AI Summary & Key Takeaways
AI InsightThe percentage of TO+ platelets in 58 control subjects was %3.85 (1.99 (±SD In 27 thrombocytopenic patients whose bone marrow contained normal to increased number of megakaryocytes, the percentage of TO+ platelets %16.93 12.93 (±SD was significantly elevated (p
📌 3 Key Takeaways
Abstract
Thiazole Orange (TO), a fluorescent dye originally used for reticulocyte analysis, is characterized by a large fluorescence enhancement yield on binding to nucleic acids, particularly RNA. We applied TO staining to platelets in whole blood samples from haematologically normal subjects and patients with various quantitative platelet disorders. The percentage of TO+ platelets in 58 control subjects was %3.85 (1.99 (±SD In 27 thrombocytopenic patients whose bone marrow contained normal to increased number of megakaryocytes, the percentage of TO+ platelets %16.93 12.93 (±SD was significantly elevated (p
Özet
Thiazole orange (TO) nükleik asitlere, özellikle RNA ya bağlandığında fluoresans oluşturan, aslında retikülosit sayımında kullanılmış bir boyadır. TO boyamasını hematolojik olarak normal kişilerde ve çeşitli niceliksel trombosit bozukluğu olan hastalarda tam kan örneklerinde kullandık. 58 kontrol örneğinde TO ile boyanan (TO+) trombositlerin oranı %3.85±1.99(±SS) bulundu. Kemik iliğinde megakaryosit sayısı normal veya artmış olan 27 trombositopenik hastada TO+ trombosit oram %16.93+12.93 bulundu. Bu kontrole göre anlamlı olarak yüksekti (p