Journals / İnönü Üniversitesi Tıp Fakültesi Dergisi / 2010 / Cilt: 17 - Sayı: 3

The histological evaluation of the testes of rats which applied formaldehyde and lavender oil

Solunum yoluyla formaldehit ve lavanta uygulanan sıçan teslislerinin değerlendirilmesi; bir histolojik çalışma

Pages
169–173
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It was shown that harmful effects of FA on testes occured via formaldehyde exposure and lavender oil have protective effects to the these damages.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: In our study, harmful effects of formaldehyde (FA) inhalation on the testes were investigated histologically.
📊
Key Findings & Data:Material-Methods: For this purpose, 21 albino-Wistar rats were used.
💡
Conclusion & Impact:Conclusion: It was shown that harmful effects of FA on testes occured via formaldehyde exposure and lavender oil have protective effects to the these damages.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: In our study, harmful effects of formaldehyde (FA) inhalation on the testes were investigated histologically. In addition, the possible protective effects of lavender oil which often used in aromatherapy, were examined against these damages. Material-Methods: For this purpose, 21 albino-Wistar rats were used. The rats of group I was used as control group. The rats of group II were exposed FA (10 ppm/ 1hour) for 35 days. The rats of group III inhaleted lavender oil (1ml/1 hour) with FA. At the end of the experiment, all rats were sacrified and testes were removed for histological examination. In this examination, the diameters of seminiferous tubul, Leydig cells and Leydig cells with damaged nucleus were determined. Results: When the rats were exposed to formaldehyde compared with the control group, it was determined that the diameter of tubuls and number of Leydig cells were decreased and Leydig cells with damaged nucleus were increased. And also it was seen that there were atrophic changes in the tubuls. In the group in which the lavender oil was applied with formaldehyde exposure it was determined that the histological changes of testes occured via FA exposure were improved. Conclusion: It was shown that harmful effects of FA on testes occured via formaldehyde exposure and lavender oil have protective effects to the these damages.

Özet

Amaç: Çalışmamızda, solunum yolu ile uygulanan formaldehit’in sıçan testislerinde yaptığı hasar histolojik olarak araştırıldı. Aynı zamanda, bu hasarlara karşı aromaterapi uygulamalarında sık kullanılan lavanta yağının muhtemel koruyucu etkileri de incelendi. Gereç-Yöntem: Bu amaçla, 21 adet Wistar-Albino cinsi erkek sıçan kullanıldı. Grup I’deki sıçanlar kontrol olarak kullanılırken, Grup II’deki sıçanlar, 35 gün boyunca solunum yoluyla formaldehit’e (10 ppm/1saat) maruz bırakıldı. Grup III’deki sıçanlara ise formaldehit uygulamasının yanı sıra saf lavanta yağı (1ml/1saat) yine solunum yoluyla verildi. Uygulama sonunda hayvanlar sakrifiye edildi ve histolojik incelemeler için testisler çıkarıldı. Çalışmada, tubuli seminiferi contorti çapları, Leydig hücre sayıları ve çekirdek hasarlı Leydig hücreleri incelendi. Bulgular: Formaldehit’e maruz kalan sıçanlar kontrol grubu ile karşılaştırıldığında, tubül çapları ve Leydig hücre sayılarında azalma, çekirdek hasarlı Leydig hücrelerinde ise artma tespit edildi. Ayrıca tubül duvarlarında atrofik değişiklikler gözlendi. Formaldehit maruziyetiyle birlikte lavanta yağı uygulanan grupta ise, formaldehit maruziyetine bağlı olarak testiste gelişen histolojik değişikliklerin düzelmiş olduğu belirlendi. Sonuç: Solunum yolu ile formaldehit maruziyetine bağlı olarak testiste önemli histolojik hasarların meydana geldiği ve bu hasarlara karşı lavanta yağının koruyucu özellik gösterdiği tespit edildi.

🔖

Cite This Article

ÖGETÜRK, M., KÖSE, E., MEYDAN, S., DABAK, D. Ö., PEKMEZ, H., SARSILMAZ, M., KAVAKLI, A. (2022). The histological evaluation of the testes of rats which applied formaldehyde and lavender oil. İnönü Üniversitesi Tıp Fakültesi Dergisi, Cilt: 17 - Sayı: 3, 169–173. https://ajindex.com/articles/548508

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Smith AE. Formaldehyde. Occup Med 1992; 42:83-8
  2. Zararsiz I, Kus I, Akpolat N, et al. Melatonin prevents formaldehyde-induced neurotoxicity in prefrontal cortex of rats: an immunohistochemical and biochemical study. Cell Biochem Funct 2007;25:413–8
  3. Blair A, Stewart PA, Hoover RN. Mortality from lung cancer among workers employed in formaldehyde industries. Am J Ind Med 1990;17:683-99
  4. Kim H, Kim YD, Cho SH. Formaldehyde exposure levels and serum antibodies to formaldehyde human serum albumin of Korean medical students. Arch Environ Health 1998;54:115-8
  5. Zararsiz I, Kus I, Akpolat N, et al. Protective effects of w-3 essential fatty acids against formaldehyde-induced neuronal damage in prefrontal cortex of rats. Cell Biochem Funct 2006;24:237-44
  6. Zararsiz I, Sönmez MF, Yilmaz HR et al. Effects of omega- 3 essential fatty acids against formaldehyde-induced nephropathy in rats. Toxicol Ind Health 2006; 22:223-29
  7. Sarsilmaz M, Kaplan S, Songur A, et al. Effects of postnatal formaldehyde exposure on pyramidal cell number, volume of cell layer in hippocampus and hemisphere in the rat: a stereological study. Brain Res 2007; 1145: 157-67
  8. Kriebel D, Myers D, Cheng M, et al. Short term effect of formaldehyde on peak expiratory flow and irritant symptoms. Arch Environ Health 2001;56:11-8
  9. Canbilen A, Sezen Ş, Avunduk MC, et al. Formaldehit ve toksik etkileri. Genel Tıp Dergisi 1999; 9:33-9
  10. Collins JJ, Ness R, Tyl RW, et al. A review of adverse pregnancy outcomes and formaldehyde exposure in human and animal studies. Regul Toxicol Pharmacol 2001; 34:17-34
  11. Taskinen H, Kyyronen P, Hemminki K, et al. Laboratory work and pregnancy outcome. J Occup Med 1994;36:311-9
  12. Martin WJ. A teratology study of inhaled formaldehyde in the rat. Reprod Toxicol 1990; 4:237-9
  13. Majumder PK, Kumar VL. Inhibitory effects of formaldehyde on the reproductive system of male rats. Indian J Physiol Pharmacol 1995;39:80-2
  14. Odeigah PG. Sperm head abnormalities and dominant lethal effects of formaldehyde in albino rats. Mutat Res 1997; 389:141- 8
  15. Sarsılmaz M, Özen O.A, Akpolat N, et al. Subakut dönemde solunan formaldehitin sıçanların Leydig hücreleri üzerindeki histopatolojik etkileri. Fırat Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 1999;13:37-40
  16. Sarsılmaz M, Özen O.A. Subkronik dönem boyunca formaldehit soluyan sıçanların leydig hücrelerindeki histopatolojik değişiklikler. Fırat Tıp Dergisi 2000;2:1-5
  17. Ergen A, Özkan G, Biçer A.