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Journals / International Journal of Environment and Geoinformatics / 2021 / Cilt: 8 Sayı: 1

Identification of Sea Cucumber Holothuria (Lessonothuria) Pardalis (Selenka, 1867) and Holothuria (Semperothuria) Cinerascens (Brandt, 1835) (Family-Holothuriidae) based on Morphological and Mitochondrial DNA Evidence and Phylogenetic Analysis from Karachi Coast, Pakistan

Quratulan AHMED
Qadeer Mohammad ALİ
Angus Hh MACDONALD
Pages
57–64
Publication Date
2021-03-07
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study is the first to report on molecular identification of H.pardalis and H. cinerascens based on the 16S rRNAand COI gene from the Karachi Coast, Pakistan.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The conventional taxonomy on sea cucumbers is challenging due to their morphological complexity.
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Key Findings & Data:This is the first research describing the molecular identification of H. pardalis and H. cinerascens based on 16S rRNA and COI genes from the Karachi Coast of Pakistan.
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Conclusion & Impact:This study is the first to report on molecular identification of H.pardalis and H. cinerascens based on the 16S rRNAand COI gene from the Karachi Coast, Pakistan.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sea cucumber · DNA barcoding · Holothuroidea

Abstract

The conventional taxonomy on sea cucumbers is challenging due to their morphological complexity. Molecular investigation of the class Holothuroidea was initiated for further clarity regarding the systematics and taxonomy of this class. A molecular phylogeny of the Holothuroidea was constructed by using maximum likelihood methods, and this unveiled variation in the existing taxonomic classification, which is largely based on the morphology of calcareous parts. H. pardalis and H. cinerascens from Pakistan was identified morphologically but no information was found with regard to molecular identification. This is the first research describing the molecular identification of H. pardalis and H. cinerascens based on 16S rRNA and COI genes from the Karachi Coast of Pakistan. Comparisons of mitochondrial 16S rRNA and COI to accessioned sea cucumber sequences on GenBank confirmed H.pardalis’ and H. cinerascens identity. This study is the first to report on molecular identification of H.pardalis and H. cinerascens based on the 16S rRNAand COI gene from the Karachi Coast, Pakistan.

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Cite This Article

AHMED, Q., ALİ, Q. M., MACDONALD, A. H. (2021). Identification of Sea Cucumber Holothuria (Lessonothuria) Pardalis (Selenka, 1867) and Holothuria (Semperothuria) Cinerascens (Brandt, 1835) (Family-Holothuriidae) based on Morphological and Mitochondrial DNA Evidence and Phylogenetic Analysis from Karachi Coast, Pakistan. International Journal of Environment and Geoinformatics, Cilt: 8 Sayı: 1, 57–64. https://ajindex.com/articles/929677

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References (7)

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