This study concluded that the methanol extract obtained from the leaves of C. limon possess an antioxidant potential.
📌 3 Key Takeaways
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Objective & Hypothesis:This study was aimed to identify the phytochemistry and antioxidant activities of the methanol extract obtained from the leaves of Citrus limon (L.) Osbeck(CLM).
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Key Findings & Data:HPLC analyses revealed the presence of various phenolics (gallic acid, catechin, vanillic acid, coumaric, resveratrol) and flavonoids (rutin and apigenin).Qualitative analyses showed phenolics (302.91 μg EAG/mg), tannins (36.86±0.71μg ECT/mg) and flavonoids (19.77 ±0.06 μg EQ/mg) and coumarins.
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Conclusion & Impact:This study concluded that the methanol extract obtained from the leaves of C. limon possess an antioxidant potential.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
This study was aimed to identify the phytochemistry and antioxidant activities of the methanol extract obtained from the leaves of Citrus limon (L.) Osbeck(CLM). HPLC analyses revealed the presence of various phenolics (gallic acid, catechin, vanillic acid, coumaric, resveratrol) and flavonoids (rutin and apigenin).Qualitative analyses showed phenolics (302.91 μg EAG/mg), tannins (36.86±0.71μg ECT/mg) and flavonoids (19.77 ±0.06 μg EQ/mg) and coumarins. CLM exhibited a significant concentration-dependentin vitro antioxidant activity against DPPH radical and reducing power (FRAP test). This study concluded that the methanol extract obtained from the leaves of C. limon possess an antioxidant potential.
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Cite This Article
HAFSİA, B., SABAH, D., NOURA, S., NAJLA, H., ABDELFATTAH, E. (2022). Phytochemistryand AntioxidantActivities ofthe MethanolicExtract Obtained from the Leaves of Citrus limon(L.) Osbeck. International Journal of Secondary Metabolite, Cilt: 7 - Sayı: 1, 47–53. https://ajindex.com/articles/541645
.Sofowora, A., Ogunbodede, E., Onayade, A. (2013).The Role and Place of Medicinal Plants in the Strategies for Disease Prevention.Afr J Tradit Complement AlternMed., 10 (5), 210-229. [2].Solecki, R., Shanidar, I.V. (1975). Aneanderthal Flower Burial in Northern Iraq.Science,190(4217), 880-881. [3].Shoemaker, M., Hamilton, B., Dairkee, S.H., Cohen, I., Campbell,M.J. (2005).In vitroAnticancer Activity of Twelve Chinese Medicinal Herbs.Phytother.Res.,19, 649-651. [4].Farnsworth, N.R., Akerele, O., Bingel, A.S., Soejarto, D.D., Guo, Z. (1986). Places des plantes médicinales dans la thérapeutique.Bulletin de l'organisation mondiale de la santé.,64(2), 159-164. [5].Diab, K. (2016). In Vitro studies on Phytochemical Content, Antioxidant, Anticancer, Immunomodulatory, and Antigenotoxic Activities.Asian Pac J Cancer Prev.,17(7), 3559-3567. [6].Wu, T., Luo, J., Xu B. (2015). In vitro Antidiabetic Effects of Selected Fruits and Vegetables Against Glycosidase and Aldose Reductase.Food Science and Nutrition.,3(6), 459-505. [7].Belletti, N., Ndagijimana, M., Sisto, C., Guerzoni, M. E., Lanciotti, R., Gardini, F. (2004). Evaluation of theAntimicrobial Activity of Citrus Essences on Saccharomy Cescerevisiae. J. Agric. Food Chem.,52, 6932-6938. [8].Jing, L., Lei, Z., Li, L., Xie, R., Xi, W., Guan, Y., Zhou, Z. (2014). Antifungal Activityof Citrus Essential Oils.J Agric Food Chem.,62(14), 3011-3033. [9].Bouzenna,H., Dhibi, S., Samout, N.,Rjeibi,I.,Talarmin, H.,Elfeki, A.,Hfaiedh, N.(2016). The Protective Effect of Citrus LimonEssential Oil on Hepatotoxicity and Nephrotoxicity Induced by Aspirin in Rats. Biomed. Pharmacother.,83, 1327-1334. [10].Bouzenna, H., Hfaiedh, N., Giroux-Metges, M-A., Elfeki, A., Talarmin, H. (2017). Protective Effects of Essential Oil of Citrus LimonagainstAspirin-Induced Toxicity in IEC-6 Cells.Appl Physiol Nutr Metab.,15, 479-486. [11].Bouzenna, H., Samout,N., Dhibi,S., Mbarki,S., Akermi, S., Khdhiri, A., Elfeki, A., Hfaiedh, N. (2018). Protective Effect of Essential Oil from Citrus LimonagainstAspirin-Induced Toxicity in Rats.Hum Exp Toxicol.,1-11. [12].Ravishankara, M.N., Shrivastava, N., Padh, H., Rajani, M. (2002). Evaluation of Antioxidant Properties of Root Bark of Hemidesmesindicus.Phytomedicine. 9, 153-60. [13].Singleton,V.L.,Rossi,J.A.(1965).ColorimetryofTotalPhenolicswithPhosphomolybdic PhosphotungsticAcid Reagents. Am j Enol Viticult.,16, 144-158. [14].Dewanto, V., Wu, X., Adom, K.K., Liu, R.H. (2002). Thermal Processing Enhances the