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Journals / International Journal of Secondary Metabolite / 2020 / Cilt: 7 - Sayı: 1

Cytotoxic and Apoptotic Activities of Rhizopogon roseolus (Corda) Th.Fr. Extracts

Ali ÖZMEN
Erdi Can AYTAR
Pages
54–62
Publication Date
2022-10-19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Both of the extracts were shown higher apoptotic effect than necrotic effect on the HL-60 cell line.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Many species of mushrooms have been used since ancient times, especially in Asian countries, as a food supplement and in the medical field due to their different biological activities.
📊
Key Findings & Data:The methanol and water extracts of mushroom were tested against HL- 60 human cancer cell line.
💡
Conclusion & Impact:Both of the extracts were shown higher apoptotic effect than necrotic effect on the HL-60 cell line.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Rhizopogon roseolus · Mushroom · Apoptosis

Abstract

Many species of mushrooms have been used since ancient times, especially in Asian countries, as a food supplement and in the medical field due to their different biological activities. Nowadays, especially in Japan, Korea and China, various mushroom extracts have been used as potential additives in chemotherapy and radiation treatments. In this study, anticancer activity and apoptotic effect of Rhizopogon roseolus were investigated. The methanol and water extracts of mushroom were tested against HL- 60 human cancer cell line. Antiproliferative effects of the extracts were evaluated by using MTT method and apoptosis and necrosis ratios of the cells treated with extracts were determined by using Hoechst/Propidium iodide (HO/PI) staining method. According to obtained data, antiprolifarative effect of the methanol extract was higher than water extract and this effect was a concentration depending manner. Both of the extracts were shown higher apoptotic effect than necrotic effect on the HL-60 cell line.

Özet

Many species of mushrooms have been used since ancient times, especially in Asian countries, as a food supplement and in the medical field due to their different biological activities. Nowadays, especially in Japan, Korea and China, various mushroom extracts have been used as potential additives in chemotherapy and radiation treatments. In this study, anticancer activity and apoptotic effect of Rhizopogon roseolus were investigated. The methanol and water extracts of mushroom were tested against HL- 60 human cancer cell line. Antiproliferative effects of the extracts were evaluated by using MTT method and apoptosis and necrosis ratios of the cells treated with extracts were determined by using Hoechst/Propidium iodide (HO/PI) staining method. According to obtained data, antiprolifarative effect of the methanol extract was higher than water extract and this effect was a concentration depending manner. Both of the extracts were shown higher apoptotic effect than necrotic effect on the HL-60 cell line.

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Cite This Article

ÖZMEN, A., AYTAR, E. C. (2022). Cytotoxic and Apoptotic Activities of Rhizopogon roseolus (Corda) Th.Fr. Extracts. International Journal of Secondary Metabolite, Cilt: 7 - Sayı: 1, 54–62. https://ajindex.com/articles/541633

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