SavedAdd your journal

Journals / International Journal of Environment and Geoinformatics / 2021 / Cilt: 8 - Sayı: 1

Identification of Sea cucumber Holothuria (Lessonothuria) pardalis (Selenka, 1867) andHolothuria (Semperothuria) cinerascens (Brandt, 1835) (Family-Holothuriidae) basedon morphological and mitochondrial DNA evidence and phylogenetic analysis fromKarachi Coast, Pakistan

Quratulan AHMED
Qadeer Mohammad ALI
Angus HH MACDONALD
Pages
57–64
Publication Date
2022-10-19
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study is the first to report on molecular identification of H.pardalis and H. cinerascens based on the 16SrRNAand COI gene from the Karachi Coast, Pakistan.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The conventional taxonomy on sea cucumbers is challenging due to their morphological complexity.
📊
Key Findings & Data:This is the first research describing the molecular identification of H. pardalis and H. cinerascens based on 16S rRNAandCOIgenes from the Karachi Coast of Pakistan.
💡
Conclusion & Impact:This study is the first to report on molecular identification of H.pardalis and H. cinerascens based on the 16SrRNAand COI gene from the Karachi Coast, Pakistan.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The conventional taxonomy on sea cucumbers is challenging due to their morphological complexity. Molecular investigation of the class Holothuroidea was initiated for further clarity regarding the systematics and taxonomy of this class. A molecular phylogeny ofthe Holothuroidea was constructed by using maximum likelihood methods, and this unveiled variation in the existing taxonomic classification, which is largely based on the morphology of calcareous parts. H. pardalis and H. cinerascens from Pakistan was identified morphologically but no information was found with regard to molecular identification. This is the first research describing the molecular identification of H. pardalis and H. cinerascens based on 16S rRNAandCOIgenes from the Karachi Coast of Pakistan. Comparisons of mitochondrial 16S rRNAand COI to accessioned sea cucumber sequences on GenBank confirmed H.pardalis’and H.cinerascens identity. This study is the first to report on molecular identification of H.pardalis and H. cinerascens based on the 16SrRNAand COI gene from the Karachi Coast, Pakistan.

🔖

Cite This Article

AHMED, Q., ALI, Q. M., MACDONALD, A. H. (2022). Identification of Sea cucumber Holothuria (Lessonothuria) pardalis (Selenka, 1867) andHolothuria (Semperothuria) cinerascens (Brandt, 1835) (Family-Holothuriidae) basedon morphological and mitochondrial DNA evidence and phylogenetic analysis fromKarachi Coast, Pakistan. International Journal of Environment and Geoinformatics, Cilt: 8 - Sayı: 1, 57–64. https://ajindex.com/articles/540924

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (6)

  1. Ahmed, Q., Ali, Q.M.,(2014). Abundance and Distribution of Holothuroidea (Echinodermata) with Emphasis on Heavy Metals Accumulation in Organism and Its Habitat, Project: Higher Education Commission of Pakistan, (Grant No. IPFP/HRD/HEC/1688). Ahmed,Q., Ahmed S. T and Ali, QM. (2020). Holothuria (Lessonothuria) insignis Ludwig, 1875 (formally resurrected from synonymy of H. pardalisSelenka, 1867) and Holothuria (Lessonothuria) lineate Ludwig, 1875—new additions to the sea cucumber fauna of Pakistan, with a key to the subgenus LessonothuriaDeichmann (Echinodermata: Holothuroidea) Zootaxa4767 (2): 307–318. Ahmed,Q., Ali,QM., and Conand, C. (2016). New additions to the holothurian fauna of Pakistan: Holothuriaverrucosa, Holothuriacinerascensand Ohshimellaehrenbergii, SPC Beche-de-mer Information Bulletin #36; 20-23.
  2. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J. (1990). Basic local alignment search tool. J Mol Biol. 215(3):403-10.
  3. Atif, M., El-Naggar, Neveen, A., Ashaat, Hussein, I., ElBelbasi, Mohamed, S.S.,(2008).Molecular Phylogeny of Egyptian Sea Cucumbers As Predicted From 16s Mitochondrial rRNA Gene Sequences World Alied Sciences Journal, 5(5): 531-542.
  4. Clark, A.M., Rowe, F.W.E.,(1971).Monograph of shallow-water Indo-West Pacific echinoderms. London: Trustees of the British Museum (Natural History). 238 p. Grube A.E. 1840.Actinien, Echinodermen und Würmer des Adriatischen- und Mittelmeers, nacheigenenSammlungenbeschrieben. p. 1–92. J.H. Bon. Königsberg (available online at http://www.biodiversitylibrary.org/bibliography/1013 3). Clouse, R., Janies, D., & Kerr, A. M. (2005). Resurrection of BohadschiabivittatafromB. marmorata(Holothuroidea: Holothuriidae) based on behavioral, morphological, and mitochondrial DNA evidence. Zoological, 108(1), 27-39.
  5. Ehsanpour, Z., ArchangiB., Mona, S., Ali, S.M., ZolgharneinH.,(2012). Morphological and Molecular Identification of Holothuria (Selenkothuria) parva from Bostaneh Port, Persian Gulf.Indian journal of Geo-Marine Sciences.45:405-409.
  6. Freeland, J.R.,(2005).Molecular Ecology.John Wiley & Sons, Ltd. 2005. ISBN 978-0-470-09062-6 400. Giomar Helena Borrero-Pérez A, Jesús Gómez-Zurita B, Mercedes González-Wangüemert A,Concepción Marcos A, Angel Pérez-Ruzafa (2010). Molecular systematics of the genus Holothuria in the Mediterranean and Northeastern Atlantic and a molecular clock for the diversification of the Holothuriidae (Echinodermata: Holothuroidea), Molecular Phylogenetics and Evolution 57, 899–90