Journals / IUFS Journal of Biology / 2015 / Cilt: 74 - Sayı: 1

Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria

Calendula officinalis çiçek özütlerinin fenolik bileşenlerinin ve bitki kaynaklı patojen bakterilere karşı antibakteriyel etkilerinin incelenmesi

Pages
25–33
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Considering eco-safety and effectiveness, antibacterial activities of plant extracts would be important in phytopathogen control.

📌 3 Key Takeaways

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Objective & Hypothesis:Plant-derived compounds comprise diverse biological activities with different mechanisms of actions.
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Key Findings & Data:Flower methanol and aqueous extracts had activity against 4 and 5 strains, respectively out of 11 phytopathogens tested.
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Conclusion & Impact:Considering eco-safety and effectiveness, antibacterial activities of plant extracts would be important in phytopathogen control.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Plant-derived compounds comprise diverse biological activities with different mechanisms of actions. The aim of this study was to comparatively evaluate antibacterial activities of methanol and aqueous flower and leaf extracts of Calendula officinalis (pot marigold) on plant-borne pathogens, with total phenolic contents and analysis of phenolics. Flower methanol and aqueous extracts had activity against 4 and 5 strains, respectively out of 11 phytopathogens tested. The highest activity was obtained with methanol extract of the flower against E. amylovara and C. michiganensis in all strains tested with 256 and 512 ?g/mL minimum inhibitory concentrations, respectively. Total phenolic content of flower extracts were higher than leaf extracts, and the methanol extract of the flower had the highest total phenols among four extracts obtained. Two flower extracts with antibacterial activity were tested for phenolic content. Chlorogenic acid, caffeic acid, rutin, and salicylic acid were common in both methanol and aqueous flower extracts. Gallic acid was only present in methanol extract, whereas vanillic acid was present in the aqueous extract. The Higher antibacterial activity of the methanol extract may be correlated to the considerably higher relative rutin amount. Considering eco-safety and effectiveness, antibacterial activities of plant extracts would be important in phytopathogen control.

Özet

Bitki türevi bileşikler, çeşitli etki mekanizmaları ile biyolojik aktivite gösterebilirler. Bu çalışmanın amacı, Calendula officinalis (aynısafa) türüne ait bireylerin çiçek ve yapraklarından metanol ve su ile elde edilen özütlerin fenolik bileşen analizleri ile bitki kaynaklı patojenler üzerine antibakteriyel etkilerinin karşılaştırmalı olarak değerlendirilmesidir. Çiçeklerden metanol elde edilen özüt 11 test fitopatojenden 4'üne, su ile elde edilen özüt ise bu fitopatojenlerin 5'ine karşı antibakteriyel etki gösterdi. Çiçek metanol özütü 256 ve 512 ?g/mL minimum inhibisyon konsantrasyonları ile tüm test suşlarından E. amylovara ve C. michiganensis suşları üzerinde en yüksek etkiye sahipti. Çiçek özütlerinin toplam fenolik madde içeriği yaprak özütlerinden daha fazla idi. Çiçeklerden metanol ile elde edilen özüt, tüm özütler arasında en yüksek toplam fenolik madde içeriğine sahipti. Antibakteriyel etki gösteren her iki çiçek özütü de fenolik içerik açısından test edildi ve iki özütte de ortak klorojenik asit, kafeik asit, rutin ve salisilik asit tespit edildi. Test edilen fenolik bileşiklerden gallik asit sadece metanol özütünde bulunurken, vanilik asit ise sadece su özütünde belirlendi. Metanol ile elde edilen çiçek özütündeki rutin miktarının su ile elde edilen özüte göre çok daha fazla olduğu ve bu özütün yüksek antibakteriyel aktivitesinin rutin içeriği ile ilişkilendirilebilir. Ekolojik açıdan güvenli bitki özütlerinin antibakteriyel etkinliğinin saptanması fitopatojen kontrolü açısından önemlidir.

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Cite This Article

ŞAHİN, F. İ., LARÇİN, Ö., KÖRPE, D. A., İŞERİ, Ö. D. (2022). Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria. IUFS Journal of Biology, Cilt: 74 - Sayı: 1, 25–33. https://ajindex.com/articles/543994

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