (OA), and thereby contribution to the elucidating underlying molecular mechanisms in OA pathophysiology. Methods: Patient group included 31 individuals with an advanced knee OA. A total of 13 patients with anterior cruciate ligament rupture who had no cartilage damage, were chosen as a control group. Synovial fluid samples were collected during the total knee arthroplasty and an arthroscopic reconstruction. After the centrifugation, samples were stored in a -80 °C cooler. The miRNA-129-3p expressions were examined by reverse transcriptase-polymerase chain reaction using the RNU44 molecule as a reference. The 2-ΔΔCt method was used to calculate the expression of molecules. Statistical comparisons were undertaken by the Student’s t-test. Pearson’s chi-square test was used to determine the differences in the ratios or relationships between the categorical variables. Statistical significance was set at p

Özet

Amaç: Bu çalışmanın amacı, primer diz osteoartriti (OA) olan hastaların sinovyal sıvılarındaki miRNA-129-3p ekspresyonlarını araştırmak ve böylelikle OA patofizyolojisinde altta yatan moleküler mekanizmaların aydınlatılmasına katkıda bulunmaktır. Yöntemler: Hasta grubu ilerlemiş diz OA’sı olan 31 kişiden oluşuyordu. Kontrol grubu olarak kıkırdak hasarı olmayan ön çapraz bağ rüptürü olan 13 hasta seçildi. Total diz artroplastisi ve artroskopik rekonstrüksiyon sırasında sinoviyal sıvı örnekleri toplandı. Santrifüjden sonra numuneler -80 °C’lik bir soğutucuda saklandı. miRNA-129-3p ekspresyonları, ters transkriptaz-polimeraz zincir reaksiyonu ile incelendi. RNU44 molekülü referans olarak kullanıldı. Moleküllerin ekspresyonunu hesaplamak için 2-ΔΔCt yöntemi kullanıldı. İstatistiksel karşılaştırmalar Student’s t-testiyle yapıldı. Kategorik değişkenler arasındaki oranlar veya ilişkilerdeki farklılıkları belirlemek için Pearson ki-kare testi kullanıldı. Tüm olgular için istatistiksel anlamlılık p

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Cite This Article

UZUN, H., POLAT, S. H. B., POLAT, A., ŞAHİN, G., DARIYERLİ, N. (2022). miRNA-129-3P Expression in Synovial Fluid of Patients with Osteoarthritis. JAREM, Cilt: 12 - Sayı: 1, 1–4. https://ajindex.com/articles/558138

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